FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10013016
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG171916517.16930243594937No Hit
CAACGGAATCCCAAAAGCAGCTG7189927.180573765187233No Hit
CCCCCACTGCTAAATTTGACTG4623484.617469901176628No Hit
TCGTACGACTCTTAGCGG4561964.556029871519231No Hit
AACATTCAACGCTGTCGGTGAGT3151183.1470837557834725No Hit
TTCAAGTAATCCAGGATAGGCT2206642.203771570923286No Hit
AACATTCAACGCTGTCGGTGAG2089102.086384362114272No Hit
TAGCAGCACGTAAATATTGGCG1826831.824455288995843No Hit
AAGCTGCCAGTTGAAGAACTGT1455501.4536079838482232No Hit
TAGCTTATCAGACTGATGTTGAC1392421.3906099820473672No Hit
TATTGCACTTGTCCCGGCCTGTAA1083381.0819717056279547No Hit
TATTGCACTTGTCCCGGCCTGT1002151.0008472971580191No Hit
TATTGCACTTGTCCCGGCCTGTAT940190.9389678394601586No Hit
TAGCTTATCAGACTGATGTTGACT939630.9384085674086609No Hit
TAGCTTATCAGACTGATGTTGA731040.730089715226661No Hit
TATTGCACTTGTCCCGGCCTGTA583580.5828213996661944No Hit
TGAGGTAGTAGGTTGTATAGTT538690.5379897525380964No Hit
TGTAAACATCCTTGACTGGAAGCT494650.4940070004881646No Hit
TTCAAGTAATTCAGGATAGGTT456290.45569686496056727No Hit
CTCCCACTGCTTCACTTGACTAG453780.4531901277297469No Hit
TTAATGCTAATCGTGATAGGGGTT436600.4360324601498689No Hit
AGCAGCATTGTACAGGGCTATGA422700.4221505288716207No Hit
CAACGGAATCCCAAAAGCAGCTGT408410.4078791045575079No Hit
ACTGGACTTGGAGTCAGAAGGC380250.37975570996790575No Hit
TGAGGTAGTAGATTGTATAGTT370050.36956896902991065No Hit
TGTAAACATCCCCGACTGGAAGCT364550.36407611852412897No Hit
CCCCCCACTGCTAAATTTGACTGGCT353290.35283075548865594No Hit
CACCCGGCTGTGTGCACATGTGC343510.34306346858928416No Hit
CTCCCACTGCTTCACTTGACTAGCCT343480.3430335075865254No Hit
CAACGGAATCCCAAAAGCAGCT339350.3389088762067293No Hit
CATAAAGTAGAAAGCACTACT325510.32508686693399874No Hit
CCCATAAAGTAGAAAGCACTAC314370.3139613479095609No Hit
CATTGCACTTGTCTCGGTCTGA303090.3026960108722487No Hit
TCGTTTCCCGGCCAATGCACCA302860.3024663098510978No Hit
CCCCCCACTGCTAAATTTGACTGG282510.28214276297970564No Hit
CCCCCACTGCTAAATTTGACTGGCTT273580.2732243711585001No Hit
TTCTCACTACTGCACTTGACTAG242030.24171538325715247No Hit
CTTCTCACTACTGCACTTGACTAG239590.23927855503276932No Hit
ATCCCGGACGAGCCCCCA238330.23802019291689935No Hit
CGCCTGTCTGAGCGTCGCT231020.23071969524466954No Hit
AACATTCAACGCTGTCGGTGAGTT228160.22786341298166307No Hit
ACCCCCCACTGCTAAATTTGACTG226930.22663501186855187No Hit
CCCATAAAGTAGAAAGCACT218560.21827589209884413No Hit
GCCTGTCTGAGCGTCGCT216490.2162085829084863No Hit
TCGTTTCCCGGGCGGCGCACCA206240.2059719069658932No Hit
TGTAAACATCCTACACTCTCAGCT196300.19604482805180776No Hit
TCGGACGACTCTTAGCGG192820.1925693517317859No Hit
AGCTCGGTCTGAGGCCCCTCAGT189400.18915379741728167No Hit
TAGCTTATCAGACTGATGTTGACA182750.18251244180574566No Hit
GACCCGCGGGCGCTCTCCAGTCCT182120.18188326074781064No Hit
CGCCCCACTGCTAAATTTGACTG181060.18082463865033274No Hit
ACCGGGTGCTGTAGGCTT175440.17521194413351582No Hit
GGAATACCGGGTGCTGTAGGCTTT170650.17042817069302596No Hit
CACTAGATTGTGAGCTCCTGGA165550.1653348002240284No Hit
TACGCCTGTCTGAGCGTCGCT163430.16321755602907256No Hit
TATTGCACTTGTCCCGGCCTGTT162870.16265828397757479No Hit
TGAGGTAGTAGTTTGTACAGTT159230.15902301564283927No Hit
CAAAGAATTCTCCTTTTGGGCT158200.15799435454812016No Hit
CTACGCCTGTCTGAGCGTCGCT155230.15502821527499808No Hit
AACGGAATCCCAAAAGCAGCTG151430.1512331549255489No Hit
CAACACCAGTCGATGGGCTGT150200.15000475381243772No Hit
GGAATACCGGGTGCTGTAGGCTT144860.1446716953213697No Hit
TATTGCACTTGTCCCGGCCTGTAG140120.13993785688547786No Hit
CCCCCCACTGCTAAATTTGACTGT137050.13687184760315974No Hit
TGAGGTAGTAGGTTGTGTGGTT134860.13468469440176667No Hit
TTCACCACCTTCTCCACCCAGC134500.13432516236866093No Hit
CTCGTACGACTCTTAGCGG132960.1327871642270421No Hit
TGAGGTAGTAGTTTGTGCTGTT131050.1308796470513979No Hit
ACCGGGTGCTGTAGGCTTA128910.12874242885460285No Hit
TAGCAGCACGTAAATATTGGC126230.12606591260814923No Hit
CACTAGATTGTGAGCTCCTGGAA125600.12543673155021423No Hit
ACCGGGTGCTGTAGGCTTT120350.12019355606742263No Hit
CCCCCCACTGCTAAATTTGACTGA120010.11985399803615615No Hit
CCCGCCACTGCTAAATTTGACTG119480.11932468698741719No Hit
GCCCCCACTGCTAAATTTGACTG117520.11736723480717498No Hit
ATCCCACCGCTGCCACCA114910.11476062756715859No Hit
TTCAAGTAATCCAGGATAGGC114560.11441108253497248No Hit
TGTCAGTTTGTCAAATACCCCAA112310.11216400732806181No Hit
TGTCAGTTTGTCAAATACCCCA110940.11079578820207617No Hit
CCTCGACACAAGGGTTTGT107560.10742018189125034No Hit
CGTTCGCGCTTTCCCCTG107230.10709061086090345No Hit
TTCAAGTAATCCAGGATAGGCTT106750.1066112348167625No Hit
TGAGGGGCAGAGAGCGAGACTTT104240.10410449758594213No Hit
CTCCCACTGCTTCACTTGACTA103730.10359516053904239No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)