FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7578798
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG179733923.715356973493687No Hit
CCCCCACTGCTAAATTTGACTG5230536.901529767649171No Hit
CAACGGAATCCCAAAAGCAGCTG4687236.184661472703191No Hit
TAGCAGCACGTAAATATTGGCG2272002.9978368601458962No Hit
TTCAAGTAATCCAGGATAGGCT1934082.551961406017155No Hit
AACATTCAACGCTGTCGGTGAGT1746452.304389165669807No Hit
AAGCTGCCAGTTGAAGAACTGT1605972.11902995699318No Hit
AACATTCAACGCTGTCGGTGAG1274851.682126901917692No Hit
TAGCTTATCAGACTGATGTTGACT1007291.3290893885811446No Hit
TAGCTTATCAGACTGATGTTGAC971591.281984293551563No Hit
TATTGCACTTGTCCCGGCCTGTAT941681.2425189324217376No Hit
TATTGCACTTGTCCCGGCCTGTAA853631.126339559386594No Hit
TAGCTTATCAGACTGATGTTGA743930.9815936511304298No Hit
TGTAAACATCCTTGACTGGAAGCT632970.8351852101085159No Hit
TGAGGTAGTAGGTTGTATAGTT567180.7483772492682876No Hit
TATTGCACTTGTCCCGGCCTGT560640.7397479125317762No Hit
TATTGCACTTGTCCCGGCCTGTA509770.6726264507907455No Hit
TGAGGTAGTAGATTGTATAGTT480210.6336229043180726No Hit
CATAAAGTAGAAAGCACTACT471340.6219192014353727No Hit
TTCTCACTACTGCACTTGACTAG376890.49729521752657874No Hit
TTCAAGTAATTCAGGATAGGTT372120.49100134348481117No Hit
GGAATACCGGGTGCTGTAGGCTTT371200.4897874306717239No Hit
AGCAGCATTGTACAGGGCTATGA363270.47932403001109153No Hit
TGTAAACATCCCCGACTGGAAGCT298220.3934924773031291No Hit
GACCCGCGGGCGCTCTCCAGTCCT292210.3855624599045917No Hit
CCCATAAAGTAGAAAGCACTAC288070.38009985224569914No Hit
ACTGGACTTGGAGTCAGAAGGC283310.37381917290842165No Hit
CTTCTCACTACTGCACTTGACTAG267130.3524701410434742No Hit
GGAATACCGGGTGCTGTAGGCTT261930.3456088947086332No Hit
ACCCCCCACTGCTAAATTTGACTG249920.32976205461604857No Hit
CCCATAAAGTAGAAAGCACT239080.3154589949488032No Hit
CAACGGAATCCCAAAAGCAGCTGT234310.30916512090703563No Hit
CATTGCACTTGTCTCGGTCTGA228490.30148580289380983No Hit
CCCCCCACTGCTAAATTTGACTGA201980.26650664129061097No Hit
TGTAAACATCCTACACTCTCAGCT201570.26596565840651776No Hit
TCGTACGACTCTTAGCGG193310.2550668324977127No Hit
CTCCCACTGCTTCACTTGACTAG186640.24626596460283015No Hit
TGAGGTAGTAGTTTGTACAGTT181830.23991931174310227No Hit
CCCCCCACTGCTAAATTTGACTGG181650.23968180706228087No Hit
CAAAGAATTCTCCTTTTGGGCT173040.228321166496323No Hit
CCCCCCACTGCTAAATTTGACTGT156680.20673462995055417No Hit
TGTCAGTTTGTCAAATACCCCAA156410.20637837292932204No Hit
ACCGGGTGCTGTAGGCTTA144710.19094056867592987No Hit
AGCTCGGTCTGAGGCCCCTCAGT144550.19072945340408862No Hit
ACCGGGTGCTGTAGGCTT137310.18117648735327158No Hit
TAGCTTATCAGACTGATGTTGACA134310.17721807600624795No Hit
CACTAGATTGTGAGCTCCTGGAA133930.17671667723562495No Hit
CACTAGATTGTGAGCTCCTGGA130910.17273187647962115No Hit
TGTCAGTTTGTCAAATACCCCA130700.1724547876853295No Hit
TATTGCACTTGTCCCGGCCTGTT126960.16751996820604007No Hit
TATTGCACTTGTCCCGGCCTGTAG126300.16664911770969487No Hit
AACATTCAACGCTGTCGGTGAGTT125380.16543520489660762No Hit
CCCCCACTGCTAAATTTGACTGGCTT122180.1612128994597824No Hit
CACCCGGCTGTGTGCACATGTGC121610.1604608013038479No Hit
AACGGAATCCCAAAAGCAGCTG119730.15798019685971312No Hit
TAGCAGCACGTAAATATTGGC116770.1540745643306498No Hit
ACCGGGTGCTGTAGGCTTT115020.15176549104488601No Hit
CCCCCCACTGCTAAATTTGACTGTA109920.14503619175494584No Hit
CGCCTGTCTGAGCGTCGCT108470.1431229596038844No Hit
TGAGGTAGTAGTTTGTGCTGTT107680.1420805779491682No Hit
TTCACAGTGGCTAAGTTCCG105530.13924371648380127No Hit
CTTCTCACTACTGCACTTGACTA105170.1387687071221584No Hit
CCCCCCACTGCTAAATTTGACTGGCT104230.13752840490009102No Hit
CAACACCAGTCGATGGGCTGT101650.1341241711416507No Hit
TTCAAGTAATCCAGGATAGGC98980.13060118504279966No Hit
CCCCCCACTGCTAAATTTGAC97650.12884628934561917No Hit
TCAGTGCACTACAGAACTTTGT97120.128146970007645No Hit
TTCAAGTAATCCAGGATAGGCTT96610.127474040078651No Hit
TGAATACCGGGTGCTGTAGGCTTT95110.12549483440513917No Hit
CCCCCACAACCGCGCTTGACTAG90300.11914818154541129No Hit
CTCCCACTGCTTCACTTGACTA90250.1190822080229609No Hit
CAAAGAATTCTCCTTTTGGGCTT90180.11898984509153034No Hit
TGTAAACATCCCCGACTGGAAGC90040.11880511922866925No Hit
TATTGCACTTGTCCCGGCCTGTTT87250.11512379667593726No Hit
CCCATAAAGTAGAAAGCACTA83500.11017578249215773No Hit
TTCACAGTGGCTAAGTTCTG80320.10597986646431268No Hit
CAACGGAATCCCAAAAGCAGCT76770.10129574637033471No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)