FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6148598
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4792107.793809255378218No Hit
CCCCCCACTGCTAAATTTGACTG3563795.7961018105265625No Hit
TTCAAGTAATCCAGGATAGGCT3177035.167080365312548No Hit
AAGCTGCCAGTTGAAGAACTGT2453543.9904056176708904No Hit
AACATTCAACGCTGTCGGTGAGT2230023.626875590175191No Hit
TAGCAGCACGTAAATATTGGCG2109963.431611564132181No Hit
CCCCCACTGCTAAATTTGACTG1565982.546889551081401No Hit
AACATTCAACGCTGTCGGTGAG1466022.3843159042110087No Hit
TGAGGTAGTAGGTTGTATAGTT1252672.03732623274444No Hit
CATAAAGTAGAAAGCACTACT1052951.7125042164083584No Hit
TATTGCACTTGTCCCGGCCTGT988071.6069842263228138No Hit
CGCCTGTCTGAGCGTCGCT842801.3707189834170326No Hit
TATTGCACTTGTCCCGGCCTGTAA670901.0911430540750917No Hit
TGAGGTAGTAGATTGTATAGTT634691.032251579953674No Hit
TATTGCACTTGTCCCGGCCTGTAT613720.9981462440705994No Hit
TAGCTTATCAGACTGATGTTGAC582390.9471915386239269No Hit
TAGCTTATCAGACTGATGTTGA582370.9471590108834567No Hit
TCGTACGACTCTTAGCGG548260.8916829495114171No Hit
TAGCTTATCAGACTGATGTTGACT544620.8857629007458286No Hit
CCCATAAAGTAGAAAGCACT419830.6828060640816004No Hit
CAACACCAGTCGATGGGCTGT404920.6585566335610167No Hit
TGTAAACATCCTTGACTGGAAGCT399490.6497253520233394No Hit
TATTGCACTTGTCCCGGCCTGTA396570.6449763019146804No Hit
CCCATAAAGTAGAAAGCACTAC389810.6339819256357303No Hit
TTCAAGTAATTCAGGATAGGTT366620.5962660105604562No Hit
ACTGGACTTGGAGTCAGAAGGC329840.536447495835636No Hit
AGCAGCATTGTACAGGGCTATGA319350.5193866959589812No Hit
ATCCCGGACGAGCCCCCA298480.4854439987782581No Hit
CACCCGGCTGTGTGCACATGTGC286870.4665616454352683No Hit
CATTGCACTTGTCTCGGTCTGA281780.45828333548558553No Hit
CAACGGAATCCCAAAAGCAGCTGT281320.45753519745476934No Hit
TGAGGTAGTAGTTTGTACAGTT281190.4573237671417127No Hit
TCCACCACGTTCCCGTGG273940.445532461221241No Hit
GCCTGTCTGAGCGTCGCT251690.4093453499480695No Hit
TGTAAACATCCCCGACTGGAAGCT245790.3997496665093408No Hit
TGAGGTAGTAGTTTGTGCTGTT228100.3709788800633901No Hit
TTAATGCTAATCGTGATAGGGGTT211480.3439483277325986No Hit
ACGCCTGTCTGAGCGTCGCT181300.29486396736296633No Hit
TTCACAGTGGCTAAGTTCCG176450.2869759902989267No Hit
TTCACAGTGGCTAAGTTCCGC174540.2838695910840162No Hit
TGAGGTAGTAGGTTGTGTGGTT163910.2665810970240695No Hit
TACGCCTGTCTGAGCGTCGCT163520.26594680608489935No Hit
TTCAAGTAATCCAGGATAGGC162190.2637837113436266No Hit
TTCTCACTACTGCACTTGACTAG158390.257603440654276No Hit
CTACGCCTGTCTGAGCGTCGCT154810.2517809751100983No Hit
AACGGAATCCCAAAAGCAGCTG153940.2503660183996417No Hit
TGTAAACATCCTACACTCTCAGCT151080.24571455151239358No Hit
TGAGAACTGAATTCCATGGGTT148480.2414859452512589No Hit
TGAGGTAGTAGGTTGTATAGT136300.2216765513048666No Hit
CAACGGAATCCCAAAAGCAGCT130390.21206460399590282No Hit
TTCACAGTGGCTAAGTTCTGC127350.2071203874444223No Hit
TATTGCACTTGTCCCGGCCTGTT124400.20232254572505798No Hit
