FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5640756
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG167770229.742502600715227No Hit
CCCCCACTGCTAAATTTGACTG3908236.928557094120008No Hit
CAACGGAATCCCAAAAGCAGCTG3894556.904305025780233No Hit
AACATTCAACGCTGTCGGTGAGT1467062.6008215919993702No Hit
TTCAAGTAATCCAGGATAGGCT1276092.262267681849738No Hit
TAGCAGCACGTAAATATTGGCG1089081.930734107272146No Hit
AAGCTGCCAGTTGAAGAACTGT975101.7286689940142774No Hit
AACATTCAACGCTGTCGGTGAG852331.5110208631608955No Hit
TATTGCACTTGTCCCGGCCTGTAA483330.8568532303116817No Hit
TAGCTTATCAGACTGATGTTGACT471910.8366077171216056No Hit
TAGCTTATCAGACTGATGTTGAC461350.8178868222628314No Hit
TATTGCACTTGTCCCGGCCTGTAT425990.7552001894781479No Hit
TGAGGTAGTAGGTTGTATAGTT319780.5669098255623892No Hit
ACCCCCCACTGCTAAATTTGACTG289590.5133886308856472No Hit
TAGCTTATCAGACTGATGTTGA265330.47038021144683445No Hit
TGTAAACATCCTTGACTGGAAGCT255770.4534321286012017No Hit
TATTGCACTTGTCCCGGCCTGT249150.441696113074205No Hit
CAACGGAATCCCAAAAGCAGCTGT230400.40845588782780184No Hit
TATTGCACTTGTCCCGGCCTGTA225540.39984002144393416No Hit
CCCCCCACTGCTAAATTTGACTGG210640.37342512244812576No Hit
GACCCGCGGGCGCTCTCCAGTCCT208310.3692944704575061No Hit
CCCATAAAGTAGAAAGCACTAC204260.36211458180428296No Hit
CCCCCCACTGCTAAATTTGACTGT199710.3540482871444891No Hit
TCGTACGACTCTTAGCGG179290.31784746583613965No Hit
AGCAGCATTGTACAGGGCTATGA178480.3164114881054951No Hit
CATAAAGTAGAAAGCACTACT168580.29886064917539423No Hit
ACTGGACTTGGAGTCAGAAGGC165710.2937726786976781No Hit
TTCAAGTAATTCAGGATAGGTT163820.29042206399284065No Hit
TGAGGTAGTAGATTGTATAGTT156710.2778173705794046No Hit
CGCCCCACTGCTAAATTTGACTG144080.2554267548534275No Hit
TTCTCACTACTGCACTTGACTAG142900.25333483667792045No Hit
CTTCTCACTACTGCACTTGACTAG138690.24587129810259475No Hit
TTAATGCTAATCGTGATAGGGGTT136230.24151018055026666No Hit
TGTAAACATCCCCGACTGGAAGCT136030.2411556181476384No Hit
CTCCCACTGCTTCACTTGACTAG133120.2359967351893966No Hit
CCCCCCACTGCTAAATTTGACTGTA129840.2301819117862925No Hit
GCCCCCACTGCTAAATTTGACTG120110.2129324508984257No Hit
CCCCCACTGCTAAATTTGACTGGCTT114140.2023487631799709No Hit
TGTAAACATCCTACACTCTCAGCT110830.19648075541647253No Hit
CACCCGGCTGTGTGCACATGTGC107480.19054183517244852No Hit
CCCGCCACTGCTAAATTTGACTG104400.18508157417197268No Hit
CAAAGAATTCTCCTTTTGGGCT102870.18236917179186618No Hit
CATTGCACTTGTCTCGGTCTGA98950.1754197487003515No Hit
TAGCAGCACATCATGGTTTACA95910.17003040018040136No Hit
CCCCCCACTGCTAAATTTGACTGGCT95540.169374459735539No Hit
GTCAGGATGGCCGAGCGGTCTAAGG93040.16494242970268524No Hit
CCCTCCACTGCTAAATTTGACTG90070.159677178023655No Hit
AACATTCAACGCTGTCGGTGAGTT89560.15877304389695282No Hit
CCCCCCACTGCTAAATTTGACTGA88470.15684067880262859No Hit
CCCATAAAGTAGAAAGCACT86490.1533305110166084No Hit
TAGCTTATCAGACTGATGTTGACA79480.14090309880448648No Hit
CTACGCCTGTCTGAGCGTCGCT76250.13517691600203943No Hit
CGCCTGTCTGAGCGTCGCT75600.13402458819349747No Hit
AACGGAATCCCAAAAGCAGCTG74170.13148946701470512No Hit
CTCCCACTGCTTCACTTGACTAGCCT73960.1311171764919454No Hit
TCGTTTCCCGGCCAATGCACCA72140.12789065862802787No Hit
ATCACATTGCCAGGGATTTCC68780.12193401026387243No Hit
CAACGGAATCCCAAAAGCAGCT67220.1191684235233717No Hit
TAGCAGCACGTAAATATTGGC66610.11808700819535539No Hit
TGAGGTAGTAGTTTGTACAGTT64250.11390317184434143No Hit
TATTGCACTTGTCCCGGCCTGTAG63450.11248492223382824No Hit
TTCAAGTAATCCAGGATAGGC63280.11218354419159418No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC63080.11182898178896587No Hit
AGCTCGGTCTGAGGCCCCTCAGT62710.11117304134410351No Hit
TCGTTTCCCGGGCGGCGCACCA60980.10810607656136872No Hit
CACTAGATTGTGAGCTCCTGGAA59440.1053759460611308No Hit
CACTAGATTGTGAGCTCCTGGA58070.10294719360312696No Hit
CCCCCCACTGCTAAATTTGAC57900.1026458155608929No Hit
ACCGGGTGCTGTAGGCTT56940.10094391602827706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)