FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21592659
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG335653915.544815485670386No Hit
CAACGGAATCCCAAAAGCAGCTG15122957.003746041652397No Hit
CCCCCACTGCTAAATTTGACTG12091695.599907820523633No Hit
TAGCAGCACGTAAATATTGGCG6815463.1563782857868503No Hit
TTCAAGTAATCCAGGATAGGCT6454262.9890992119127153No Hit
AACATTCAACGCTGTCGGTGAGT5982602.770663863121258No Hit
AAGCTGCCAGTTGAAGAACTGT5499092.54674053806898No Hit
AACATTCAACGCTGTCGGTGAG4291511.987485654267962No Hit
TAGCTTATCAGACTGATGTTGAC3452651.5989925094449926No Hit
TAGCTTATCAGACTGATGTTGA3237141.4991854407555827No Hit
TAGCTTATCAGACTGATGTTGACT2743671.2706494369220576No Hit
TCGTACGACTCTTAGCGG2388581.1062000284448525No Hit
TGAGGTAGTAGGTTGTATAGTT2267711.0502226705844797No Hit
TATTGCACTTGTCCCGGCCTGT2136050.9892482440444227No Hit
TATTGCACTTGTCCCGGCCTGTAT2110920.9776100294086059No Hit
TATTGCACTTGTCCCGGCCTGTAA2003960.9280746757497537No Hit
TGAGGTAGTAGATTGTATAGTT1576700.7302018709228909No Hit
TGTAAACATCCTTGACTGGAAGCT1424170.6595621224787555No Hit
TATTGCACTTGTCCCGGCCTGTA1337180.6192752824003751No Hit
CATAAAGTAGAAAGCACTACT1270800.5885333529325869No Hit
TTCAAGTAATTCAGGATAGGTT1218180.564163959612385No Hit
AGCAGCATTGTACAGGGCTATGA1080720.5005034349868629No Hit
CCCATAAAGTAGAAAGCACT992660.4597210561237502No Hit
CTCCCACTGCTTCACTTGACTAG990580.4587577657758593No Hit
CGCCTGTCTGAGCGTCGCT980940.4542932855096726No Hit
CCCATAAAGTAGAAAGCACTAC948360.43920482419511186No Hit
CATTGCACTTGTCTCGGTCTGA875620.4055174492405034No Hit
TGTAAACATCCCCGACTGGAAGCT829430.38412591983229116No Hit
TTAATGCTAATCGTGATAGGGGTT805140.3728767262985073No Hit
ACTGGACTTGGAGTCAGAAGGC802070.3714549467946491No Hit
ACCGGGTGCTGTAGGCTT768410.3558663154917604No Hit
ACCGGGTGCTGTAGGCTTA708040.3279077393849456No Hit
CAACGGAATCCCAAAAGCAGCTGT699630.3240128971610213No Hit
TGAGGTAGTAGTTTGTACAGTT684920.3172003966718504No Hit
ATCCCGGACGAGCCCCCA661710.3064513731263945No Hit
CACCCGGCTGTGTGCACATGTGC636830.2949289385804685No Hit
ACCGGGTGCTGTAGGCTTT635330.2942342580411241No Hit
TGTAAACATCCTACACTCTCAGCT621240.2877088921748822No Hit
TTCTCACTACTGCACTTGACTAG619650.28697253080317714No Hit
CAACACCAGTCGATGGGCTGT545690.2527201490099019No Hit
AGCTCGGTCTGAGGCCCCTCAGT477280.22103808521220106No Hit
AACGGAATCCCAAAAGCAGCTG456220.21128477043980548No Hit
CCCCCCACTGCTAAATTTGACTGG453060.20982131010358662No Hit
CACTAGATTGTGAGCTCCTGGA452180.2094137641871712No Hit
GACCCGCGGGCGCTCTCCAGTCCT452170.20940913298357558No Hit
TGAGGTAGTAGTTTGTGCTGTT440260.20389336950118095No Hit
CTTCTCACTACTGCACTTGACTAG437940.2028189302669949No Hit
