FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10040729
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG127310212.679378160689328No Hit
CCCCCCACTGCTAAATTTGACTG5022755.002375823508433No Hit
AACATTCAACGCTGTCGGTGAGT4138524.121732595312552No Hit
TAGCAGCACGTAAATATTGGCG3895883.88007683505849No Hit
TTCAAGTAATCCAGGATAGGCT3194603.1816414923657437No Hit
AACATTCAACGCTGTCGGTGAG2912732.900914863851021No Hit
AAGCTGCCAGTTGAAGAACTGT2563392.55299191921224No Hit
CCCCCACTGCTAAATTTGACTG1896931.8892353334105523No Hit
TATTGCACTTGTCCCGGCCTGTAA1673781.6669905143341683No Hit
TATTGCACTTGTCCCGGCCTGTAT1501941.4958475624628451No Hit
TGAGGTAGTAGGTTGTATAGTT1427051.4212613446692965No Hit
TATTGCACTTGTCCCGGCCTGT1232511.2275104725961632No Hit
TAGCTTATCAGACTGATGTTGAC1167781.1630430419942617No Hit
TAGCTTATCAGACTGATGTTGACT1150361.1456937041125201No Hit
GCCTGTCTGAGCGTCGCT1010321.0062217593961553No Hit
TAGCTTATCAGACTGATGTTGA931250.9274724972658858No Hit
CATAAAGTAGAAAGCACTACT751510.7484615907868841No Hit
TGAGGTAGTAGATTGTATAGTT701520.6986743691618408No Hit
TATTGCACTTGTCCCGGCCTGTA700030.6971904131662153No Hit
TTCAAGTAATTCAGGATAGGTT686930.6841435517281663No Hit
TGTAAACATCCTTGACTGGAAGCT680320.6775603643918684No Hit
TCGTACGACTCTTAGCGG624550.6220165886361438No Hit
AGCAGCATTGTACAGGGCTATGA584500.5821290466060781No Hit
CAACGGAATCCCAAAAGCAGCTGT549670.5474403302788075No Hit
ACTGGACTTGGAGTCAGAAGGC505680.5036287703811148No Hit
CATTGCACTTGTCTCGGTCTGA500300.4982705936989237No Hit
CTCCCACTGCTTCACTTGACTAG470290.46838232562595805No Hit
CCCATAAAGTAGAAAGCACTAC425360.42363457872431376No Hit
TGTAAACATCCCCGACTGGAAGCT418570.41687212153619524No Hit
CACCCGGCTGTGTGCACATGTGC381290.37974334333692306No Hit
CCCATAAAGTAGAAAGCACT376010.37448476101685446No Hit
CGCCTGTCTGAGCGTCGCT370940.3694353268572431No Hit
CCCCCACTGCTAAATTTGACTGGCTT363300.3618263175910833No Hit
CAACACCAGTCGATGGGCTGT330430.32908965076141383No Hit
AACGGAATCCCAAAAGCAGCTG324140.3228251653839079No Hit
TGAGGTAGTAGTTTGTACAGTT309450.308194753588111No Hit
CACTAGATTGTGAGCTCCTGGA305010.3037727639098715No Hit
TTCTCACTACTGCACTTGACTAG274040.27292838996052976No Hit
AACATTCAACGCTGTCGGTGAGTT267410.26632528375180725No Hit
ACCGGGTGCTGTAGGCTT259130.25807887056806333No Hit
TGTAAACATCCTACACTCTCAGCT256580.2555392143339393No Hit
ATCCCGGACGAGCCCCCA250990.2499718894912909No Hit
CCCCCCACTGCTAAATTTGACTGGCT245540.24454399675561406No Hit
CACTAGATTGTGAGCTCCTGGAA239730.23875756431629616No Hit
ACCGGGTGCTGTAGGCTTA226530.22561110851612468No Hit
GGAATACCGGGTGCTGTAGGCTTT225660.22474463756565885No Hit
TGAGGTAGTAGTTTGTGCTGTT225410.22449565166035254No Hit
AGCTCGGTCTGAGGCCCCTCAGT219500.21860962485891214No Hit