AACATTCAACGCTGTCGGTGAGTT119900.195003804119248No Hit
TAGCAGCACGTAAATATTGGC119340.19409302738608053No Hit
TGTCAGTTTGTCAAATACCCCAA119020.19357258353855628No Hit
TTCACAGTGGCTAAGTTCTG118020.19194619651504294No Hit
TTATAAAGCAATGAGACTGATT112650.18321249819877639No Hit
CAAAGTGCTGTTCGTGCAGGT111920.18202523567161163No Hit
CTCCCACTGCTTCACTTGACTAG111660.18160237504549817No Hit
ACCACAGGGTAGAACCACGGAC109980.17887004484599578No Hit
ATCCCACCGCTGCCACCA105280.17122602583548313No Hit
TGAGAACTGAATTCCATAGGCTGT104020.16917677818585636No Hit
TTCAAGTAATCCAGGATAGGCTT103600.16849369563598074No Hit
AGCTCGGTCTGAGGCCCCTCAGT100190.1629477158858003No Hit
CACTAGATTGTGAGCTCCTGGA99100.1611749540301708No Hit
AATGACACGATCACTCCCGTTG97150.15800349933431979No Hit
AACATTCAACGCTGTCGGTGA96180.15642590392151187No Hit
TGTAAACATCCTACACTCAGCT95780.15577534911210653No Hit
CAAAGAATTCTCCTTTTGGGCT95450.15523864139434712No Hit
ACCGGGTGCTGTAGGCTT94730.15406764273741755No Hit
AGAGGTAGTAGGTTGCATAGTT92080.14975771712510721No Hit
TCGTTTCCCGGCCAATGCACCA89190.1450574586271537No Hit
TAGTGGGTTATCAGAACT88950.14466712574151053No Hit
CTTCTCACTACTGCACTTGACTAG87690.14261787809188373No Hit
ACTGGACTTGGAGTCAGAAGG87240.1418860039313027No Hit
CCCATAAAGTAGAAAGCACTA86930.14138182395401358No Hit
TCAGGCTCAGTCCCCTCCCGA86390.14050357496131638No Hit
TGAGGTAGTAAGTTGTATTGTT85220.1386007021438058No Hit
AGCAGCATTGTACAGGGCTATG84270.13705563447146812No Hit
ACTGGACTTGGAGTCAGAAGGCT79990.13009469801083107No Hit
TATTGCACTTGTCCCGGCCTGTAG77800.1265329104293369No Hit
CAAAGAATTCTCCTTTTGGGCTT76670.12469509309276684No Hit
TGTAAACATCCCCGACTGGAAGC76190.12391442732148045No Hit
TCCTGTCTGAGCGTCGCT72790.11838471144153513No Hit
TATTGCACTTGTCCCGGCCTGA72160.11736008761672173No Hit
TCAGGCTCAGTCCCCTCCCGAT71900.11693722699060827No Hit
TATTGCACTTGTCCCGGCCTG71580.116416783143084No Hit
GTCCGGCCGTGTCGGCGG70790.11513193739450849No Hit
TGTCAGTTTGTCAAATACCCCA70200.11417236905063562No Hit
CCCCCCACTGCTAAATTTGACTGG70150.11409104969945995No Hit
TCGACCGGACCTCGACCGGCT69980.11381456390546268No Hit
TAGCAGCACATAATGGTTTGT69700.11335917553887895No Hit
GCGTTCGCGCTTTCCCCTG69480.11300137039370602No Hit
TGAGGTAGTAGATTGTATAGT69440.11293631491276548No Hit
TCCCCGGCACCTCCACCA69310.11272488459970875No Hit
TAGCAGCACATCATGGTTTACA66750.10856133381951462No Hit
TGTAAACATCCCCGACTGGA65430.10641450294847704No Hit
TGAATCCAGCGATCCGAGT64840.10545493460460417No Hit
AACCGGGCGGAAACACCA64610.1050808655891961No Hit
CTCCTGGCTGGCTCGCCA63020.1024949102218099No Hit
ACTGGACTTGGAGTCAGAAGGCA62450.10156786961840732No Hit
CATAAAGTAGAAAGCACTAC62430.10153534187793704No Hit
TGATGTAGTAGGTTGTATAGTT62320.10135643930535057No Hit
TAATGTGAATTGCAGGACA62080.1009661064197074No Hit
TAGCAGCACGTAAATATTGGCGT61720.1003806070912426No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)