CCCCCCACTGCTAAATTTGACTGT436920.2023465475002407No Hit
GGAATACCGGGTGCTGTAGGCTTT410080.18991639704957133No Hit
ACCCCCCACTGCTAAATTTGACTG408420.18914761725269685No Hit
CTACGCCTGTCTGAGCGTCGCT403970.18708673165264175No Hit
AACATTCAACGCTGTCGGTGAGTT400070.1852805622503463No Hit
CCCCCCACTGCTAAATTTGACTGGCT389920.18057989060078242No Hit
TATTGCACTTGTCCCGGCCTGTT386530.17900991258186405No Hit
TTCAAGTAATCCAGGATAGGC380390.1761663535741476No Hit
TAGCTTATCAGACTGATGTTGACA374920.17363308520733828No Hit
GGAATACCGGGTGCTGTAGGCTT368830.17081268221759996No Hit
TTCACAGTGGCTAAGTTCCG368360.17059501564860538No Hit
TGAGAACTGAATTCCATGGGTT364890.168987988000922No Hit
GCCTGTCTGAGCGTCGCT356730.16520892586688837No Hit
TACGCCTGTCTGAGCGTCGCT348630.16145765095442854No Hit
CCCCCCACTGCTAAATTTGACTGA348390.16134650206813345No Hit
CCCCCACTGCTAAATTTGACTGGCTT326200.15106986128943176No Hit
CACTAGATTGTGAGCTCCTGGAA325360.15068084018739888No Hit
TTCACCACCTTCTCCACCCAGC324700.15037518075008732No Hit
CTCCCACTGCTTCACTTGACTAGCCT317850.14720280628708118No Hit
TATTGCACTTGTCCCGGCCTGTAG315720.14621635992121212No Hit
TGTCAGTTTGTCAAATACCCCA313620.14524380716612995No Hit
TAGCAGCACGTAAATATTGGC312560.1447528995849932No Hit
TGAGGTAGTAGGTTGTGTGGTT311340.14418789274632643No Hit
CCCATAAAGTAGAAAGCACTA302630.1401541144145332No Hit
TCGTTTCCCGGGCGGCGCACCA302080.13989939821677358No Hit
TGTCAGTTTGTCAAATACCCCAA298330.13816269686841254No Hit
CTCGTACGACTCTTAGCGG285670.1322995931163457No Hit
ATCCCACCGCTGCCACCA283730.1314011396187936No Hit
TTCAAGTAATCCAGGATAGGCTT276900.1282380275629787No Hit
CTCCCACTGCTTCACTTGACTA269770.1249359793992949No Hit
CAAAGAATTCTCCTTTTGGGCT266930.12362071757813617No Hit
CAACGGAATCCCAAAAGCAGCT262120.12139310864863842No Hit
TCAGTGCACTACAGAACTTTGT259700.12027235737849609No Hit
TCCCACTGCTTCACTTGACTAG255850.11848934399417876No Hit
CCCCCACAACCGCGCTTGACTAG254770.1179891740058508No Hit
AATGACACGATCACTCCCGTTG247790.11475659389610146No Hit
AGCAGCATTGTACAGGGCTATG243960.11298284291897538No Hit
TGAGGTAGTAGGTTGTATAGT243250.11265402746368569No Hit
GAATACCGGGTGCTGTAGGCTTT242250.11219090710412274No Hit
ACTGGACTTGGAGTCAGAAGG240100.11119519833106242No Hit
TTCACAGTGGCTAAGTTCTG238240.11033379446227536No Hit
AACATTCAACGCTGTCGGTGA229720.10638800899879909No Hit
CGCGACCTCAGATCAGACGTGG225560.10446142830301723No Hit
CCCCCCACTGCTAAATTTGACTGTA220220.10198836558295112No Hit
AGCAGCATTGTACAGGGCTATGT219090.101465039576645No Hit
TGAGGGGCAGAGAGCGAGACTTT218900.10137704670832805No Hit
CAAAGTGCTGTTCGTGCAGGT218810.10133536587596738No Hit
AGAGGTAGTAGGTTGCATAGTT217120.100552692468306No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)