FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13852396
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG186955813.496278910882998No Hit
TAGCTTATCAGACTGATGTTGAC5539163.998701740839635No Hit
AAGCTGCCAGTTGAAGAACTGT4072202.9397080476186215No Hit
TAGCAGCACGTAAATATTGGCG3558212.5686603241778534No Hit
TTCAAGTAATCCAGGATAGGCT3224572.3278066841288685No Hit
AACATTCAACGCTGTCGGTGAGT3006032.1700433628954876No Hit
TAGCTTATCAGACTGATGTTGACT2761411.9934529737671376No Hit
CCCCCCACTGCTAAATTTGACTG2373251.7132415215389454No Hit
AACATTCAACGCTGTCGGTGAG2340911.6898953798317635No Hit
TATTGCACTTGTCCCGGCCTGT2267741.637074192796683No Hit
TAGCTTATCAGACTGATGTTGA1942851.4025371495299441No Hit
TATTGCACTTGTCCCGGCCTGTAA1631791.17798393866303No Hit
TGTAAACATCCTTGACTGGAAGCT1533031.106689413152786No Hit
AGCAGCATTGTACAGGGCTATGA1501731.0840940440917224No Hit
TATTGCACTTGTCCCGGCCTGTAT1374570.9922976501682452No Hit
TATTGCACTTGTCCCGGCCTGTA1268350.9156177747156521No Hit
TCGTACGACTCTTAGCGG1238110.8937876162362092No Hit
CCCCCACTGCTAAATTTGACTG1185720.8559674441879945No Hit
TGAGGTAGTAGGTTGTATAGTT1064200.7682425480761595No Hit
TGTAAACATCCCCGACTGGAAGCT1025510.7403123618470047No Hit
CATAAAGTAGAAAGCACTACT973820.7029975175413697No Hit
TAGCTTATCAGACTGATGTTGACA951810.6871085695211139No Hit
ACTGGACTTGGAGTCAGAAGGC939760.678409713380992No Hit
CAACGGAATCCCAAAAGCAGCT844770.6098367387129273No Hit
CAACGGAATCCCAAAAGCAGCTGT805650.5815961368704735No Hit
CATTGCACTTGTCTCGGTCTGA799210.5769471216387404No Hit
TTCAAGTAATTCAGGATAGGTT727510.5251871228630772No Hit
CCCATAAAGTAGAAAGCACTAC638250.460750616716415No Hit
CGCCTGTCTGAGCGTCGCT540890.3904667466913305No Hit
CCCCCACTGCTAAATTTGACTGGCTT535520.3865901610089691No Hit
TTAATGCTAATCGTGATAGGGGTT522560.37723437880349364No Hit
TGAGGTAGTAGATTGTATAGTT504500.3641969230449375No Hit
CACCCGGCTGTGTGCACATGTGC499340.36047193568535No Hit
CAACACCAGTCGATGGGCTGT466270.336598809332335No Hit
TCGTTTCCCGGCCAATGCACCA423100.3054345255506701No Hit
TATTGCACTTGTCCCGGCCTGTT397060.28663633352670537No Hit
CACTAGATTGTGAGCTCCTGGA378190.27301414138030705No Hit
CCCATAAAGTAGAAAGCACT368380.2659323340164402No Hit
TGAGGTAGTAGTTTGTGCTGTT359760.2597095838149588No Hit
TAGCAGCACGTAAATATTGGC344030.24835414754241794No Hit
AACATTCAACGCTGTCGGTGAGTT324610.23433491216970698No Hit
AACGGAATCCCAAAAGCAGCTG322700.2329560893292395No Hit
CAAAGAATTCTCCTTTTGGGCT322230.23261679784493597No Hit
ACTTGACTTGGAGTCAGAAGGC314290.22688493745053204No Hit
CACTAGATTGTGAGCTCCTGGAA309590.2234920226074969No Hit
CAAAGTGCTGTTCGTGCAGGTAG299680.21633802556611867No Hit
TGATGTAGTAGGTTGTATAGTT297310.21462713020909885No Hit
CTCCCACTGCTTCACTTGACTAG289760.20917680955698925No Hit
ACCACAGGGTAGAACCACGGAC272060.19639923663747413No Hit
TTCACAGTGGCTAAGTTCCGC271270.1958289381851342No Hit
CTCCCACTGCTTCACTTGACTAGCCT271270.1958289381851342No Hit
TTATAAAGCAATGAGACTGATT268390.1937498754728063No Hit
AGCAGCATTGTACAGGGCTATGT264350.19083341250134633No Hit
TGTAAACATCCTACACTCTCAGCT254530.18374438616972832No Hit
CAAAGAATTCTCCTTTTGGGCTT252650.18238722023251427No Hit
AGCAGCATTGTACAGGGCTATG251920.18186023558668118No Hit
CTACGCCTGTCTGAGCGTCGCT251770.1817519510704141No Hit
CCCCCCACTGCTAAATTTGACTGGCT235600.17007888021682313No Hit
TATTGCACTTGTCCCGGCCTGTAG222610.16070144110809423No Hit
TTCAAGTAATCCAGGATAGGCTT220250.15899776471882554No Hit
TGTAAACATCCCCGACTGGAAGC203410.1468410230259083No Hit
TTCTCACTACTGCACTTGACTAG200650.14484858792659405No Hit
TTCACAGTGGCTAAGTTCTGC197420.14251686134297634No Hit
ACCGGGTGCTGTAGGCTT191400.13817104275679096No Hit
CTTCTCACTACTGCACTTGACTAG190980.13786784611124314No Hit
TTTAAACATCCTTGACTGGAAGCT183230.1322731461041108No Hit
TACGCCTGTCTGAGCGTCGCT181170.13078603874737627No Hit
TGAGGTAGTAGTTTGTACAGTT179150.12932780726164628No Hit
AGCTCGGTCTGAGGCCCCTCAGT178930.12916898997112125No Hit
TTCACCACCTTCTCCACCCAGC178920.1291617710033701No Hit
TAGCAGCACGTAAATATTGGCGT177910.1284326552605051No Hit
ATGCACCTGGGCAAGGATTCTGA171510.12381251589977647No Hit
TGTGCAAATCCATGCAAAACTG171190.12358150893174004No Hit
TGAGGTAGTAGGTTGTGTGGTT165470.11945225937808882No Hit
TATTGCACTTGTCCCGGCCTGTAAT163860.11829000557015551No Hit
ATCAGCATTGTACAGGGCTATGA154850.11178571562637973No Hit
ATCACATTGCCAGGGATTTCC153980.11115766543203066No Hit
CAACGGAATCCCAAAAGCAGCTGA153920.11111435162552384No Hit
TGTCAGTTTGTCAAATACCCCAA153080.11050795833442821No Hit
CACCCGTAGAACCGACCTTGCG151210.10915801136496531No Hit
TGTCAGTTTGTCAAATACCCCA149180.1076925609114842No Hit
ACTGGACTTGGAGTCAGAAGG148420.10714391936239767No Hit
AATGCACCCGGGCAAGGATTCT146190.10553408955389378No Hit
TAGCTTATCAGACTGATGTTGACC144190.10409029600366608No Hit
TGAGAACTGAATTCCATGGGTT143630.10368603380960233No Hit
TGATGTAGTAGATTGTATAGTT141810.10237218167889511No Hit
TGAGAACTGAATTCCATAGGCTGT141210.1019390436138268No Hit
TATTGCACTTGTCCCGGCCTGTAAA141160.10190294877507113No Hit
TCGTTTCCCGGCCAACGCACCA139550.10074069496713782No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)