FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9598110
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6831397.117432494522359No Hit
CCCCCCACTGCTAAATTTGACTG6827657.113535894045807No Hit
TAGCAGCACGTAAATATTGGCG3579443.729317542724557No Hit
TTCAAGTAATCCAGGATAGGCT3439173.5831741874181477No Hit
CCCCCACTGCTAAATTTGACTG3344743.484790234744132No Hit
AAGCTGCCAGTTGAAGAACTGT2855222.974773158465573No Hit
AACATTCAACGCTGTCGGTGAGT2368892.46807965318172No Hit
TAGCTTATCAGACTGATGTTGA2356772.455452167145407No Hit
TAGCTTATCAGACTGATGTTGAC2062032.1483708771831123No Hit
TAGCTTATCAGACTGATGTTGACT1920882.0013106747057496No Hit
AACATTCAACGCTGTCGGTGAG1868681.9469249675196472No Hit
CATAAAGTAGAAAGCACTACT1186711.23639966618428No Hit
TATTGCACTTGTCCCGGCCTGTAT1134661.182170239765954No Hit
TGAGGTAGTAGGTTGTATAGTT1129931.1772421862220792No Hit
TATTGCACTTGTCCCGGCCTGT1088611.1341920440586741No Hit
TATTGCACTTGTCCCGGCCTGTAA1066581.1112396086312826No Hit
TGAGGTAGTAGATTGTATAGTT851440.887091312768868No Hit
TCGTACGACTCTTAGCGG850090.8856847858588826No Hit
TGTAAACATCCTTGACTGGAAGCT844610.879975328476127No Hit
TATTGCACTTGTCCCGGCCTGTA653730.6811028421220428No Hit
TTCAAGTAATTCAGGATAGGTT633220.6597340518081164No Hit
GCCTGTCTGAGCGTCGCT609720.6352500648565187No Hit
AGCAGCATTGTACAGGGCTATGA595430.6203617170463769No Hit
CCCATAAAGTAGAAAGCACT555050.5782909343610356No Hit
CGCCTGTCTGAGCGTCGCT483340.5035783086461814No Hit
CCCATAAAGTAGAAAGCACTAC482020.5022030378897512No Hit
CATTGCACTTGTCTCGGTCTGA472140.49190934465222835No Hit
TGTAAACATCCCCGACTGGAAGCT441870.46037188571500015No Hit
CAACACCAGTCGATGGGCTGT424800.4425871343420736No Hit
ACTGGACTTGGAGTCAGAAGGC376450.3922126335288927No Hit
TGAGGTAGTAGTTTGTACAGTT360130.37520928599484693No Hit
TTCTCACTACTGCACTTGACTAG347780.3623421694479434No Hit
CAACGGAATCCCAAAAGCAGCTGT331230.3450991914033075No Hit
ACCGGGTGCTGTAGGCTT303550.3162601803896809No Hit
CTCCCACTGCTTCACTTGACTAG278320.28997375524973146No Hit
CACCCGGCTGTGTGCACATGTGC278110.28975496217484487No Hit
ATCCCGGACGAGCCCCCA275460.28699400194413277No Hit
TGTAAACATCCTACACTCTCAGCT261340.2722827723374706No Hit
TTCACAGTGGCTAAGTTCCG252610.26318723165289837No Hit
TGTCAGTTTGTCAAATACCCCAA244550.2547897450643929No Hit
ACCGGGTGCTGTAGGCTTA243620.25382080430418075No Hit
TAGCTTATCAGACTGATGTTGACA223540.2329000188578793No Hit
ACCGGGTGCTGTAGGCTTT214350.22332521715212683No Hit
GGAATACCGGGTGCTGTAGGCTTT208990.21774078438359218No Hit
CTTCTCACTACTGCACTTGACTAG206920.21558410978828124No Hit
TTCACAGTGGCTAAGTTCTG204720.21329199186089762No Hit
TTCAAGTAATCCAGGATAGGC199310.20765546550310424No Hit
TATTGCACTTGTCCCGGCCTGTT187310.19515300408101177No Hit
