FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5313418
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG83811015.773462580960127No Hit
CAACGGAATCCCAAAAGCAGCTG54596210.275156217711462No Hit
CCCCCACTGCTAAATTTGACTG3255126.126226094013307No Hit
AACATTCAACGCTGTCGGTGAGT1668743.1406149487956716No Hit
TAGCAGCACGTAAATATTGGCG1349042.5389306845424167No Hit
AACATTCAACGCTGTCGGTGAG1298042.4429472704763677No Hit
TTCAAGTAATCCAGGATAGGCT1271712.3933934804301114No Hit
AAGCTGCCAGTTGAAGAACTGT1097842.066165319573954No Hit
TAGCTTATCAGACTGATGTTGAC1090252.051880729127654No Hit
TAGCTTATCAGACTGATGTTGACT961031.8086851062724598No Hit
TATTGCACTTGTCCCGGCCTGTAA794171.4946499597810674No Hit
TATTGCACTTGTCCCGGCCTGTAT643461.2110095610772575No Hit
TAGCTTATCAGACTGATGTTGA564781.0629316195337917No Hit
TGAGGTAGTAGGTTGTATAGTT401090.7548625009363088No Hit
TATTGCACTTGTCCCGGCCTGT352620.6636406170190262No Hit
TGTAAACATCCTTGACTGGAAGCT326490.6144632325181267No Hit
TATTGCACTTGTCCCGGCCTGTA321270.6046390477843075No Hit
AGCAGCATTGTACAGGGCTATGA278070.5233354499871834No Hit
TGTAAACATCCCCGACTGGAAGCT271940.5117986200219896No Hit
CAACGGAATCCCAAAAGCAGCT259010.4874640015146559No Hit
CATAAAGTAGAAAGCACTACT257460.48454685853813867No Hit
CAACGGAATCCCAAAAGCAGCTGT255290.4804628583710147No Hit
TTCAAGTAATTCAGGATAGGTT250490.4714291252824453No Hit
ACTGGACTTGGAGTCAGAAGGC224450.4224211232769566No Hit
CATTGCACTTGTCTCGGTCTGA221740.4173208281373684No Hit
TGAGGTAGTAGATTGTATAGTT219590.4132744685247801No Hit
CGCCCCACTGCTAAATTTGACTG206790.38918451362192846No Hit
TCGTACGACTCTTAGCGG167580.3153902064546776No Hit
CCCCCACTGCTAAATTTGACTGGCTT164840.31023345048328593No Hit
TAGCTTATCAGACTGATGTTGACA161860.30462500785746577No Hit
CCCGCCACTGCTAAATTTGACTG137310.2584212271648871No Hit
CACCCGGCTGTGTGCACATGTGC135670.25533470169295924No Hit
CAACACCAGTCGATGGGCTGT134060.2523046370528349No Hit
CAAAGAATTCTCCTTTTGGGCT131530.24754310690406817No Hit
GCCTGTCTGAGCGTCGCT131060.2466585538724791No Hit
CCCATAAAGTAGAAAGCACT127590.24012791766053415No Hit
CCCATAAAGTAGAAAGCACTAC127320.2396197701743021No Hit
TGATGTAGTAGGTTGTATAGTT125980.2370978530204098No Hit
AACATTCAACGCTGTCGGTGAGTT120080.2259938894323767No Hit
CTTCTCACTACTGCACTTGACTAG119620.22512815667805544No Hit
CCCTCCACTGCTAAATTTGACTG115020.21647082913484314No Hit
CACTAGATTGTGAGCTCCTGGA114960.216357907471236No Hit
TTCTCACTACTGCACTTGACTAG113910.2143817783581115No Hit
ACCCCCCACTGCTAAATTTGACTG113600.21379834976280807No Hit
TAGCAGCACGTAAATATTGGC111010.20892389795043415No Hit
CACTAGATTGTGAGCTCCTGGAA110300.20758765826441664No Hit
CAAAGAATTCTCCTTTTGGGCTT105970.19943847820743632No Hit
ACTTGACTTGGAGTCAGAAGGC101570.1911575562095811No Hit
TTTAAACATCCTTGACTGGAAGCT99240.1867724316061714No Hit
TGTAAACATCCTACACTCTCAGCT98300.18500332554299323No Hit
TATTGCACTTGTCCCGGCCTGTAG97350.18321539920254723No Hit
CGACGGAATCCCAAAAGCAGCTG95980.18063702121685138No Hit
CCCCCCACTGCTAAATTTGACTGG93630.17621425605890598No Hit
AACGGAATCCCAAAAGCAGCTG91890.17293952781429958No Hit
TTCAAGTAATCCAGGATAGGCTT87610.16488444914365857No Hit
GCCCCCACTGCTAAATTTGACTG86320.16245663337610555No Hit
CCCCCCACTGCTAAATTTGACTGGCT80400.15131502923353668No Hit
TTTAAACATCCCCGACTGGAAGCT80140.15082570202457252No Hit
TGAGGTAGTAGTTTGTACAGTT79140.1489436742977872No Hit
CTCCCCACTGCTAAATTTGACTG75820.1426953422448601No Hit
CGCCCACTGCTAAATTTGACTG75780.14262006113578868No Hit
TATTGCACTTGTCCCGGCCTGTAAT75710.1424883191949137No Hit
CAAGGGAATCCCAAAAGCAGCTG73870.1390253881776288No Hit
ATCAGCATTGTACAGGGCTATGA73410.13815965542330755No Hit
TCAGTGCACTACAGAACTTTGT72300.1360706046465759No Hit
CTCCCACTGCTTCACTTGACTAGCCT70680.13302171972918375No Hit
TCAGTGCACTACAGAACTTTGTC69960.13166665976589834No Hit
TTAGGTAGTAGGTTGTATAGTT69850.13145963671595196No Hit
TTATGTAGTAGGTTGTATAGTT69310.1304433417434879No Hit
TGATGTAGTAGATTGTATAGTT68880.12963406982097023No Hit
CAATGGAATCCCAAAAGCAGCTG68450.12882479789845255No Hit
TATTGCACTTGTCCCGGCCTGTAAA68150.12826018958041696No Hit
TCCTGTCTGAGCGTCGCT67690.12739445682609576No Hit
TATTGCACTTGTCCCGGCCTGTT67350.12675456739898874No Hit
CTCCCACTGCTTCACTTGACTAG67160.12639698213089953No Hit
ACTGGACTTGGAGTCAGAAGGCA66830.12577591298106042No Hit
ACTGGACTTGGAGTCAGAAGGCT66730.12558771020838186No Hit
TCGTTTCCCGGCCAATGCACCA64920.12218124002290051No Hit
ATGCACCTGGGCAAGGATTCTGA64910.12216241974563266No Hit
TGAGGTAGTAGTTTGTGCTGTT64660.12169191281393633No Hit
TTATAAAGCAATGAGACTGATT62680.11796549791490148No Hit
TGTAAACATCCCCGACTGGAAGC62080.1168362812788303No Hit
CAACGGAATCCCAAAAGCAGCTGA61500.11574470519729485No Hit
ATACATGATGATCTCAATCCAAC59970.11286520277531337No Hit
TGCAAGTAATCCAGGATAGGCT57940.10904468648993924No Hit
ATCACATTGCCAGGGATTTCC54480.10253287055526215No Hit
TTCACAGTGGCTAAGTTCCGC54400.10238230833711935No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)