FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6677686
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6608619.89655698096616No Hit
CAACGGAATCCCAAAAGCAGCTG5462128.17965984025005No Hit
CCCCCACTGCTAAATTTGACTG2788834.176341924433104No Hit
TTCAAGTAATCCAGGATAGGCT2518303.7712165561543323No Hit
AACATTCAACGCTGTCGGTGAGT1869892.8002065386123274No Hit
AAGCTGCCAGTTGAAGAACTGT1862552.789214707010782No Hit
TAGCAGCACGTAAATATTGGCG1505472.2544785723677334No Hit
AACATTCAACGCTGTCGGTGAG1277451.913012980843963No Hit
GCCTGTCTGAGCGTCGCT1207901.8088601350827218No Hit
TAGCTTATCAGACTGATGTTGACT925901.3865581580206077No Hit
TAGCTTATCAGACTGATGTTGAC900311.348236499889333No Hit
TAGCTTATCAGACTGATGTTGA720521.0789965266411148No Hit
TATTGCACTTGTCCCGGCCTGTAA683721.0238876161592505No Hit
TATTGCACTTGTCCCGGCCTGTAT618920.9268480129194454No Hit
TGAGGTAGTAGGTTGTATAGTT597780.8951903398872004No Hit
GGAATACCGGGTGCTGTAGGCTTT543160.8133955385143896No Hit
TATTGCACTTGTCCCGGCCTGT507420.7598740042583614No Hit
CCCATAAAGTAGAAAGCACTAC417230.6248122478355527No Hit
CATAAAGTAGAAAGCACTACT412010.6169951686856795No Hit
TCCTGTCTGAGCGTCGCT380700.5701076690338539No Hit
TGTAAACATCCTTGACTGGAAGCT375520.562350490873635No Hit
GGAATACCGGGTGCTGTAGGCTT356510.5338825455404762No Hit
TGAGGTAGTAGATTGTATAGTT351350.5261553178750843No Hit
CAACGGAATCCCAAAAGCAGCTGT318160.4764524717095113No Hit
CCCATAAAGTAGAAAGCACT317220.4750447984526377No Hit
TATTGCACTTGTCCCGGCCTGTA284450.4259709126784338No Hit
CAACGGAATCCCAAAAGCAGCT283000.42379950180346904No Hit
TTCAAGTAATTCAGGATAGGTT261940.39226163075053244No Hit
AGCAGCATTGTACAGGGCTATGA258500.3871101456402712No Hit
CTCCCACTGCTTCACTTGACTAG257070.3849686852601335No Hit
CGCCTGTCTGAGCGTCGCT238820.3576388587304045No Hit
ACTGGACTTGGAGTCAGAAGGC220010.3294704183455167No Hit
TGTAAACATCCCCGACTGGAAGCT217600.32586138371885115No Hit
AACCGGGCGGAAACACCA215000.32196781939132807No Hit
TCGTACGACTCTTAGCGG211330.31647190359055516No Hit
CACCCGGCTGTGTGCACATGTGC209230.31332710163370964No Hit
TCGTTTCCCGGCCAATGCACCA191460.28671608697983103No Hit
CATTGCACTTGTCTCGGTCTGA190230.28487413154796437No Hit
TTCTCACTACTGCACTTGACTAG183960.27548465141966844No Hit
CCCCCACTGCTAAATTTGACTGGCTT175640.26302524557159473No Hit
TGTAAACATCCTACACTCTCAGCT172540.2583829188733942No Hit
CAACACCAGTCGATGGGCTGT171690.2571100228432424No Hit
TTAATGCTAATCGTGATAGGGGTT170900.25592697829757194No Hit
TGAATACCGGGTGCTGTAGGCTTT170610.25549269612257897No Hit
ACCCCCCACTGCTAAATTTGACTG154370.23117289432297355No Hit
TGAGGTAGTAGTTTGTACAGTT145460.21782994887750037No Hit
ACCGGGTGCTGTAGGCTT138850.20793131033714374No Hit
AACGGAATCCCAAAAGCAGCTG132750.19879640941487814No Hit
TTCAAGTAATCCAGGATAGGC131880.1974935628898993No Hit
CCCCCCACTGCTAAATTTGACTGGCT124300.18614232535042827No Hit
CTTCTCACTACTGCACTTGACTAG119980.17967301846777461No Hit
TGAATACCGGGTGCTGTAGGCTT112750.16884591458777787No Hit
ATCCCGGACGAGCCCCCA111850.16749814232055835No Hit
ACCGGGTGCTGTAGGCTTA107020.16026509781981363No Hit
TAGCTTATCAGACTGATGTTGACA105890.15857289486208248No Hit
CCCCCCACTGCTAAATTTGACTGG104940.1571502463577952No Hit
CTCCCACTGCTTCACTTGACTAGCCT103430.15488898399834913No Hit
AACATTCAACGCTGTCGGTGAGTT103380.15481410776128138No Hit
CTACGCCTGTCTGAGCGTCGCT102710.15381076618457354No Hit
ACCGGGTGCTGTAGGCTTT102170.15300210282424181No Hit
TTCAAGTAATCCAGGATAGGCTT99530.1490486375070646No Hit
CAAAGAATTCTCCTTTTGGGCT96460.14445123655110467No Hit
CCCCCCACTGCTAAATTTGACTGT95450.1429387365623361No Hit
TAGCAGCACGTAAATATTGGC94830.142010271222696No Hit
AGCTCGGTCTGAGGCCCCTCAGT90980.1362448009684792No Hit
TTCACAGTGGCTAAGTTCTGC88600.13268069208405428No Hit
TCCCACTGCTTCACTTGACTAG86830.1300300732918559No Hit
TGTAAACATCCTACACTCAGCT82500.12354579116178868No Hit
GGAATACCGGGTGCTGTAGGCTTA81460.12198836543077947No Hit
TATTGCACTTGTCCCGGCCTGTT80470.120505815936838No Hit
TGAGGTAGTAGTTTGTGCTGTT80420.12043093969977024No Hit
TATTGCACTTGTCCCGGCCTGTAG80270.120206310988567No Hit
CACTAGATTGTGAGCTCCTGGA79360.11884356347393393No Hit
TAGCAGCACATCATGGTTTACA79170.11855903377307649No Hit
TTCACAGTGGCTAAGTTCCG78130.11700160804206726No Hit
TTCACAGTGGCTAAGTTCCGC77230.11565383577484775No Hit
TGAGGTAGTAGGTTGTGTGGTT76340.11432103875504179No Hit
ACCACAGGGTAGAACCACGGAC72500.10857054374823853No Hit
CCCATAAAGTAGAAAGCACTA72420.10845074176893013No Hit
TACGCCTGTCTGAGCGTCGCT72280.10824108830514044No Hit
ATCACATTGCCAGGGATTTCC70850.10609962792500277No Hit
CAACGGAATCCCAAAAGCAGCTGA68980.1032992566586689No Hit
ACGCCTGTCTGAGCGTCGCT68660.10282004874143527No Hit
CCCCCCACTGCTAAATTTGACTGTA68590.10271522200954043No Hit
AATGCACCCGGGCAAGGATTCT68010.10184665765955452No Hit
AACATTCAACGCTGTCGGTGA67380.10090321707250086No Hit
CACTAGATTGTGAGCTCCTGGAA67320.10081336558801957No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)