FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8216222
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG113481713.81190771135444No Hit
CAACGGAATCCCAAAAGCAGCTG6084917.405970773428469No Hit
CCCCCACTGCTAAATTTGACTG3361814.091673764413863No Hit
TTCAAGTAATCCAGGATAGGCT2519413.0663850124789716No Hit
TAGCAGCACGTAAATATTGGCG2501373.044428448987868No Hit
AAGCTGCCAGTTGAAGAACTGT2048012.492641995311227No Hit
AACATTCAACGCTGTCGGTGAGT2023792.4631637265886925No Hit
TAGCTTATCAGACTGATGTTGACT2007012.4427407146496285No Hit
TAGCTTATCAGACTGATGTTGAC1965832.3926203551948815No Hit
AACATTCAACGCTGTCGGTGAG1416591.7241379310344827No Hit
TAGCTTATCAGACTGATGTTGA1314411.5997742027905284No Hit
TATTGCACTTGTCCCGGCCTGTAT1290861.5711113940202686No Hit
TATTGCACTTGTCCCGGCCTGTAA1260341.5339653675375373No Hit
TGAGGTAGTAGGTTGTATAGTT943391.1482041259352533No Hit
TGTAAACATCCTTGACTGGAAGCT878951.0697739179880972No Hit
TGAGGTAGTAGATTGTATAGTT690630.8405688161785307No Hit
TTCAAGTAATTCAGGATAGGTT604500.7357396136569825No Hit
TATTGCACTTGTCCCGGCCTGT586270.7135518003286669No Hit
AGCAGCATTGTACAGGGCTATGA509880.6205771947252642No Hit
TGTAAACATCCCCGACTGGAAGCT509050.6195669980679686No Hit
TATTGCACTTGTCCCGGCCTGTA496400.6041706273272557No Hit
CCCATAAAGTAGAAAGCACTAC449490.5470762596239488No Hit
GGAATACCGGGTGCTGTAGGCTTT397430.4837138042277826No Hit
CATAAAGTAGAAAGCACTACT370910.45143619537057295No Hit
ACTGGACTTGGAGTCAGAAGGC366970.446640804009434No Hit
CAACGGAATCCCAAAAGCAGCTGT343850.418501350133918No Hit
CTCCCACTGCTTCACTTGACTAG322840.3929299865558647No Hit
TGTAAACATCCTACACTCTCAGCT306650.37322506621656526No Hit
TAGCTTATCAGACTGATGTTGACA282160.34341817930430796No Hit
CCCCCCACTGCTAAATTTGACTGGCT279600.34030239202397405No Hit
TCGTACGACTCTTAGCGG277000.33713792056738484No Hit
CCCCCACTGCTAAATTTGACTGGCTT265490.32312904884994587No Hit
TGAGGTAGTAGTTTGTACAGTT260920.31756688171278724No Hit
GGAATACCGGGTGCTGTAGGCTT242020.2945636084321967No Hit
CAAAGAATTCTCCTTTTGGGCT241520.2939550562290065No Hit
CCCATAAAGTAGAAAGCACT237400.288940586074719No Hit
CATTGCACTTGTCTCGGTCTGA235940.2871636136414035No Hit
ACCCCCCACTGCTAAATTTGACTG227710.27714684437689246No Hit
CACCCGGCTGTGTGCACATGTGC226080.2751629641944923No Hit
CACTAGATTGTGAGCTCCTGGAA225710.27471263556413156No Hit
CTTCTCACTACTGCACTTGACTAG212370.25847646278301634No Hit
CACTAGATTGTGAGCTCCTGGA211410.2573080425528911No Hit
TTCTCACTACTGCACTTGACTAG210460.2561517933668297No Hit
CTCCCACTGCTTCACTTGACTAGCCT201420.24514916953315039No Hit
CCCCCCACTGCTAAATTTGACTGG200420.24393206512676996No Hit
CCCCCCACTGCTAAATTTGACTGT184250.22425148687559804No Hit
TATTGCACTTGTCCCGGCCTGTAG181450.2208435945377328No Hit
CAACACCAGTCGATGGGCTGT159620.19427420534644757No Hit
TTCAAGTAATCCAGGATAGGCTT154440.18796960452139683No Hit
TGAGGTAGTAGTTTGTGCTGTT154160.1876288152876103No Hit
AGCTCGGTCTGAGGCCCCTCAGT151340.18419658086161741No Hit
CAAAGAATTCTCCTTTTGGGCTT149830.18235875320798295No Hit
AACGGAATCCCAAAAGCAGCTG140560.17107619536083615No Hit
AACATTCAACGCTGTCGGTGAGTT140530.17103968222864474No Hit
TGTCAGTTTGTCAAATACCCCAA137590.1674613952738862No Hit
TAGCAGCACGTAAATATTGGC135860.16535580465084804No Hit
TGTCAGTTTGTCAAATACCCCA132030.1606942947744109No Hit
TATTGCACTTGTCCCGGCCTGTT129870.15806534925662913No Hit
TTCAAGTAATCCAGGATAGGC129650.15779758628722546No Hit
TCGTTTCCCGGGCGGCGCACCA126130.15351337877676624No Hit
CCCCCCACTGCTAAATTTGACTGTA120930.14718443586358793No Hit
CGCCTGTCTGAGCGTCGCT120930.14718443586358793No Hit
TATTGCACTTGTCCCGGCCTGTAAT120900.14714792273139649No Hit
TATTGCACTTGTCCCGGCCTGTTT115470.14053904580475066No Hit
TGTAAACATCCCCGACTGGAAGC110820.13487951031508155No Hit
AGCAGCATTGTACAGGGCTATGT108960.13261569611921392No Hit
CCCCCCACTGCTAAATTTGACTGA107850.1312647102281316No Hit
CCCACCACTGCTAAATTTGACTG104870.12763773909711787No Hit
CCACCCACTGCTAAATTTGACTG103730.12625024007384417No Hit
CACCCCACTGCTAAATTTGACTG102100.12426635989144401No Hit
CAACGGAATCCCAAAAGCAGCT99910.12160090124147084No Hit
TATTGCACTTGTCCCGGCCTGTAAA99570.12118708574330149No Hit
TTATAAAGCAATGAGACTGATT91300.11112163230253516No Hit
GACCCGCGGGCGCTCTCCAGTCCT90990.11074432993655721No Hit
CCCCCAACTGCTAAATTTGACTG85640.1042328213624218No Hit
ACTGGACTTGGAGTCAGAAGGCT85260.10377032168799724No Hit
TCAGTGCACTACAGAACTTTGT84570.10293051964759471No Hit
TGAGGTAGTAGGTTGTGTGGTT83040.10106834990583265No Hit
CAAAGTGCTGTTCGTGCAGGTAG83020.10104400781770502No Hit
CCCATAAAGTAGAAAGCACTA82940.10094663946519458No Hit
ACCCCCACTGCTAAATTTGACTG82190.10003381116040924No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)