FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6204295
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG67673110.907460074029363No Hit
CCCCCCACTGCTAAATTTGACTG2726084.39385941513097No Hit
TTCAAGTAATCCAGGATAGGCT2294193.6977448686756516No Hit
AACATTCAACGCTGTCGGTGAGT2157643.477655398397401No Hit
AAGCTGCCAGTTGAAGAACTGT1941673.1295578305029013No Hit
TAGCAGCACGTAAATATTGGCG1920553.0955168959567523No Hit
AACATTCAACGCTGTCGGTGAG1779762.8685934501824946No Hit
CCCCCACTGCTAAATTTGACTG1258932.0291265969783834No Hit
TAGCTTATCAGACTGATGTTGAC873901.4085403740473335No Hit
TGAGGTAGTAGGTTGTATAGTT851311.372130113091012No Hit
CGCCTGTCTGAGCGTCGCT828501.3353652590665015No Hit
TATTGCACTTGTCCCGGCCTGT796741.2841749143133911No Hit
CATAAAGTAGAAAGCACTACT757751.221331351910249No Hit
TAGCTTATCAGACTGATGTTGA745751.2019899118272102No Hit
TAGCTTATCAGACTGATGTTGACT710691.1454806710512637No Hit
TATTGCACTTGTCCCGGCCTGTAA628631.013217456616747No Hit
TGTAAACATCCTTGACTGGAAGCT486840.7846822241689023No Hit
TATTGCACTTGTCCCGGCCTGTAT480930.7751565649280056No Hit
AGCAGCATTGTACAGGGCTATGA454870.7331534042143386No Hit
ACTGGACTTGGAGTCAGAAGGC415490.6696812450084981No Hit
CAACACCAGTCGATGGGCTGT414420.6679566332677604No Hit
TATTGCACTTGTCCCGGCCTGTA394230.6354146603280469No Hit
TCGTACGACTCTTAGCGG390990.6301924715056264No Hit
CCCATAAAGTAGAAAGCACT369310.5952489364222687No Hit
TGAGGTAGTAGATTGTATAGTT336890.542994812464591No Hit
TTCAAGTAATTCAGGATAGGTT333610.537708152175227No Hit
CATTGCACTTGTCTCGGTCTGA321880.5188018944940561No Hit
CACCCGGCTGTGTGCACATGTGC321840.5187374230271127No Hit
TGTAAACATCCCCGACTGGAAGCT301690.48625992155434256No Hit
CAACGGAATCCCAAAAGCAGCTGT276610.4458363117807906No Hit
CAACGGAATCCCAAAAGCAGCT268320.43247460025675766No Hit
CCCATAAAGTAGAAAGCACTAC243630.3926795872859044No Hit
TGAGGTAGTAGTTTGTACAGTT190640.3072710114525502No Hit
AACGGAATCCCAAAAGCAGCTG178570.2878167463023599No Hit
TTCACAGTGGCTAAGTTCCG176800.28496388389011157No Hit
TGAGGTAGTAGTTTGTGCTGTT173880.2802574668032387No Hit
TACGCCTGTCTGAGCGTCGCT173160.27909698039825637No Hit
TTCACAGTGGCTAAGTTCTG172990.2788229766637466No Hit
TGTCAGTTTGTCAAATACCCCAA172870.27862956226291624No Hit
ACGCCTGTCTGAGCGTCGCT161630.26051308005180285No Hit
CTACGCCTGTCTGAGCGTCGCT142080.2290026505831847No Hit
ACCACAGGGTAGAACCACGGAC139370.224634708697765No Hit
AGAATTACTCTGAGACCT135870.2189934553402119No Hit
TAGCAGCACGTAAATATTGGC134730.2171560185323232No Hit
AACATTCAACGCTGTCGGTGAGTT132010.2127719587801676No Hit
ACTGGACTTGGAGTCAGAAGG131640.21217559771094055No Hit
TTCAAGTAATCCAGGATAGGC125050.20155392353200485No Hit
TTCACAGTGGCTAAGTTCTGC124100.20002272619209757No Hit
TGAGGTAGTAGGTTGTGTGGTT123280.19870106111975655No Hit
TCGTTTCCCGGCCAATGCACCA121520.19586431657424413No Hit
GCGTTCGCGCTTTCCCCTG121290.19549360563931922No Hit
TTATAAAGCAATGAGACTGATT119540.19267297896054264No Hit
CACTAGATTGTGAGCTCCTGGA114570.18466239919281724No Hit
TTCTCACTACTGCACTTGACTAG114560.18464628132608135No Hit
AGCAGCATTGTACAGGGCTATG114270.18417886319074125No Hit
GCCTGTCTGAGCGTCGCT113040.18219636558222974No Hit
TATTGCACTTGTCCCGGCCTGTT111440.17961750690449116No Hit
CAAAGTGCTGTTCGTGCAGGT110500.17810242743131977No Hit
TTCACAGTGGCTAAGTTCCGC108520.1749110898176183No Hit
CAAAGAATTCTCCTTTTGGGCT106970.17241282047355905No Hit
ATCCCGGACGAGCCCCCA106790.17212269887231346No Hit
ATCCCACCGCTGCCACCA104570.1685445324569512No Hit
TGTAAACATCCTACACTCTCAGCT99150.1598086486861118No Hit
ACTGGACTTGGAGTCAGAAGGCT98050.15803568334516654No Hit
TGTAAACATCCCCGACTGGAAGC97820.1576649724102416No Hit
TAGCTTATCAGACTGATGTTGACA96350.1552956460000693No Hit
TGTCAGTTTGTCAAATACCCCA94500.15231384065393408No Hit
ACTGGACTTGGAGTCAGAAGGCA93290.15036357877889428No Hit
CCCCCACTGCTAAATTTGACTGGCTT92210.14862284917142077No Hit
TTCAAGTAATCCAGGATAGGCTT92100.14844555263732626No Hit
AATGACACGATCACTCCCGTTG91580.14760742356706122No Hit
TGATGTAGTAGGTTGTATAGTT90680.14615681556083326No Hit
TAATGTGAATTGCAGGACA90070.14517362568994543No Hit
CAAAGTGCTGTTCGTGCAGGTAG88650.14288488861345247No Hit
AGCAGCATTGTACAGGGCTATGT83430.13447136217733038No Hit
TGAGGTAGTAGGTTGTATAGT81190.1308609600284964No Hit
CCCATAAAGTAGAAAGCACTA79210.1276696224147949No Hit
CACTAGATTGTGAGCTCCTGGAA78590.12667031467717121No Hit
CAAAGAATTCTCCTTTTGGGCTT76620.1234950949302056No Hit
CTCCCACTGCTTCACTTGACTAG72570.11696735890217985No Hit
AATGCACCCGGGCAAGGATTCT71490.11522662929470631No Hit
TGTAAACATCCCCGACTGGA70900.11427567515729024No Hit
TATTGCACTTGTCCCGGCCTGTAG70680.11392108208910118No Hit
CAACGGAATCCCAAAAGCAGCTGA68270.11003667620575745No Hit
CTTCTCACTACTGCACTTGACTAG67180.10827982873154807No Hit
ACCGGGTGCTGTAGGCTT65720.10592662018811164No Hit
TCGTTCGCGCTTTCCCCTG62310.10043042763118129No Hit
AACATTCAACGCTGTCGGTGA62200.10025313109708678No Hit
AGAGGTAGTAGGTTGCATAGTT62050.10001136309604879No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)