FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7721036
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG82421910.674979368053718No Hit
CAACGGAATCCCAAAAGCAGCTG7311569.469661843307039No Hit
CCCCCACTGCTAAATTTGACTG2829773.665013347949679No Hit
TTCAAGTAATCCAGGATAGGCT2461013.187409047179679No Hit
AAGCTGCCAGTTGAAGAACTGT2336823.0265627566041653No Hit
AACATTCAACGCTGTCGGTGAGT2179672.8230278941841482No Hit
TAGCAGCACGTAAATATTGGCG1749692.2661337157345205No Hit
AACATTCAACGCTGTCGGTGAG1495011.9362816078049632No Hit
TAGCTTATCAGACTGATGTTGACT1270031.6448958404027645No Hit
TATTGCACTTGTCCCGGCCTGTAA1268711.6431862252682152No Hit
TAGCTTATCAGACTGATGTTGAC1177321.5248212804602905No Hit
TATTGCACTTGTCCCGGCCTGTAT1154801.495654210134495No Hit
TGAGGTAGTAGGTTGTATAGTT1001781.2974683708248478No Hit
TAGCTTATCAGACTGATGTTGA971301.2579918031725277No Hit
TATTGCACTTGTCCCGGCCTGT765620.9916026812982093No Hit
TGAGGTAGTAGATTGTATAGTT646860.8377891257080008No Hit
TCGTACGACTCTTAGCGG624800.809217830353336No Hit
TGTAAACATCCTTGACTGGAAGCT608900.7886247389599012No Hit
TATTGCACTTGTCCCGGCCTGTA495880.642245418879021No Hit
AGCAGCATTGTACAGGGCTATGA473460.6132078648512972No Hit
TTCAAGTAATTCAGGATAGGTT465860.6033646261978315No Hit
CCCATAAAGTAGAAAGCACTAC462290.598740894356664No Hit
CAACGGAATCCCAAAAGCAGCTGT440460.5704674864875646No Hit
TGTAAACATCCCCGACTGGAAGCT406970.5270924782632797No Hit
CATAAAGTAGAAAGCACTACT401870.5204871470616119No Hit
ACTGGACTTGGAGTCAGAAGGC397820.5152417369896993No Hit
CACCCGGCTGTGTGCACATGTGC353870.4583193239870919No Hit
CAACGGAATCCCAAAAGCAGCT352410.45642838603524194No Hit
TTAATGCTAATCGTGATAGGGGTT293380.37997491528338945No Hit
CCCATAAAGTAGAAAGCACT293240.37979359246608874No Hit
TGAGGTAGTAGTTTGTACAGTT276900.35863062936113754No Hit
CGCCTGTCTGAGCGTCGCT269140.3485801646307568No Hit
CATTGCACTTGTCTCGGTCTGA247600.32068235402606593No Hit
CCCCCACTGCTAAATTTGACTGGCTT215660.2793148484218957No Hit
ACCCCCCACTGCTAAATTTGACTG211540.2739787769413327No Hit
TGTAAACATCCTACACTCTCAGCT204100.26434276436478216No Hit
TTCTCACTACTGCACTTGACTAG203220.26320302094174924No Hit
TCGTTTCCCGGCCAATGCACCA201010.2603407107543599No Hit
AACGGAATCCCAAAAGCAGCTG199430.25829435324482364No Hit
CTCCCACTGCTTCACTTGACTAG198230.25674015766796066No Hit
CAACACCAGTCGATGGGCTGT174960.22660171510662558No Hit
TAGCTTATCAGACTGATGTTGACA174650.22620021458260264No Hit
CCCCCCACTGCTAAATTTGACTGGCT168600.21836447854925167No Hit
AGCTCGGTCTGAGGCCCCTCAGT161320.20893569204961615No Hit
TGAGGTAGTAGTTTGTGCTGTT157390.20384570153538983No Hit
TGAGAACTGAATTCCATGGGTT155790.20177344076623915No Hit
CTTCTCACTACTGCACTTGACTAG153540.19885932405962103No Hit
CACTAGATTGTGAGCTCCTGGA147830.19146394343971457No Hit
CCCCCCACTGCTAAATTTGACTGT146630.18990974786285156No Hit
TATTGCACTTGTCCCGGCCTGTAG146610.18988384460323718No Hit
ACCGGGTGCTGTAGGCTT145530.18848506858406047No Hit
CAAAGAATTCTCCTTTTGGGCT144010.18651642085336734No Hit
AACATTCAACGCTGTCGGTGAGTT143500.18585588773320055No Hit
GGAATACCGGGTGCTGTAGGCTTT139840.1811155912237684No Hit
CACTAGATTGTGAGCTCCTGGAA138340.17917284675268966No Hit
TATTGCACTTGTCCCGGCCTGTT137330.1778647321421633No Hit
ATCCCGGACGAGCCCCCA133900.17342232311829656No Hit
TCGTTTCCCGGGCGGCGCACCA132490.17159614331548254No Hit
TTCAAGTAATCCAGGATAGGCTT132380.17145367538760342No Hit
TTCAAGTAATCCAGGATAGGC131220.1699512863299692No Hit
ACCGGGTGCTGTAGGCTTA128030.16581971642147506No Hit
CCCCCCACTGCTAAATTTGACTGG122790.15903306240250661No Hit
ACCGGGTGCTGTAGGCTTT116800.15127503614799878No Hit
TGTCAGTTTGTCAAATACCCCAA114710.14856814551829572No Hit
CAACGGAATCCCAAAAGCAGCTGA114300.14803712869620086No Hit
TGAGGTAGTAGGTTGTGTGGTT112800.1460943842251221No Hit
CCCCCCACTGCTAAATTTGACTGTA110980.14373718760021323No Hit
CTCCCACTGCTTCACTTGACTAGCCT105910.13717071128796704No Hit
TGTAAACATCCCCGACTGGAAGC102400.13262468922564277No Hit
AGCAGCATTGTACAGGGCTATGT101550.13152380069203148No Hit
GGAATACCGGGTGCTGTAGGCTT99930.12942563666326642No Hit
TGTCAGTTTGTCAAATACCCCA99710.12914070080750822No Hit
CAAAGAATTCTCCTTTTGGGCTT98530.1276124084902596No Hit
TATTGCACTTGTCCCGGCCTGTAAT96880.12547538957207297No Hit
TATTGCACTTGTCCCGGCCTGTTT95390.12354559673080141No Hit
CTACGCCTGTCTGAGCGTCGCT94500.12239290167796134No Hit
CGCGACCTCAGATCAGACGTGGCG93160.12065738328379766No Hit
TAGCAGCACGTAAATATTGGC92260.11949173660115041No Hit
CCCATAAAGTAGAAAGCACTA90460.11716044323585591No Hit
TGAGGTAGTAGGTTGTATAGT89390.1157746188464864No Hit
TACGCCTGTCTGAGCGTCGCT89240.11558034439937853No Hit
TATTGCACTTGTCCCGGCCTGTAAA89220.11555444113976415No Hit
ACTGGACTTGGAGTCAGAAGGCT86950.11261442117353163No Hit
ACCACAGGGTAGAACCACGGAC85170.11030903106785152No Hit
GACCCGCGGGCGCTCTCCAGTCCT83690.10839218985638714No Hit
GAATACCGGGTGCTGTAGGCTTT83510.10815906051985769No Hit
CGCGACCTCAGATCAGACGTGG83200.10775755999583476No Hit
TTCACAGTGGCTAAGTTCTGC82180.10643649375550121No Hit
TTATAAAGCAATGAGACTGATT81800.10594433182282792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)