FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8148730
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG83837610.28842531290152No Hit
CCCCCCACTGCTAAATTTGACTG7447839.139865966843914No Hit
TTCAAGTAATCCAGGATAGGCT3705784.547677981722305No Hit
TAGCAGCACGTAAATATTGGCG3395624.16705425262587No Hit
AAGCTGCCAGTTGAAGAACTGT3085423.786381436125629No Hit
AACATTCAACGCTGTCGGTGAGT3026903.714566564360336No Hit
CCCCCACTGCTAAATTTGACTG2135682.620874663904682No Hit
AACATTCAACGCTGTCGGTGAG1907322.3406346755874843No Hit
TATTGCACTTGTCCCGGCCTGTAA1510521.8536876298515228No Hit
TATTGCACTTGTCCCGGCCTGTAT1422751.7459775940545337No Hit
TAGCTTATCAGACTGATGTTGAC1308241.6054526288145514No Hit
TAGCTTATCAGACTGATGTTGACT1226171.5047375480596363No Hit
TATTGCACTTGTCCCGGCCTGT1172601.4389972425150914No Hit
TGAGGTAGTAGGTTGTATAGTT1124681.3801905327578654No Hit
TGTAAACATCCTTGACTGGAAGCT858541.0535874915477628No Hit
TAGCTTATCAGACTGATGTTGA857011.0517098983522586No Hit
CATAAAGTAGAAAGCACTACT829941.0184899978278823No Hit
TATTGCACTTGTCCCGGCCTGTA722170.886236260128879No Hit
TGAGGTAGTAGATTGTATAGTT694820.8526727477778747No Hit
TTCAAGTAATTCAGGATAGGTT689530.8461809386247918No Hit
AGCAGCATTGTACAGGGCTATGA625090.7671011310965021No Hit
CCCATAAAGTAGAAAGCACTAC546330.6704480330063703No Hit
TGTAAACATCCCCGACTGGAAGCT541120.6640543986608957No Hit
CAACGGAATCCCAAAAGCAGCTGT516540.6338901890233203No Hit
ACTGGACTTGGAGTCAGAAGGC491400.6030387557324884No Hit
CACCCGGCTGTGTGCACATGTGC456370.5600504618511105No Hit
CAACGGAATCCCAAAAGCAGCT433000.531371146178607No Hit
TCGTACGACTCTTAGCGG409000.50191870389619No Hit
CATTGCACTTGTCTCGGTCTGA357100.4382277974604632No Hit
CCCATAAAGTAGAAAGCACT344950.4233174985549895No Hit
TGAGGTAGTAGTTTGTACAGTT311870.3827222156090581No Hit
TGTAAACATCCTACACTCTCAGCT272410.3342974917563841No Hit
TTCTCACTACTGCACTTGACTAG263630.32352280662139987No Hit
CTTCTCACTACTGCACTTGACTAG247490.3037160391864745No Hit
CAAAGAATTCTCCTTTTGGGCT236450.29016791573656264No Hit
TAGCAGCACGTAAATATTGGC223460.27422678135120443No Hit
TCGTTTCCCGGCCAATGCACCA218450.2680785840247499No Hit
CAACACCAGTCGATGGGCTGT213370.26184448374163827No Hit
CACTAGATTGTGAGCTCCTGGA213080.26148860006405905No Hit
AACATTCAACGCTGTCGGTGAGTT205360.2520147311298816No Hit
GCCTGTCTGAGCGTCGCT204410.2508489052895359No Hit
TATTGCACTTGTCCCGGCCTGTT203150.24930265206970903No Hit
CTCCCACTGCTTCACTTGACTAG194500.23868750099708788No Hit
CAAAGAATTCTCCTTTTGGGCTT192450.23617177155213143No Hit
AACGGAATCCCAAAAGCAGCTG191500.23500594571178576No Hit
TGAGGTAGTAGTTTGTGCTGTT187340.22990085571616684No Hit
TTCAAGTAATCCAGGATAGGCTT181690.2229672599288478No Hit