AGCTCGGTCTGAGGCCCCTCAGT186790.1946112307527211No Hit
TAGCAGCACGTAAATATTGGC185840.19362145255680543No Hit
TGTCAGTTTGTCAAATACCCCA185190.1929442358964421No Hit
TGAGGTAGTAGTTTGTGCTGTT182180.18980820182306726No Hit
TTATAAAGCAATGAGACTGATT181460.1890580541377417No Hit
AACGGAATCCCAAAAGCAGCTG180830.18840167491308185No Hit
AGAATTACTCTGAGACCT178410.18588034519295984No Hit
CACTAGATTGTGAGCTCCTGGA178340.18580741416799765No Hit
AACATTCAACGCTGTCGGTGAGTT169690.17679522322623933No Hit
TTCAAGTAATCCAGGATAGGCTT165430.1723568494213965No Hit
GGAATACCGGGTGCTGTAGGCTT157420.16401145642214976No Hit
CCCCCACTGCTAAATTTGACTGGCTT154340.1608024913238127No Hit
CACTAGATTGTGAGCTCCTGGAA153890.16033364902048425No Hit
CTACGCCTGTCTGAGCGTCGCT152810.15920842749249592No Hit
CAAAGAATTCTCCTTTTGGGCT152060.15842702365361513No Hit
ATCCCACCGCTGCCACCA151150.15747892032910646No Hit
CCCATAAAGTAGAAAGCACTA150790.15710384648644368No Hit
TAGCTTATCAGACTGATGTTG142980.14896682784423182No Hit
AGCAGCATTGTACAGGGCTATGT142590.1485604978480138No Hit
TCAGTGCACTACAGAACTTTGT142070.14801872451972314No Hit
TATTGCACTTGTCCCGGCCTGTAG140460.1463413109455924No Hit
TTCACAGTGGCTAAGTTCCGC138910.14472640967857214No Hit
ACCCCCCACTGCTAAATTTGACTG135660.14134032637675542No Hit
TGTAAACATCCCCGACTGGAAGC130960.13644352898643586No Hit
AGCAGCATTGTACAGGGCTATG126440.1317342685174477No Hit
TACGCCTGTCTGAGCGTCGCT124860.13008811109687218No Hit
TGTAAACATCCTACACTCAGCT123230.12838986008703795No Hit
TTCACAGTGGCTAAGTTCTGC123060.12821274188355833No Hit
TGATGTAGTAGGTTGTATAGTT120930.12599355498113693No Hit
TGAGGTAGTAGGTTGTATAGT119700.12471205268537243No Hit
TCCTGTCTGAGCGTCGCT118680.12364934346449456No Hit
ACTGGACTTGGAGTCAGAAGG116370.12124261964074176No Hit
CAACGGAATCCCAAAAGCAGCT116270.12113843246222433No Hit
ACCACAGGGTAGAACCACGGAC116220.1210863388729656No Hit
CCCCCCACTGCTAAATTTGACTGG115130.11995069862712555No Hit
CCCCCCACTGCTAAATTTGACTGGCT114730.1195339499130558No Hit
TGTCAGTTTGTCAAATACC112380.11708555121789602No Hit
CAAAGTGCTGTTCGTGCAGGT104330.10869848334724233No Hit
TATTGCACTTGTCCCGGCCTGA103190.10751074951214354No Hit
TGTCAGTTTGTCAAATACCCC100480.10468727697432098No Hit
ACGCCTGTCTGAGCGTCGCT99930.1041142474924751No Hit
AACCGGGCGGAAACACCA99860.10404131646751288No Hit
TGAGGTAGTAGATTGTATAGT99350.10350996185707394No Hit
AATGACACGATCACTCCCGTTG99070.10321823775722512No Hit
TGAGGTAGTAGGTTGTGTGGTT98360.10247850878975132No Hit
TGATGTAGTAGATTGTATAGTT97030.1010928193154694No Hit
TAGCAGCACGTAAATATTGGCGT96850.100905282394138No Hit
TAGTGGGTTATCAGAACT96140.10016555342666422No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)