FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7275311
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG78635810.808582615918413No Hit
AACATTCAACGCTGTCGGTGAGT3182654.374589622354288No Hit
AAGCTGCCAGTTGAAGAACTGT2723483.74345509078581No Hit
TAGCAGCACGTAAATATTGGCG2533973.4829713808797997No Hit
TTCAAGTAATCCAGGATAGGCT2095312.8800280840228No Hit
CCCCCCACTGCTAAATTTGACTG2087612.8694443440287296No Hit
AACATTCAACGCTGTCGGTGAG2007392.759181016454142No Hit
TATTGCACTTGTCCCGGCCTGTAA1357191.8654735172145904No Hit
TGAGGTAGTAGGTTGTATAGTT1104611.518299355175332No Hit
TATTGCACTTGTCCCGGCCTGTAT1092951.5022725488985968No Hit
TATTGCACTTGTCCCGGCCTGT1008461.3861400564182067No Hit
TAGCTTATCAGACTGATGTTGAC989921.3606566097311854No Hit
TAGCTTATCAGACTGATGTTGACT972081.3361353212254432No Hit
CCCCCACTGCTAAATTTGACTG943041.296219501819235No Hit
CGCCTGTCTGAGCGTCGCT942021.2948174998979425No Hit
TAGCTTATCAGACTGATGTTGA703360.9667765405492631No Hit
CATAAAGTAGAAAGCACTACT577330.7935468325683946No Hit
TGTAAACATCCTTGACTGGAAGCT550900.75721848866667No Hit
AGCAGCATTGTACAGGGCTATGA529170.727350349696391No Hit
TATTGCACTTGTCCCGGCCTGTA528480.7264019366319873No Hit
TGAGGTAGTAGATTGTATAGTT518360.7124918783540662No Hit
CAACGGAATCCCAAAAGCAGCTGT447090.6145304303829761No Hit
ACTGGACTTGGAGTCAGAAGGC417230.5734875113929837No Hit
TTCAAGTAATTCAGGATAGGTT405910.5579280390900128No Hit
TGTAAACATCCCCGACTGGAAGCT373940.5139849004393077No Hit
CATTGCACTTGTCTCGGTCTGA346660.4764882216031727No Hit
TCGTACGACTCTTAGCGG302720.4160921780525946No Hit
CAACACCAGTCGATGGGCTGT292270.4017285309177848No Hit
CCCATAAAGTAGAAAGCACT282520.3883270419642542No Hit
CCCATAAAGTAGAAAGCACTAC278540.3828564854478386No Hit
TTAATGCTAATCGTGATAGGGGTT276160.3795851476314896No Hit
CACCCGGCTGTGTGCACATGTGC271500.3731799231675457No Hit
TGAGGTAGTAGTTTGTACAGTT257500.35393675954196324No Hit
CAACGGAATCCCAAAAGCAGCT247080.3396143477577797No Hit
GGAATACCGGGTGCTGTAGGCTTT244670.33630177459080446No Hit
TGATGTAGTAGGTTGTATAGTT214070.29424171695203133No Hit
AACATTCAACGCTGTCGGTGAGTT213870.293966814614523No Hit
CTACGCCTGTCTGAGCGTCGCT211800.2911215754213119No Hit
TACGCCTGTCTGAGCGTCGCT207870.2857197444892734No Hit
GCCTGTCTGAGCGTCGCT206430.28374044765921347No Hit
ACGCCTGTCTGAGCGTCGCT206000.2831494076335706No Hit
ATCCCGGACGAGCCCCCA196780.270476409874437No Hit
TGTCAGTTTGTCAAATACCCCAA194510.2673562683437175No Hit
AACGGAATCCCAAAAGCAGCTG187030.25707492092090634No Hit
GCGTTCGCGCTTTCCCCTG174100.23930248480099342No Hit
TGAGGTAGTAGTTTGTGCTGTT172980.2377630317109468No Hit
GGAATACCGGGTGCTGTAGGCTT166490.22884245085880178No Hit
ACCGGGTGCTGTAGGCTT162520.22338563945926163No Hit
TTCTCACTACTGCACTTGACTAG152190.20918693372695682No Hit
TATTGCACTTGTCCCGGCCTGTT150260.20653412617000155No Hit
TATTGCACTTGTCCCGGCCTGTAG149100.20493969261245326No Hit
