FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6472661
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG96298214.87768322796451No Hit
CAACGGAATCCCAAAAGCAGCTG6324869.771653420440218No Hit
CCCCCACTGCTAAATTTGACTG2504253.86896517521928No Hit
AACATTCAACGCTGTCGGTGAGT2178293.3653701313880027No Hit
TTCAAGTAATCCAGGATAGGCT1813182.801289917701545No Hit
TAGCAGCACGTAAATATTGGCG1774632.741731723629586No Hit
AAGCTGCCAGTTGAAGAACTGT1600902.4733258855979017No Hit
AACATTCAACGCTGTCGGTGAG1324022.0455574608341145No Hit
TATTGCACTTGTCCCGGCCTGTAA1150591.7776151106940408No Hit
TATTGCACTTGTCCCGGCCTGTAT976281.5083131960719092No Hit
TAGCTTATCAGACTGATGTTGAC788611.2183706206767202No Hit
TAGCTTATCAGACTGATGTTGACT782761.2093326067903138No Hit
GGAATACCGGGTGCTGTAGGCTTT670751.036281677659312No Hit
TATTGCACTTGTCCCGGCCTGT658831.0178657587659852No Hit
TGAGGTAGTAGGTTGTATAGTT549530.8490016702558654No Hit
GGAATACCGGGTGCTGTAGGCTT484590.7486719913185628No Hit
TAGCTTATCAGACTGATGTTGA481970.7446241970651637No Hit
TATTGCACTTGTCCCGGCCTGTA452650.6993259804584235No Hit
TGTAAACATCCTTGACTGGAAGCT437520.6759507411248634No Hit
CAACGGAATCCCAAAAGCAGCTGT397700.6144304483117531No Hit
TCGTACGACTCTTAGCGG382160.5904217755263252No Hit
ACTGGACTTGGAGTCAGAAGGC364700.5634467802345898No Hit
AGCAGCATTGTACAGGGCTATGA346730.5356838555271163No Hit
CCCATAAAGTAGAAAGCACTAC339550.5245910453212365No Hit
TGAGGTAGTAGATTGTATAGTT321900.4973225076981476No Hit
TGTAAACATCCCCGACTGGAAGCT298530.46121680094168377No Hit
CACCCGGCTGTGTGCACATGTGC293260.45307486364572463No Hit
CAACGGAATCCCAAAAGCAGCT289340.4470186218620132No Hit
TTCAAGTAATTCAGGATAGGTT287760.44457758563286415No Hit
CATAAAGTAGAAAGCACTACT280000.4325886988365372No Hit
CTCCCACTGCTTCACTTGACTAG215770.333355941242713No Hit
TTAATGCTAATCGTGATAGGGGTT207090.31994569157878033No Hit
CATTGCACTTGTCTCGGTCTGA191470.2958134220222564No Hit
GGAATACCAGGTGCTGTAGGCTTT190900.2949327950281963No Hit
CTTCTCACTACTGCACTTGACTAG189280.29242996041349917No Hit
TGAATACCGGGTGCTGTAGGCTTT182500.2819551340631002No Hit
TCGTTTCCCGGCCAATGCACCA182050.2812599022256843No Hit
CCCCCCACTGCTAAATTTGACTGGCT178640.2759915898577108No Hit
TTCTCACTACTGCACTTGACTAG178060.27509551326726367No Hit
CCCATAAAGTAGAAAGCACT175840.2716657028693454No Hit
CCCCCACTGCTAAATTTGACTGGCTT172540.2665673360616291No Hit
TGTAAACATCCTACACTCTCAGCT172450.2664282896941459No Hit
CCCCCCACTGCTAAATTTGACTGG165850.25623155607871323No Hit
ACCCCCCACTGCTAAATTTGACTG162990.25181297151202575No Hit
AACATTCAACGCTGTCGGTGAGTT156340.24153898991465797No Hit
AACGGAATCCCAAAAGCAGCTG148550.22950375432917006No Hit
CTCCCACTGCTTCACTTGACTAGCCT143730.2220570488706268No Hit
TGAGGTAGTAGTTTGTACAGTT139180.21502748251453302No Hit
AGCTCGGTCTGAGGCCCCTCAGT138420.2138533131891196No Hit
TCGTTTCCCGGGCGGCGCACCA136310.21059344835145855No Hit
TATTGCACTTGTCCCGGCCTGTAG135280.20900213992359556No Hit
CACTAGATTGTGAGCTCCTGGA133200.20578862387509558No Hit
TGAATACCGGGTGCTGTAGGCTT131230.2027450533868528No Hit
GGAATACCAGGTGCTGTAGGCTT130540.2016790312361485No Hit
CGCCTGTCTGAGCGTCGCT122410.1891185093735019No Hit
GGAATACCGGGTGCTGTAGGCTTA120370.18596679171054994No Hit
TATTGCACTTGTCCCGGCCTGTT120300.18585864453584083No Hit
TAGCTTATCAGACTGATGTTGACA113860.17590910446260047No Hit
CACTAGATTGTGAGCTCCTGGAA113850.17589365486621344No Hit
CAAAGAATTCTCCTTTTGGGCT112220.1733753706551293No Hit
ATCCCGGACGAGCCCCCA106210.1640901632265308No Hit
AACCGGGCGGAAACACCA99950.15441871588825679No Hit
TAGCAGCACGTAAATATTGGC99340.1534762905086486No Hit
CAACACCAGTCGATGGGCTGT96320.1488105123997688No Hit
CAACGGAATCCCAAAAGCAGCTGA95390.147373699935776No Hit
CCCCCCACTGCTAAATTTGACTGT94980.14674026648390825No Hit
GACCCGCGGGCGCTCTCCAGTCCT94220.14556609715849478No Hit
TTCAAGTAATCCAGGATAGGCTT92560.1430014641582496No Hit
CAAAGAATTCTCCTTTTGGGCTT91900.14198179079670634No Hit
TGAGGTAGTAGTTTGTGCTGTT87580.13530756515751405No Hit
ACTGGACTTGGAGTCAGAAGGCA86420.1335154119766198No Hit
TGTAAACATCCCCGACTGGAAGC85580.1322176458801102No Hit
TATTGCACTTGTCCCGGCCTGTTT84610.13071903503056934No Hit
TATTGCACTTGTCCCGGCCTGTAAT82670.1277218133314876No Hit
TTCAAGTAATCCAGGATAGGC81370.12571336580117512No Hit
ACTGGACTTGGAGTCAGAAGGCT78840.12180461791525928No Hit
TGAGGTAGTAGGTTGTGTGGTT77640.11995066634881697No Hit
CTACGCCTGTCTGAGCGTCGCT76430.11808126518598765No Hit
ACCACAGGGTAGAACCACGGAC75310.11635091039064151No Hit
GCCTGTCTGAGCGTCGCT75090.11601101927012708No Hit
TGTCAGTTTGTCAAATACCCCAA75030.11591832169180495No Hit
TATTGCACTTGTCCCGGCCTGTAAA73680.11383262617955738No Hit
GGAATACCGGGTGCTGTAGGCT72710.11233401533001651No Hit
AGCAGCATTGTACAGGGCTATGT68660.10607692879327374No Hit
TGTCAGTTTGTCAAATACCCCA67260.10391398529909104No Hit
TCAGTGCACTACAGAACTTTGT67020.10354319498580258No Hit
TATTGCACTTGTCCCGGCCTGA66830.10324965265444921No Hit
TTCACAGTGGCTAAGTTCTGC66650.10297155991948288No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC66310.10244627364232424No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)