FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13516226
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG11600568.582691647801687No Hit
CCCCCCACTGCTAAATTTGACTG7555715.590103332098768No Hit
TTCAAGTAATCCAGGATAGGCT5233313.871872222320047No Hit
GCCTGTCTGAGCGTCGCT5079653.758186641744522No Hit
AAGCTGCCAGTTGAAGAACTGT4840123.580970013375035No Hit
AACATTCAACGCTGTCGGTGAGT4586603.3934028626038066No Hit
TAGCAGCACGTAAATATTGGCG4560063.3737672039517537No Hit
AACATTCAACGCTGTCGGTGAG2987792.210520895403791No Hit
TATTGCACTTGTCCCGGCCTGTAA2489801.8420822498824745No Hit
CCCCCACTGCTAAATTTGACTG2421511.7915577913538883No Hit
TATTGCACTTGTCCCGGCCTGTAT2334291.72702794404296No Hit
GGAATACCGGGTGCTGTAGGCTTT2022521.496364443743394No Hit
TCCTGTCTGAGCGTCGCT2010751.4876563916584409No Hit
TAGCTTATCAGACTGATGTTGACT2001281.4806499980097996No Hit
TAGCTTATCAGACTGATGTTGAC1974991.461199302231259No Hit
TATTGCACTTGTCCCGGCCTGT1834041.3569172341450935No Hit
TGAGGTAGTAGGTTGTATAGTT1724001.2755039757399735No Hit
CATAAAGTAGAAAGCACTACT1648821.2198819404173915No Hit
TGTAAACATCCTTGACTGGAAGCT1435211.061842262773647No Hit
TAGCTTATCAGACTGATGTTGA1382581.0229038786418634No Hit
GGAATACCGGGTGCTGTAGGCTT1352421.0005899575813544No Hit
TATTGCACTTGTCCCGGCCTGTA1146850.8484986859497614No Hit
TGAGGTAGTAGATTGTATAGTT1091700.8076958760529751No Hit
TTCAAGTAATTCAGGATAGGTT1035330.7659904473334495No Hit
AGCAGCATTGTACAGGGCTATGA939400.6950164935093569No Hit
CCCATAAAGTAGAAAGCACTAC916870.6783476393484394No Hit
TGTAAACATCCCCGACTGGAAGCT838320.6202323044909134No Hit
TGAATACCGGGTGCTGTAGGCTTT836060.6185602401143633No Hit
TCGTACGACTCTTAGCGG730730.5406316822462128No Hit
CAACGGAATCCCAAAAGCAGCTGT698190.5165569146298679No Hit
TTAATGCTAATCGTGATAGGGGTT680790.5036834986334203No Hit
ACTGGACTTGGAGTCAGAAGGC638800.47261713439831504No Hit
CACCCGGCTGTGTGCACATGTGC631260.4670386541331878No Hit
CAACGGAATCCCAAAAGCAGCT609090.4506361465101279No Hit
CCCATAAAGTAGAAAGCACT592050.43802907705153793No Hit
TGAATACCGGGTGCTGTAGGCTT565480.4183712228546637No Hit
CATTGCACTTGTCTCGGTCTGA556660.41184573267715413No Hit
TGAGGTAGTAGTTTGTACAGTT493650.365227690037145No Hit
CGCCTGTCTGAGCGTCGCT481940.3565640290418346No Hit
TGTAAACATCCTACACTCTCAGCT459520.33997655854526254No Hit
CAACACCAGTCGATGGGCTGT403760.29872243923710656No Hit
AACCGGGCGGAAACACCA394450.29183442182751307No Hit
ATCCCGGACGAGCCCCCA386580.2860117905693498No Hit
CAAAGAATTCTCCTTTTGGGCT372370.27549850083891764No Hit
TCGTTTCCCGGCCAATGCACCA338530.25046192628030933No Hit
TGATGTAGTAGGTTGTATAGTT337520.24971467627132013No Hit
TGTCAGTTTGTCAAATACCCCAA318850.23590164887743073No Hit
TGAGAACTGAATTCCATAGGCTGT314410.23261670824385447No Hit
TATTGCACTTGTCCCGGCCTGTT311860.23073008693403024No Hit
GGAATACCGGGTGCTGTAGGCTTA308390.22816280224968125No Hit
