FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6773078
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG75295511.116880685561277No Hit
CAACGGAATCCCAAAAGCAGCTG6176349.118955960643005No Hit
TTCAAGTAATCCAGGATAGGCT2655603.9208170937939886No Hit
AACATTCAACGCTGTCGGTGAGT2560733.780747837246227No Hit
TAGCAGCACGTAAATATTGGCG2150063.1744208467701096No Hit
CCCCCACTGCTAAATTTGACTG1944372.870733217600624No Hit
AAGCTGCCAGTTGAAGAACTGT1898922.803629310041904No Hit
AACATTCAACGCTGTCGGTGAG1823482.6922471585296965No Hit
TATTGCACTTGTCCCGGCCTGTAA1437372.122181377506652No Hit
TATTGCACTTGTCCCGGCCTGTAT1258011.8573682452793248No Hit
TAGCTTATCAGACTGATGTTGACT1043511.5406732360087982No Hit
TAGCTTATCAGACTGATGTTGAC889301.3129924090642393No Hit
TGAGGTAGTAGGTTGTATAGTT859971.2696886112931227No Hit
GGAATACCGGGTGCTGTAGGCTTT853631.2603280222079238No Hit
TGTAAACATCCTTGACTGGAAGCT780471.1523121393257245No Hit
TTAATGCTAATCGTGATAGGGGTT780321.1520906742842767No Hit
TATTGCACTTGTCCCGGCCTGT675790.9977590690672689No Hit
TATTGCACTTGTCCCGGCCTGTA592660.8750231430968314No Hit
TAGCTTATCAGACTGATGTTGA552660.8159657987107192No Hit
TGTAAACATCCCCGACTGGAAGCT526540.7774013528265878No Hit
TGAGGTAGTAGATTGTATAGTT517000.7633161761905001No Hit
GGAATACCGGGTGCTGTAGGCTT504940.7455103868580872No Hit
AGCAGCATTGTACAGGGCTATGA467440.6901441264961071No Hit
TTCAAGTAATTCAGGATAGGTT421880.6228778112403254No Hit
CATAAAGTAGAAAGCACTACT418410.6177545866148301No Hit
ACTGGACTTGGAGTCAGAAGGC402670.594515521598895No Hit
CAACGGAATCCCAAAAGCAGCTGT368580.5441838998458308No Hit
CCCATAAAGTAGAAAGCACTAC338930.5004076433196251No Hit
CAACGGAATCCCAAAAGCAGCT331310.48915721921407074No Hit
TGAGAACTGAATTCCATAGGCTGT320380.4730197998605656No Hit
CAAAGAATTCTCCTTTTGGGCT308950.45614416370223404No Hit
TCGTACGACTCTTAGCGG288000.42521287958000775No Hit
CACCCGGCTGTGTGCACATGTGC261910.3866927267041661No Hit
TGAGAACTGAATTCCATGGGTT241420.35644060204238015No Hit
TGTAAACATCCTACACTCTCAGCT232990.343994266713007No Hit
TGAGGTAGTAGTTTGTACAGTT229600.338989156776284No Hit
TGAATACCGGGTGCTGTAGGCTTT206070.3042486739411535No Hit
CATTGCACTTGTCTCGGTCTGA203190.2999965451453534No Hit
CCCATAAAGTAGAAAGCACT201610.297663780042102No Hit
TTCTCACTACTGCACTTGACTAG195210.288214604940324No Hit
AACATTCAACGCTGTCGGTGAGTT192850.2847302216215434No Hit
TATTGCACTTGTCCCGGCCTGTAG177960.2627461251738131No Hit
TCGTTTCCCGGCCAATGCACCA171340.2529721346779116No Hit
CACTAGATTGTGAGCTCCTGGAA168490.24876429889040108No Hit
CTTCTCACTACTGCACTTGACTAG163140.24086537907875857No Hit
CAAAGAATTCTCCTTTTGGGCTT161700.23873931468085854No Hit
CCCCCACTGCTAAATTTGACTGGCTT159560.23557974675620152No Hit
TGTCAGTTTGTCAAATACCCCAA157800.23298122360321258No Hit
CACTAGATTGTGAGCTCCTGGA155140.22905391020153612No Hit
TAGCAGCACGTAAATATTGGC146700.21659281053606647No Hit
TAGCTTATCAGACTGATGTTGACA144380.21316748456167195No Hit
