FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10112305
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG110300910.907592284845048No Hit
CCCCCCACTGCTAAATTTGACTG4435294.386032660209517No Hit
TAGCAGCACGTAAATATTGGCG3970573.926473736699991No Hit
AACATTCAACGCTGTCGGTGAGT3811583.7692494441178344No Hit
AAGCTGCCAGTTGAAGAACTGT3246723.210662653074645No Hit
TTCAAGTAATCCAGGATAGGCT2722702.6924623021160854No Hit
AACATTCAACGCTGTCGGTGAG2655162.6256723862660394No Hit
TATTGCACTTGTCCCGGCCTGTAA2461022.433688461730535No Hit
TATTGCACTTGTCCCGGCCTGTAT2303582.277996955194686No Hit
TAGCTTATCAGACTGATGTTGACT1683771.6650704265743568No Hit
TAGCTTATCAGACTGATGTTGAC1630181.612075585141073No Hit
TGAGGTAGTAGGTTGTATAGTT1495101.478495753440981No Hit
GCCTGTCTGAGCGTCGCT1440641.4246405740333188No Hit
CCCCCACTGCTAAATTTGACTG1436441.4204872182949386No Hit
TATTGCACTTGTCCCGGCCTGT1229461.2158058919306725No Hit
TATTGCACTTGTCCCGGCCTGTA1021101.0097598915380817No Hit
TGTAAACATCCTTGACTGGAAGCT1012611.0013641795812132No Hit
TAGCTTATCAGACTGATGTTGA988660.9776801629302122No Hit
AGCAGCATTGTACAGGGCTATGA911720.9015946413799821No Hit
TGAGGTAGTAGATTGTATAGTT873670.863967216178705No Hit
ACTGGACTTGGAGTCAGAAGGC704440.6966166467486888No Hit
CATAAAGTAGAAAGCACTACT600810.5941375383752765No Hit
CAACGGAATCCCAAAAGCAGCTGT574180.5678032852054996No Hit
TGTAAACATCCCCGACTGGAAGCT562840.5565892247118733No Hit
TTAATGCTAATCGTGATAGGGGTT542730.5367025618788199No Hit
GGAATACCGGGTGCTGTAGGCTTT532790.5268729532979869No Hit
TTCAAGTAATTCAGGATAGGTT528050.522185594678958No Hit
CCCATAAAGTAGAAAGCACTAC479040.4737198887889556No Hit
CATTGCACTTGTCTCGGTCTGA444510.43957337125413054No Hit
TGAGGTAGTAGTTTGTACAGTT375580.37140889243352526No Hit
GGAATACCGGGTGCTGTAGGCTT372320.3681850972651636No Hit
TCGTACGACTCTTAGCGG353100.3491785502909574No Hit
TATTGCACTTGTCCCGGCCTGTAG338360.33460224943769No Hit
CGCCTGTCTGAGCGTCGCT304260.30088095641893714No Hit
TGTCAGTTTGTCAAATACCCCAA300840.29749893817482764No Hit
AACATTCAACGCTGTCGGTGAGTT297230.29392903002826754No Hit
CCCCCACTGCTAAATTTGACTGGCTT296890.2935928059923034No Hit
CACCCGGCTGTGTGCACATGTGC281990.2788582820632882No Hit
CCCATAAAGTAGAAAGCACT277590.27450714748022337No Hit
AACGGAATCCCAAAAGCAGCTG274510.27146135327207793No Hit
CACTAGATTGTGAGCTCCTGGA270260.2672585528225266No Hit
TGAGAACTGAATTCCATGGGTT266640.26367875573373234No Hit
CACTAGATTGTGAGCTCCTGGAA262150.25923862067055925No Hit
TGTAAACATCCTACACTCTCAGCT258820.2559456029065579No Hit
TATTGCACTTGTCCCGGCCTGTT247820.2450677664488957No Hit
TTCTCACTACTGCACTTGACTAG245840.24310975588651648No Hit
TGAGGTAGTAGTTTGTGCTGTT244720.24200219435628179No Hit
CAACACCAGTCGATGGGCTGT241770.23908495639718147No Hit
CTCCCACTGCTTCACTTGACTAG225550.2230450920932468No Hit
ACTGGACTTGGAGTCAGAAGGCA218500.21607338781810875No Hit