TATTGCACTTGTCCCGGCCTGTAG218270.21738461420480526No Hit
TATTGCACTTGTCCCGGCCTGTT207430.20658858535072505No Hit
CTTCTCACTACTGCACTTGACTAG199790.19897957608456515No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC185280.18452843414058878No Hit
TGAGGTAGTAGGTTGTGTGGTT184520.1837715169884577No Hit
TAGCAGCACGTAAATATTGGC181450.1807139700712966No Hit
TTCAAGTAATCCAGGATAGGC179440.17871212339263415No Hit
GGAATACCGGGTGCTGTAGGCTT178680.17795520624050307No Hit
CAACGGAATCCCAAAAGCAGCT170520.16982830629130613No Hit
CTCCCACTGCTTCACTTGACTAGCCT164040.16337459162576742No Hit
ACCGGGTGCTGTAGGCTTT163310.16264755278227308No Hit
AACCGGGCGGAAACACCA154060.1534350742859408No Hit
CTACGCCTGTCTGAGCGTCGCT150940.15032773018771844No Hit
TTCACAGTGGCTAAGTTCCG150340.14973016401498337No Hit
TAGCTTATCAGACTGATGTTGACA149600.1489931657352768No Hit
TTCAAGTAATCCAGGATAGGCTT148840.14823624858314569No Hit
TTCACAGTGGCTAAGTTCTG147230.14663277935297328No Hit
CAAAGAATTCTCCTTTTGGGCT146190.14559699798689915No Hit
TTCACCACCTTCTCCACCCAGC144710.144123001427486No Hit
ACCACAGGGTAGAACCACGGAC141870.14129452154320668No Hit
TCGTTTCCCGGGCGGCGCACCA139570.1390038512143889No Hit
CAAAGTGCTGTTCGTGCAGGT137330.13677293750284467No Hit
TTCACAGTGGCTAAGTTCCGC132720.1321816374089969No Hit
AGAGGTAGTAGGTTGCATAGTT129880.12935315752471757No Hit
AATGACACGATCACTCCCGTTG127580.12706248719589983No Hit
ATCCCACCGCTGCCACCA127080.12656451538528726No Hit
CAAAGTGCTGTTCGTGCAGGTAG121970.12147524348082694No Hit
AGAATTACTCTGAGACCT121950.12145532460840244No Hit
AGCAGCATTGTACAGGGCTATGT121870.12137564911870442No Hit
GTCAGGATGGCCGAGCGGTCTAAGG120780.12029007057156905No Hit
TTCACAGTGGCTAAGTTCTGC120780.12029007057156905No Hit
TATTGCACTTGTCCCGGCCTGA120490.12000124692141377No Hit
TATTGCACTTGTCCCGGCCTGTTT120480.11999128748520153No Hit
CCCCCCACTGCTAAATTTGACTGG118560.1180790757324493No Hit
TGAGGTAGTAGGTTGTATAGT117500.11702337549395068No Hit
AACATTCAACGCTGTCGGTGA112140.1116851176841841No Hit
CCCCCACTGCTAAATTTGACTGGCT111050.1105995391370487No Hit
TTATAAAGCAATGAGACTGATT109970.1095239200261256No Hit
TACGCCTGTCTGAGCGTCGCT109380.10893631328960278No Hit
ACTGGACTTGGAGTCAGAAGGCT108570.10812959895641044No Hit
TATTGCACTTGTCCCGGCCTGTAAA106790.10635681931062974No Hit
ACTGGACTTGGAGTCAGAAGG106310.10587876637244169No Hit
AGCAGCATTGTACAGGGCTATG105460.10503221429440035No Hit
TCCTGTCTGAGCGTCGCT104170.10374744702301994No Hit
ACCCCCCACTGCTAAATTTGACTG103760.10333911013831765No Hit
CAAAGAATTCTCCTTTTGGGCTT102290.10187507301511672No Hit
TCCCACTGCTTCACTTGACTAG100990.10058034630752409No Hit
TATTGCACTTGTCCCGGCCTGTAAT100900.10049071138161383No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)