FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9489649
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG269857228.437005415058024No Hit
CCCCCACTGCTAAATTTGACTG9085539.574147579114886No Hit
CAACGGAATCCCAAAAGCAGCTG4835505.095552006191166No Hit
TAGCAGCACGTAAATATTGGCG2487932.621730266314381No Hit
TTCAAGTAATCCAGGATAGGCT2440802.5720656264525696No Hit
AACATTCAACGCTGTCGGTGAGT2270352.392448867181494No Hit
AAGCTGCCAGTTGAAGAACTGT2016162.1245885912113294No Hit
AACATTCAACGCTGTCGGTGAG1706931.798728277515849No Hit
TAGCTTATCAGACTGATGTTGAC1074561.1323495737302824No Hit
TAGCTTATCAGACTGATGTTGACT1062791.1199465860117692No Hit
TAGCTTATCAGACTGATGTTGA1054261.1109578447000517No Hit
TATTGCACTTGTCCCGGCCTGTAT707920.7459917642897014No Hit
TGAGGTAGTAGGTTGTATAGTT696640.7341051286512283No Hit
TATTGCACTTGTCCCGGCCTGTAA690610.7277508367274701No Hit
TGTAAACATCCTTGACTGGAAGCT610800.6436486744662527No Hit
CATAAAGTAGAAAGCACTACT587640.6192431353361962No Hit
TATTGCACTTGTCCCGGCCTGT570950.601655551222179No Hit
TGAGGTAGTAGATTGTATAGTT512380.5399356709610651No Hit
TATTGCACTTGTCCCGGCCTGTA467360.49249450638269127No Hit
TTCTCACTACTGCACTTGACTAG461500.4863193570173143No Hit
TTCAAGTAATTCAGGATAGGTT441540.4652859131038461No Hit
AGCAGCATTGTACAGGGCTATGA333120.3510351120468207No Hit
CGCCTGTCTGAGCGTCGCT320790.3380420076654047No Hit
CCCATAAAGTAGAAAGCACT301570.3177883607707725No Hit
GCCTGTCTGAGCGTCGCT295280.3111600861106665No Hit
CTTCTCACTACTGCACTTGACTAG291320.3069871182801387No Hit
GGAATACCGGGTGCTGTAGGCTTT284030.29930506386484895No Hit
TGTAAACATCCCCGACTGGAAGCT265470.279746911608638No Hit
CATTGCACTTGTCTCGGTCTGA264110.27831377114158806No Hit
ACTGGACTTGGAGTCAGAAGGC258470.27237045332235155No Hit
TGAGGTAGTAGTTTGTACAGTT237840.25063097697291015No Hit
GACCCGCGGGCGCTCTCCAGTCCT224920.23701614253593575No Hit
GGAATACCGGGTGCTGTAGGCTT224550.23662624402651772No Hit
CAACGGAATCCCAAAAGCAGCTGT212920.22437078547373038No Hit
CCCATAAAGTAGAAAGCACTAC198680.20936496175991337No Hit
CCCCCCACTGCTAAATTTGACTGG196580.20715202427402743No Hit
ACCCCCCACTGCTAAATTTGACTG185110.19506517048206948No Hit
TGTAAACATCCTACACTCTCAGCT183010.1928522329961835No Hit
CCCCCCACTGCTAAATTTGACTGT179480.1891323904603848No Hit
CAACGGAATCCCAAAAGCAGCT173530.18286240091704128No Hit
TTAATGCTAATCGTGATAGGGGTT172780.18207206610065346No Hit
CAACACCAGTCGATGGGCTGT171800.18103936194057335No Hit
ACCGGGTGCTGTAGGCTT171740.18097613515526234No Hit
TCGTACGACTCTTAGCGG171390.180607312240948No Hit
CAAAGAATTCTCCTTTTGGGCT146310.15417851598093882No Hit
ACCGGGTGCTGTAGGCTTA143400.151112016893354No Hit
TTCACAGTGGCTAAGTTCCG133450.1406269083292754No Hit
AACATTCAACGCTGTCGGTGAGTT131960.13905677649405157No Hit
ACCGGGTGCTGTAGGCTTT130810.13784492977559024No Hit
TGATGTAGTAGGTTGTATAGTT130650.13767632501476082No Hit
CACCCGGCTGTGTGCACATGTGC130300.13730750210044648No Hit
CTCCCACTGCTTCACTTGACTAG129400.13635910032078108No Hit
TTATAAAGCAATGAGACTGATT124930.1316487048151096No Hit
TAGCAGCACGTAAATATTGGC122250.12882457507121708No Hit
TTCACAGTGGCTAAGTTCTG121810.1283609119789362No Hit
TGAGGTAGTAGTTTGTGCTGTT118030.12437762450434153No Hit
CCCCCCACTGCTAAATTTGACTGA117170.12347137391488347No Hit
CGCCCCACTGCTAAATTTGACTG115150.12134273880941224No Hit
TTCAAGTAATCCAGGATAGGC114080.12021519447136558No Hit
AACGGAATCCCAAAAGCAGCTG113800.11992013613991413No Hit
TAGCTTATCAGACTGATGTTGACA113280.11937217066721856No Hit
TCCTGTCTGAGCGTCGCT113200.11928786828680386No Hit
TGTCAGTTTGTCAAATACCCCA111650.117654509666269No Hit
TGAATACCGGGTGCTGTAGGCTTT111620.11762289627361348No Hit
GCCCCCACTGCTAAATTTGACTG110950.11691686383764036No Hit
TTCAAGTAATCCAGGATAGGCTT109200.11507274926606875No Hit
TATTGCACTTGTCCCGGCCTGTT107510.11329186147980815No Hit
CTACGCCTGTCTGAGCGTCGCT106560.11229077071238357No Hit
TGTCAGTTTGTCAAATACCCCAA104920.11056257191388216No Hit
CACTAGATTGTGAGCTCCTGGA104760.11039396715305276No Hit
CCCCCCACTGCTAAATTTGACTGTA100320.10571518504003678No Hit
CCCGCCACTGCTAAATTTGACTG99760.10512506837713387No Hit
TGATGTAGTAGATTGTATAGTT96410.10159490619726821No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)