TAGCTTATCAGACTGATGTTGACA217510.21509438253691912No Hit
TAGCAGCACGTAAATATTGGC216800.2143922676382882No Hit
CTTCTCACTACTGCACTTGACTAG214040.2116629195816384No Hit
TCGTTTCCCGGGCGGCGCACCA211870.2095170191168087No Hit
ATCCCGGACGAGCCCCCA209130.20680744894462735No Hit
CAAAGAATTCTCCTTTTGGGCT204230.20196186724985055No Hit
TGAGAACTGAATTCCATAGGCTGT204080.20181353311633696No Hit
AACCGGGCGGAAACACCA192920.19077747358292693No Hit
ACCGGGTGCTGTAGGCTTA189760.18765256783690762No Hit
TCCTGTCTGAGCGTCGCT189090.18699000870721363No Hit
ACTGGACTTGGAGTCAGAAGGCT187220.18514077650941108No Hit
TATTGCACTTGTCCCGGCCTGTTT185640.1835783236364014No Hit
AGCTACATCTGGCTACTGGGTCTC184010.18196642605222052No Hit
TGTCAGTTTGTCAAATACCCCA179640.17764495829585836No Hit
CCCCCCACTGCTAAATTTGACTGGCT175800.1738476044779108No Hit
TATTGCACTTGTCCCGGCCTGTAAT175770.17381793765120812No Hit
AGCTCGGTCTGAGGCCCCTCAGT174610.17267082035203646No Hit
ACCGGGTGCTGTAGGCTT166820.16496733435156474No Hit
CTACGCCTGTCTGAGCGTCGCT165480.1636422160921768No Hit
AGCAGCATTGTACAGGGCTATGT163160.1613479814938335No Hit
CAACGGAATCCCAAAAGCAGCT158580.15681884595055232No Hit
TATTGCACTTGTCCCGGCCTGTAAA158080.15632439883884042No Hit
TGAGGTAGTAGGTTGTGTGGTT155790.1540598310671998No Hit
ACTGGACTTGGAGTCAGAAGG155660.1539312748181547No Hit
CAAAGTGCTGTTCGTGCAGGTAG153960.1522501546383342No Hit
TTCAAGTAATCCAGGATAGGCTT152790.15109314839692828No Hit
TTCACAGTGGCTAAGTTCCG147970.1463266782400254No Hit
CAAAGAATTCTCCTTTTGGGCTT143980.14238099028856427No Hit
TGTAAACATCCCCGACTGGAAGC142520.14093720472236548No Hit
ACCGGGTGCTGTAGGCTTT141070.13950330809840092No Hit
TACGCCTGTCTGAGCGTCGCT136000.13448961438564205No Hit
ACCACAGGGTAGAACCACGGAC135490.13398527833169588No Hit
AGCAGCATTGTACAGGGCTATG133100.13162182113771292No Hit
GAATACCGGGTGCTGTAGGCTTT131880.13041537018513583No Hit
CTCCCACTGCTTCACTTGACTAGCCT131770.1303065918205592No Hit
TTCAAGTAATCCAGGATAGGC127400.12598512406419704No Hit
TGAGGTAGTAGGTTGTATAGT118890.11756963422286018No Hit
AGAGGTAGTAGGTTGCATAGTT116700.11540395587356196No Hit
TAGCAGCACATCATGGTTTACA115280.1139997260763001No Hit
TAGCAGCACGTAAATATTGGCGT114510.11323827752426376No Hit
TTATAAAGCAATGAGACTGATT113680.11241749531882197No Hit
TTCACCACCTTCTCCACCCAGC112890.11163626888231713No Hit
AGCTACATTGTCTGCTGGGTTT112090.11084515350357807No Hit
TTCACAGTGGCTAAGTTCCGC111840.1105979299477221No Hit
TTCACAGTGGCTAAGTTCTGC111660.11041992898750581No Hit
CCCCCCACTGCTAAATTTGACTGG108770.10756202468181092No Hit
CAAAGTGCTGTTCGTGCAGGT108010.10681046507200881No Hit
CCCATAAAGTAGAAAGCACTA107510.10631601796029688No Hit
ACCCCCCACTGCTAAATTTGACTG104710.10354711413471014No Hit
TTCACAGTGGCTAAGTTCTG102330.10119354588296141No Hit
AACATTCAACGCTGTCGGTGAGA102260.10112432328732172No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)