FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8772069
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG128740314.676161348024053No Hit
CAACGGAATCCCAAAAGCAGCTG6893277.85820312174927No Hit
CCCCCACTGCTAAATTTGACTG3541664.037428342161923No Hit
AACATTCAACGCTGTCGGTGAGT3356943.8268508831838877No Hit
TAGCAGCACGTAAATATTGGCG2952713.3660359944729117No Hit
TTCAAGTAATCCAGGATAGGCT2945973.3583525163789747No Hit
AAGCTGCCAGTTGAAGAACTGT2250662.565711692418288No Hit
AACATTCAACGCTGTCGGTGAG1953272.2266924712972505No Hit
TAGCTTATCAGACTGATGTTGACT1677501.912319659136288No Hit
TAGCTTATCAGACTGATGTTGAC1608851.8340599008056138No Hit
TATTGCACTTGTCCCGGCCTGTAA1477341.684140879420807No Hit
TATTGCACTTGTCCCGGCCTGTAT1351881.5411187486099347No Hit
TAGCTTATCAGACTGATGTTGA1080451.2316934579515961No Hit
TGAGGTAGTAGGTTGTATAGTT1027591.1714340140279336No Hit
GGAATACCGGGTGCTGTAGGCTTT915091.0431860488101496No Hit
TGTAAACATCCTTGACTGGAAGCT864840.9859019576795395No Hit
TATTGCACTTGTCCCGGCCTGT794450.9056586308201634No Hit
TGAGGTAGTAGATTGTATAGTT757130.8631145058252505No Hit
GGAATACCGGGTGCTGTAGGCTT602090.6863717100264487No Hit
TATTGCACTTGTCCCGGCCTGTA573560.6538480260472187No Hit
CATAAAGTAGAAAGCACTACT520700.593588582123556No Hit
ACTGGACTTGGAGTCAGAAGGC502200.5724989167321871No Hit
TTCAAGTAATTCAGGATAGGTT502030.5723051198069692No Hit
CCCATAAAGTAGAAAGCACTAC501460.5716553301165324No Hit
TGTAAACATCCCCGACTGGAAGCT495430.5647812391808592No Hit
AGCAGCATTGTACAGGGCTATGA455220.5189425664572406No Hit
CAACGGAATCCCAAAAGCAGCTGT437560.49881048587283117No Hit
TCGTACGACTCTTAGCGG388520.44290577285700783No Hit
CTCCCACTGCTTCACTTGACTAG382750.43632807721872685No Hit
CTTCTCACTACTGCACTTGACTAG354150.40372459450558357No Hit
CACCCGGCTGTGTGCACATGTGC339910.38749125206379476No Hit
TTCTCACTACTGCACTTGACTAG339880.38745705260640334No Hit
TGAGGTAGTAGTTTGTACAGTT322480.3676213673193861No Hit
CAACGGAATCCCAAAAGCAGCT316840.36119186932980124No Hit
TGTAAACATCCTACACTCTCAGCT283610.3233102703592505No Hit
CATTGCACTTGTCTCGGTCTGA279840.3190125385470634No Hit
CCCATAAAGTAGAAAGCACT262750.2995302476530907No Hit
GGAATACCAGGTGCTGTAGGCTTT256000.2918353697400237No Hit
CCCCCCACTGCTAAATTTGACTGGCT252910.2883128256287086No Hit
AACATTCAACGCTGTCGGTGAGTT236850.2700047161051743No Hit
CCCCCACTGCTAAATTTGACTGGCTT218880.24951924112772025No Hit
CTCCCACTGCTTCACTTGACTAGCCT217270.24768387024771465No Hit
CCCCCCACTGCTAAATTTGACTGG203980.23253351062332045No Hit
TAGCTTATCAGACTGATGTTGACA202900.2313023301572297No Hit
ACCCCCCACTGCTAAATTTGACTG190270.21690435859544654No Hit
TGTCAGTTTGTCAAATACCCCAA188690.2151031871728323No Hit
CAAAGAATTCTCCTTTTGGGCT187570.21382640743021972No Hit
AACGGAATCCCAAAAGCAGCTG186220.21228743184760632No Hit
AGCTCGGTCTGAGGCCCCTCAGT177030.2018109980667047No Hit
TATTGCACTTGTCCCGGCCTGTAG176330.2010130107275718No Hit
GGAATACCAGGTGCTGTAGGCTT174520.1989496434649568No Hit
TAGCAGCACGTAAATATTGGC167270.19068477459536626No Hit
TCAGTGCACTACAGAACTTTGT166550.18986398761797246No Hit
TGAATACCGGGTGCTGTAGGCTTT165280.1884162105884028No Hit
GGAATACCGGGTGCTGTAGGCTTA164890.1879716176423145No Hit
TGAGGTAGTAGTTTGTGCTGTT164650.18769802198318322No Hit
CACTAGATTGTGAGCTCCTGGA164420.1874358261431824No Hit
TCGTTTCCCGGCCAATGCACCA163020.18583985146491666No Hit
CAACACCAGTCGATGGGCTGT153960.17551161533271115No Hit
CGCCTGTCTGAGCGTCGCT152470.17381304228227115No Hit
CAAAGAATTCTCCTTTTGGGCTT149590.1705298943726959No Hit
TATTGCACTTGTCCCGGCCTGTT149590.1705298943726959No Hit
TTCAAGTAATCCAGGATAGGCTT149580.1705184945535654No Hit
CACTAGATTGTGAGCTCCTGGAA146930.16749754248399096No Hit
TGTAAACATCCCCGACTGGAAGC144510.1647387862544173No Hit
TTCAAGTAATCCAGGATAGGC142610.16257282061962805No Hit
GACCCGCGGGCGCTCTCCAGTCCT141170.1609312466648404No Hit
TGTCAGTTTGTCAAATACCCCA135590.15457014759003834No Hit
CCCCCCACTGCTAAATTTGACTGT124870.14234954148217485No Hit
ACTGGACTTGGAGTCAGAAGGCA123820.14115256047347552No Hit
ACTGGACTTGGAGTCAGAAGGCT122160.13926019049781754No Hit
TATTGCACTTGTCCCGGCCTGTAAT116640.1329674903377983No Hit
TATTGCACTTGTCCCGGCCTGTTT116570.132887691603885No Hit
TTCACAGTGGCTAAGTTCTGC107500.12254805565254902No Hit
TCAGTGCACTACAGAACTTTGTC107310.12233145908907009No Hit
TGAATACCGGGTGCTGTAGGCTT106040.12088368205950045No Hit
TCGTTTCCCGGGCGGCGCACCA105530.1203022912838465No Hit
TTATAAAGCAATGAGACTGATT102440.11677974717253137No Hit
TTCACAGTGGCTAAGTTCCGC98410.11218562006295209No Hit
AACATTCAACGCTGTCGGTGA97400.11103423833077465No Hit
TGAGGTAGTAGGTTGTGTGGTT96890.11045284755512069No Hit
CCCATAAAGTAGAAAGCACTA96470.10997405515164096No Hit
CTACGCCTGTCTGAGCGTCGCT95950.10938126455685654No Hit
CAACGGAATCCCAAAAGCAGCTGA95610.10899367070642058No Hit
ATCCCGGACGAGCCCCCA93830.10696450290119697No Hit
AGAGGTAGTAGGTTGCATAGTT91240.10401194974640532No Hit
AGCAGCATTGTACAGGGCTATGT90880.10360155625770842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)