FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7714203
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG96798512.548088247094352No Hit
CAACGGAATCCCAAAAGCAGCTG6073387.872984415888459No Hit
CCCCCACTGCTAAATTTGACTG3290414.265392030777515No Hit
TTCAAGTAATCCAGGATAGGCT2899353.7584569656774653No Hit
AACATTCAACGCTGTCGGTGAGT2682903.477870623835022No Hit
TAGCAGCACGTAAATATTGGCG2131712.7633574071099765No Hit
AAGCTGCCAGTTGAAGAACTGT1803972.3385047035967292No Hit
TAGCTTATCAGACTGATGTTGAC1746982.2646279855482154No Hit
TAGCTTATCAGACTGATGTTGACT1692922.194549456372875No Hit
AACATTCAACGCTGTCGGTGAG1634942.119389391230695No Hit
TAGCTTATCAGACTGATGTTGA1230381.594954138489744No Hit
TATTGCACTTGTCCCGGCCTGTAA1197831.5527592416222389No Hit
TATTGCACTTGTCCCGGCCTGTAT1043581.3528033939475017No Hit
TGAGGTAGTAGGTTGTATAGTT813211.0541724141819964No Hit
TGTAAACATCCTTGACTGGAAGCT679980.8814650068192398No Hit
TGAGGTAGTAGATTGTATAGTT653000.8464905577413506No Hit
TATTGCACTTGTCCCGGCCTGT625570.8109327690754314No Hit
CATAAAGTAGAAAGCACTACT617320.8002382099615476No Hit
TTCAAGTAATTCAGGATAGGTT597560.7746231204960512No Hit
CCCATAAAGTAGAAAGCACTAC519120.6729405487514394No Hit
TCGTACGACTCTTAGCGG508150.658720025905463No Hit
TATTGCACTTGTCCCGGCCTGTA507750.658201501827214No Hit
AGCAGCATTGTACAGGGCTATGA399050.517292583563072No Hit
CTCCCACTGCTTCACTTGACTAG373230.48382185431210456No Hit
CAACGGAATCCCAAAAGCAGCTGT347030.44985852718680075No Hit
TGTAAACATCCCCGACTGGAAGCT335230.4345620668784579No Hit
CCCATAAAGTAGAAAGCACT306810.3977209311188726No Hit
ACTGGACTTGGAGTCAGAAGGC301710.3911097491211989No Hit
CCCCCACTGCTAAATTTGACTGGCTT299330.3880245308556179No Hit
TTCTCACTACTGCACTTGACTAG296400.3842263419824446No Hit
CATTGCACTTGTCTCGGTCTGA295730.38335781415137765No Hit
CACCCGGCTGTGTGCACATGTGC287310.37244288230423805No Hit
CAACGGAATCCCAAAAGCAGCT262210.33990549639411877No Hit
CTTCTCACTACTGCACTTGACTAG259390.33624990164246393No Hit
TGAGGTAGTAGTTTGTACAGTT242450.3142904069286224No Hit
TAGCTTATCAGACTGATGTTGACA232630.30156064080761164No Hit
TGTAAACATCCTACACTCTCAGCT226910.29414574648865216No Hit
CCCCCCACTGCTAAATTTGACTGGCT221310.28688640939316734No Hit
AACATTCAACGCTGTCGGTGAGTT209190.2711751298222253No Hit
CTCCCACTGCTTCACTTGACTAGCCT207350.26878991906228034No Hit
CAAAGAATTCTCCTTTTGGGCT194220.2517693661937597No Hit
ACCCCCCACTGCTAAATTTGACTG161080.20880964631083732No Hit
TCAGTGCACTACAGAACTTTGT160980.20868001529127508No Hit
TCGTTTCCCGGCCAATGCACCA159690.20700777513892235No Hit
CAACACCAGTCGATGGGCTGT154900.2007984493018916No Hit
CCCCCCACTGCTAAATTTGACTGG153760.1993206556788822No Hit
TTCAAGTAATCCAGGATAGGCTT151570.1964817363504694No Hit
TTCAAGTAATCCAGGATAGGC140660.18233899211623028No Hit
TGATGTAGTAGGTTGTATAGTT134530.17439261061706568No Hit
TTATAAAGCAATGAGACTGATT129950.16845550992111563No Hit
CACTAGATTGTGAGCTCCTGGA129800.1682610633917723No Hit
TATTGCACTTGTCCCGGCCTGTAG129370.1677036500076547No Hit
CGCCTGTCTGAGCGTCGCT128150.1661221515689955No Hit
AACGGAATCCCAAAAGCAGCTG125680.16292026538580848No Hit
CACTAGATTGTGAGCTCCTGGAA125640.1628684129779836No Hit
TGAGGTAGTAGTTTGTGCTGTT123560.16017208777108924No Hit
TATTGCACTTGTCCCGGCCTGTT123470.16005541985348323No Hit
TAGCAGCACGTAAATATTGGC117250.15199237043671265No Hit
CAAAGAATTCTCCTTTTGGGCTT116800.15140903084868262No Hit
CCCATAAAGTAGAAAGCACTA110880.1437348744905987No Hit
AGCTCGGTCTGAGGCCCCTCAGT109630.14211448674607086No Hit
TTCACAGTGGCTAAGTTCCG108800.14103854928370435No Hit
TTCACAGTGGCTAAGTTCTGC108460.14059780381719278No Hit
TGATGTAGTAGATTGTATAGTT106960.13865333852375936No Hit
TGTCAGTTTGTCAAATACCCCA103950.13475144483493628No Hit
TTCACAGTGGCTAAGTTCTG103810.13456996140754918No Hit
TCAGTGCACTACAGAACTTTGTC100720.1305643629030763No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC94310.12225501454913748No Hit
ATCACATTGCCAGGGATTTCC89360.11583827908080717No Hit
TTTAAACATCCTTGACTGGAAGCT89040.11542345981820805No Hit
AGCAGCATTGTACAGGGCTATGT88190.1143215961519291No Hit
AGAATTACTCTGAGACCT86640.11231231534871457No Hit
TGTCAGTTTGTCAAATACCCC86150.11167712335285965No Hit
CAAAGTGCTGTTCGTGCAGGTAG86010.11149563992547253No Hit
TATTGCACTTGTCCCGGCCTGTTT85550.11089933723548628No Hit
TATTGCACTTGTCCCGGCCTGTAAT84680.1097715473652949No Hit
CAGTGCAATAGTATTGTCAAAGC83390.10809930721294216No Hit
TTCACAGTGGCTAAGTTCCGC83240.10790486068359881No Hit
TGTCAGTTTGTCAAATACCCCAA81680.10588261677842806No Hit
TATTGCACTTGTCCCGGCCTGTAAA81610.1057918750647345No Hit
TGTAAACATCCCCGACTGGAAGC80850.10480667931606155No Hit
CAAAGTGCTGTTCGTGCAGGT79970.10366592634391396No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)