AGCTCGGTCTGAGGCCCCTCAGT171750.21076904008354674No Hit
TATTGCACTTGTCCCGGCCTGTAG169120.20754154328343188No Hit
TGATGTAGTAGGTTGTATAGTT167580.2056516782369768No Hit
CACTAGATTGTGAGCTCCTGGAA166810.20470674571374925No Hit
CGCCTGTCTGAGCGTCGCT163990.20124608374556527No Hit
TAGCTTATCAGACTGATGTTGACA161130.19773633437357724No Hit
TTCAAGTAATCCAGGATAGGC161000.19757680031121413No Hit
TGTAAACATCCCCGACTGGAAGC155620.19097454449957232No Hit
TGTCAGTTTGTCAAATACCCCAA153080.18785749435801652No Hit
TTCACAGTGGCTAAGTTCCGC151580.18601671671536546No Hit
CCCCCCACTGCTAAATTTGACTGGCT144550.1773896054968075No Hit
CCCCCCACTGCTAAATTTGACTGG144400.17720552773254236No Hit
TTCACAGTGGCTAAGTTCTGC141480.1736221472548483No Hit
CCCCCACTGCTAAATTTGACTGGCTT138760.17028420379617437No Hit
TTCACAGTGGCTAAGTTCCG137000.16812435802879713No Hit
AGCAGCATTGTACAGGGCTATGT133360.16365740428263054No Hit
TTATAAAGCAATGAGACTGATT131060.16083487856389891No Hit
TGTCAGTTTGTCAAATACCCCA127120.15599976928920212No Hit
ACTGGACTTGGAGTCAGAAGGCA125840.15442897236747322No Hit
ACCACAGGGTAGAACCACGGAC124050.15223231104724294No Hit
CCCATAAAGTAGAAAGCACTA122630.15048970821219992No Hit
TATTGCACTTGTCCCGGCCTGTTT120660.14807215357485154No Hit
CAACGGAATCCCAAAAGCAGCTGA118310.14518826860136488No Hit
TGAGGTAGTAGGTTGTGTGGTT118070.1448937441785407No Hit
CAAAGTGCTGTTCGTGCAGGTAG115530.1417766940369849No Hit
ACTGGACTTGGAGTCAGAAGGCT113530.1393223238467835No Hit
CAAAGTGCTGTTCGTGCAGGT112780.13840193502545795No Hit
TCGTTTCCCGGGCGGCGCACCA112340.13786197358361363No Hit
ACCCCCCACTGCTAAATTTGACTG109220.13403315608689942No Hit
TAGCAGCACGTAAATATTGGCGT108300.1329041457994068No Hit
ATCCCGGACGAGCCCCCA105100.1289771534950845No Hit
TATTGCACTTGTCCCGGCCTGA104160.12782359950568983No Hit
TGATGTAGTAGATTGTATAGTT103750.12732045361669855No Hit
AGCAGCATTGTACAGGGCTATG101700.1248047241717421No Hit
TATTGCACTTGTCCCGGCCTGTAAT101070.12403159756182866No Hit
TTCACAGTGGCTAAGTTCTG97040.11908604162857278No Hit
TGAGGTAGTAGGTTGTATAGT96720.11869334239814058No Hit
ACTGGACTTGGAGTCAGAAGG95050.11664394328932239No Hit
TGTAAACATCCTACACTCAGCT94010.11536767079041764No Hit
AGAGGTAGTAGGTTGCATAGTT90210.11070436742903494No Hit
CCCCCCACTGCTAAATTTGACTGT88900.10909675495445303No Hit
ACTTGACTTGGAGTCAGAAGGC88690.10883904608448187No Hit
TATTGCACTTGTCCCGGCCTGTAAA87850.10780821060459728No Hit
ATCACATTGCCAGGGATTTCC87150.10694918103802678No Hit
TAGCAGCACATCATGGTTTACA83760.10278902356563538No Hit
AACATTCAACGCTGTCGGTGA83590.10258040209946825No Hit
AACCGGGCGGAAACACCA82550.10130412960056352No Hit
ATGCACCTGGGCAAGGATTCTGA81600.10013830376021786No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)