AACGGAATCCCAAAAGCAGCTG143750.21223733138759068No Hit
TATTGCACTTGTCCCGGCCTGTT142640.21059849008087608No Hit
AGCTCGGTCTGAGGCCCCTCAGT138450.20441223325643082No Hit
ACTGGACTTGGAGTCAGAAGGCA138260.20413171087059678No Hit
TGAGGTAGTAGTTTGTGCTGTT134590.198713199523171No Hit
TGTAAACATCCCCGACTGGAAGC132000.19488923647417022No Hit
GGAATACCGGGTGCTGTAGGCTTA129900.19178872589389936No Hit
TATTGCACTTGTCCCGGCCTGTAAA123870.18288583122769295No Hit
TGAATACCGGGTGCTGTAGGCTT123180.1818670920370325No Hit
AGCTACATCTGGCTACTGGGTCTC123060.18168992000387418No Hit
TTCACAGTGGCTAAGTTCCG122760.18124698992097832No Hit
TTAATGCTAATCGTGATAGGGGTTT122270.18052353745224844No Hit
TTCAAGTAATCCAGGATAGGC121890.1799624926805804No Hit
ACCCCCCACTGCTAAATTTGACTG118150.17444063098047888No Hit
ACTGGACTTGGAGTCAGAAGGCT116710.17231456658257885No Hit
TGTCAGTTTGTCAAATACCCCA116330.17175352181091078No Hit
CAACACCAGTCGATGGGCTGT113650.16779667973704127No Hit
TATTGCACTTGTCCCGGCCTGTTT112640.16630548179129195No Hit
TTCAAGTAATCCAGGATAGGCTT112000.16536056428111415No Hit
TCGTTTCCCGGGCGGCGCACCA110400.16299827050566965No Hit
CCCCCCACTGCTAAATTTGACTGGCT109220.16125607884627935No Hit
TATTGCACTTGTCCCGGCCTGTAAT107300.15842132631574596No Hit
CCCCCCACTGCTAAATTTGACTGG105480.15573421714617786No Hit
CGCCTGTCTGAGCGTCGCT96910.14308118111145332No Hit
ATGCACCTGGGCAAGGATTCTGA91870.13563995571880316No Hit
TTCACAGTGGCTAAGTTCTG90950.1342816367979226No Hit
TGAGGTAGTAGGTTGTGTGGTT90540.13367629901796493No Hit
CAACGGAATCCCAAAAGCAGCTGA90520.1336467703457719No Hit
CCCATAAAGTAGAAAGCACTA88400.13051673109330794No Hit
ACTGGACTTGGAGTCAGAAGG88200.1302214443713774No Hit
AGCAGCATTGTACAGGGCTATGT87600.12933558420558572No Hit
TTAATGCTAATCGTGATAGGGGT85900.12682564706917593No Hit
CTCCCACTGCTTCACTTGACTAG83610.123444614103071No Hit
AGCTACATTGTCTGCTGGGTTT83220.12286880499530642No Hit
CCCCCCACTGCTAAATTTGACTGT83040.12260304694556891No Hit
AACATTCAACGCTGTCGGTGA81120.11976829441503553No Hit
ATCCCGGACGAGCCCCCA80260.11849856151073411No Hit
GGAATACCGGGTGCTGTAGGCT79540.1174355293117841No Hit
TTCACAGTGGCTAAGTTCTGC79380.11719929993423965No Hit
AGCTACATCTGGCTACTGGGTCTCT78500.11590003835774518No Hit
CAAAGTGCTGTTCGTGCAGGTAG73750.10888697871189434No Hit
TAGCAGCACATCATGGTTTACA73630.10870980667873603No Hit
ATCACATTGCCAGGGATTTCC73390.10835546261241935No Hit
TGAGGTAGTAGGTTGTATAGT72800.10748436678272419No Hit
TGAGGGGCAGAGAGCGAGACTTT72360.10683473599447696No Hit
AGCTACATCTGGCTACTGGGTCT72080.10642133458377417No Hit
AACATTCAACGCTGTCGGTGAGA70450.1040147478000401No Hit
GGAATACCAGGTGCTGTAGGCTTT70020.10337988134788939No Hit
TTATAAAGCAATGAGACTGATT69540.10267119321525604No Hit
AGCAGCATTGTACAGGGCTATG68640.10134240296656852No Hit
CCCCCCACTGCTAAATTTGACTGTA68200.1006927721783213No Hit
ACCACAGGGTAGAACCACGGAC68120.10057465748954907No Hit
CAAAGTGCTGTTCGTGCAGGT68030.10044177846468032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)