CACTAGATTGTGAGCTCCTGGA148440.20403251489867583No Hit
TGTAAACATCCTACACTCTCAGCT147770.20311159206802296No Hit
TGAGGTAGTAGGTTGTGTGGTT145930.20058249056294641No Hit
ACCACAGGGTAGAACCACGGAC140960.19375116747586463No Hit
CCCCCACTGCTAAATTTGACTGGCTT138400.1902324175557581No Hit
TCGTTTCCCGGCCAATGCACCA138000.18968261288074145No Hit
TAGCAGCACGTAAATATTGGC127040.17461796478528546No Hit
TGAGAACTGAATTCCATGGGTT119430.16415793084309385No Hit
TTCAAGTAATCCAGGATAGGCTT118910.16344318476557224No Hit
CTTCTCACTACTGCACTTGACTAG118900.16342943964869683No Hit
TTATAAAGCAATGAGACTGATT116070.15953957157295406No Hit
TAGCTTATCAGACTGATGTTGACA115300.15848119757354703No Hit
ATCCCACCGCTGCCACCA114950.15800011848290746No Hit
CTCCCACTGCTTCACTTGACTAG114110.15684552866537252No Hit
CACTAGATTGTGAGCTCCTGGAA111250.15291442523900353No Hit
TTCACAGTGGCTAAGTTCCGC109930.1511000698114486No Hit
ACCGGGTGCTGTAGGCTTT109460.15045404931830406No Hit
ACTGGACTTGGAGTCAGAAGGCT106390.14623429843755134No Hit
CAAAGAATTCTCCTTTTGGGCTT105300.144736080698131No Hit
TGTAAACATCCCCGACTGGAAGC103990.14293547038745147No Hit
CAAAGTGCTGTTCGTGCAGGTAG103970.14290798015370065No Hit
TTCACAGTGGCTAAGTTCTGC103910.14282550945244815No Hit
TGATGTAGTAGATTGTATAGTT103230.14189084150491985No Hit
AGCAGCATTGTACAGGGCTATGT102530.14092868332364075No Hit
ACTGGACTTGGAGTCAGAAGGCA100880.1386607390391971No Hit
CAAAGTGCTGTTCGTGCAGGT99510.1367776580272651No Hit
AGCAGCATTGTACAGGGCTATG98920.13596669613161555No Hit
TGAATACCGGGTGCTGTAGGCTTT97750.13435851745719185No Hit
TTCAAGTAATCCAGGATAGGC96820.13308022158777816No Hit
CAAAGAATTCTCCTTTTGGGCT96240.13228300480900404No Hit
ACTGGACTTGGAGTCAGAAGG94830.13034494332957036No Hit
TGTCAGTTTGTCAAATACCCCA94600.13002880564143582No Hit
ACCGGGTGCTGTAGGCTTA93470.12847560743451378No Hit
TATTGCACTTGTCCCGGCCTGTAAT92990.12781584182449382No Hit
AGCTCGGTCTGAGGCCCCTCAGT90670.12462697470939729No Hit
AGAGGTAGTAGGTTGCATAGTT88360.1214518527111762No Hit
TGAGGTAGTAGGTTGTATAGT88220.12125942107492037No Hit
AACCGGGCGGAAACACCA87480.12024228242613957No Hit
TTCACAGTGGCTAAGTTCTG87030.11962375216674585No Hit
ACTTGACTTGGAGTCAGAAGGC86770.11926637912798504No Hit
TGAGAACTGAATTCCATAGGCTGT85700.11779565162231553No Hit
CAACGGAATCCCAAAAGCAGCTGA85380.11735580788230222No Hit
TTCACAGTGGCTAAGTTCCG79140.1087788549520426No Hit
TATTGCACTTGTCCCGGCCTGTAAA78660.10811908934202263No Hit
TATTGCACTTGTCCCGGCCTGTTT78010.10722565674512058No Hit
TCGTTTCCCGGGCGGCGCACCA77170.10607106692758564No Hit
CCCCCCACTGCTAAATTTGACTGGCT76940.10575492923945105No Hit
AATGACACGATCACTCCCGTTG75310.10351447518875825No Hit
TCCTGTCTGAGCGTCGCT75000.10308837656562037No Hit
TAGCAGCACGTAAATATTGGCGT73700.10130151137181627No Hit
TGAGGTAGTAAGTTGTATTGTT73290.10073796157992422No Hit
TCGTTCGCGCTTTCCCCTG73200.10061425552804547No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)