CACTAGATTGTGAGCTCCTGGA306060.22643894826854774No Hit
AACATTCAACGCTGTCGGTGAGTT305350.2259136537077732No Hit
TGAGGTAGTAGTTTGTGCTGTT297190.2198764655163357No Hit
CAAAGAATTCTCCTTTTGGGCTT292830.21665071300228333No Hit
TAGCAGCACGTAAATATTGGC285800.21144955699912094No Hit
TATTGCACTTGTCCCGGCCTGTAG283130.20947415351001086No Hit
TTCTCACTACTGCACTTGACTAG276860.20483528464232548No Hit
TTCAAGTAATCCAGGATAGGCTT265210.196216014736658No Hit
TTCACAGTGGCTAAGTTCCGC261070.19315302955129635No Hit
TGAGAACTGAATTCCATGGGTT254560.18833659632503927No Hit
AACGGAATCCCAAAAGCAGCTG253160.18730080423337106No Hit
CACTAGATTGTGAGCTCCTGGAA248080.1835423586436036No Hit
TAGCTTATCAGACTGATGTTGACA244840.1811452398028858No Hit
TGTAAACATCCCCGACTGGAAGC242010.17905146007472797No Hit
TTCAAGTAATCCAGGATAGGC239900.17749037342228519No Hit
TTCACAGTGGCTAAGTTCTGC232790.17223002929959888No Hit
TGTCAGTTTGTCAAATACCCCA229460.16976632382441667No Hit
TTATAAAGCAATGAGACTGATT225430.16678472230340036No Hit
CTTCTCACTACTGCACTTGACTAG224440.16605226932429215No Hit
TTCACAGTGGCTAAGTTCCG221130.1636033608789909No Hit
TGATGTAGTAGATTGTATAGTT214740.15887570983201968No Hit
CCCTGTCTGAGCGTCGCT213590.15802488061386366No Hit
AGCAGCATTGTACAGGGCTATGT211790.156693147924576No Hit
GGAATACCGGGTGCTGTAGGCT206110.15249079143837935No Hit
TATTGCACTTGTCCCGGCCTGTTT205950.15237241519933153No Hit
CCCATAAAGTAGAAAGCACTA198310.14671994978479938No Hit
AGCTCGGTCTGAGGCCCCTCAGT187400.13864816998472798No Hit
ACTGGACTTGGAGTCAGAAGGCA184140.13623625411412918No Hit
ACCACAGGGTAGAACCACGGAC178190.1318341377245394No Hit
GGAATACCAGGTGCTGTAGGCTTT175900.13013987780316785No Hit
TATTGCACTTGTCCCGGCCTGA174540.12913367977126158No Hit
CAAAGTGCTGTTCGTGCAGGT170660.1262630559743526No Hit
TTCACAGTGGCTAAGTTCTG170270.1259745138916736No Hit
CAACGGAATCCCAAAAGCAGCTGA169680.12553800151018488No Hit
CAAAGTGCTGTTCGTGCAGGTAG164740.12188313512958425No Hit
TATTGCACTTGTCCCGGCCTGTAAT162960.12056619947017753No Hit
TATTGCACTTGTCCCGGCCTGTAAA162540.12025546184267709No Hit
ACTGGACTTGGAGTCAGAAGGCT161350.11937503856475913No Hit
TAGCAGCACGTAAATATTGGCGT158360.11716288259755347No Hit
TCGTTTCCCGGGCGGCGCACCA158150.11700751378380327No Hit
TGTAAACATCCTACACTCAGCT156870.1160605038714209No Hit
TGAGGTAGTAGGTTGTGTGGTT156560.11583114990826582No Hit
AGCAGCATTGTACAGGGCTATG154310.11416648404665622No Hit
TGAGGTAGTAGGTTGTATAGT149650.11071877608438924No Hit
CTACGCCTGTCTGAGCGTCGCT147650.10923907309629181No Hit
CACCCGTAGAACCGACCTTGCG145890.10793693446676608No Hit
ACTGGACTTGGAGTCAGAAGG140820.10418588739193914No Hit
CCCCCACTGCTAAATTTGACTGGCTT140540.1039787289736055No Hit
CTCCCACTGCTTCACTTGACTAG138920.10278016955324659No Hit
AGCTACATCTGGCTACTGGGTCTC137240.10153721904324477No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)