FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8482588
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6783387.996828326449427No Hit
CAACGGAATCCCAAAAGCAGCTG5324476.276940480900405No Hit
AACATTCAACGCTGTCGGTGAGT3508854.136532388464464No Hit
AAGCTGCCAGTTGAAGAACTGT3328313.923696400202391No Hit
CCCCCACTGCTAAATTTGACTG3185413.7552336621795144No Hit
TTCAAGTAATCCAGGATAGGCT2891423.4086531138845833No Hit
TAGCAGCACGTAAATATTGGCG2762603.2567890837088873No Hit
AACATTCAACGCTGTCGGTGAG1881962.218615356539773No Hit
TAGCTTATCAGACTGATGTTGAC1546241.8228399163085607No Hit
TAGCTTATCAGACTGATGTTGACT1308231.5422533783321788No Hit
TAGCTTATCAGACTGATGTTGA1018061.2001761726491962No Hit
CATAAAGTAGAAAGCACTACT976301.150945914147899No Hit
TATTGCACTTGTCCCGGCCTGTAA924631.090032900336548No Hit
TGAGGTAGTAGGTTGTATAGTT894491.0545012913511773No Hit
TGTAAACATCCTTGACTGGAAGCT829900.9783570768732373No Hit
TATTGCACTTGTCCCGGCCTGT795630.93795667077076No Hit
TATTGCACTTGTCCCGGCCTGTAT756710.8920744470909113No Hit
CGCCTGTCTGAGCGTCGCT694590.8188420797992311No Hit
AGCAGCATTGTACAGGGCTATGA611030.7203344073766166No Hit
TGAGGTAGTAGATTGTATAGTT502540.5924371194262883No Hit
TGTAAACATCCCCGACTGGAAGCT482140.5683878552158846No Hit
TATTGCACTTGTCCCGGCCTGTA478020.5635308469537834No Hit
TTCAAGTAATTCAGGATAGGTT467270.5508578278233012No Hit
GCCTGTCTGAGCGTCGCT442090.5211734909204596No Hit
ACTGGACTTGGAGTCAGAAGGC415870.49026311309708787No Hit
CCCATAAAGTAGAAAGCACTAC333250.3928635930449528No Hit
TGATGTAGTAGGTTGTATAGTT322170.37980154169930214No Hit
CCCATAAAGTAGAAAGCACT317150.37388353648674205No Hit
CATTGCACTTGTCTCGGTCTGA316140.37269286213122693No Hit
CAACACCAGTCGATGGGCTGT300020.353689227863006No Hit
TGTCAGTTTGTCAAATACCCCAA298520.35192089961224093No Hit
CAACGGAATCCCAAAAGCAGCTGT296640.3497045948712822No Hit
AACATTCAACGCTGTCGGTGAGTT287110.33846981605142207No Hit
TGAGGTAGTAGTTTGTACAGTT250760.29561732810788405No Hit
TTCTCACTACTGCACTTGACTAG246970.2911493520609512No Hit
TCGTACGACTCTTAGCGG246940.2911139854959359No Hit
TGTAAACATCCTACACTCTCAGCT236000.2782169781203567No Hit
TTCAAGTAATCCAGGATAGGCTT188350.22204308402105585No Hit
TGATGTAGTAGATTGTATAGTT182790.21548848063822035No Hit
CTACGCCTGTCTGAGCGTCGCT179790.21195182413669034No Hit
TTATAAAGCAATGAGACTGATT178060.20991235222080806No Hit
TACGCCTGTCTGAGCGTCGCT175000.20630496258924755No Hit
TTAATGCTAATCGTGATAGGGGTT170540.201047133256973No Hit
TCCTGTCTGAGCGTCGCT168800.19899587248608563No Hit
ACTTGACTTGGAGTCAGAAGGC164570.19400918681892837No Hit
CTTCTCACTACTGCACTTGACTAG160890.1896708881770516No Hit
TGTCAGTTTGTCAAATACCCCA159750.18832695870647023No Hit
TAGCAGCACGTAAATATTGGC156960.18503786816004736No Hit
TGCAAGTAATCCAGGATAGGCT156740.18477851334993517No Hit
TAGCTTATCAGACTGATGTTGACA156490.1844837919748077No Hit
ACGCCTGTCTGAGCGTCGCT151810.17896660783242097No Hit
TGAGGTAGTAGTTTGTGCTGTT148030.1745104206404932No Hit
ACCACAGGGTAGAACCACGGAC146320.17249452643462113No Hit
TGTAAACATCCTACACTCAGCT143830.16955910153835127No Hit
TGTAAACATCCCCGACTGGAAGC143350.1689932364981065No Hit
AACCGGGCGGAAACACCA140480.16560983511164282No Hit
TTCAAGTAATCCAGGATAGGC127940.1508266109352476No Hit
AGCAGCATTGTACAGGGCTATGT126280.14886966100440102No Hit
CAAAGAATTCTCCTTTTGGGCTT124280.14651189000338102No Hit
GGAATACCGGGTGCTGTAGGCTTT123980.14615822435322803No Hit
CACCCGGCTGTGTGCACATGTGC123300.14535658221288125No Hit
TTCACAGTGGCTAAGTTCCGC123060.14507364969275888No Hit
TATTGCACTTGTCCCGGCCTGTT122970.14496754999771297No Hit
AACATTCAACGCTGTCGGTGA120190.1416902483062952No Hit
CAACGGAATCCCAAAAGCAGCT113330.1336030937727967No Hit
TAGCAGCACGTAAATATTGGCGT112310.13240063056227652No Hit
CAAAGAATTCTCCTTTTGGGCT112100.1321530646071694No Hit
TTCACAGTGGCTAAGTTCCG111250.13115101193173592No Hit
AACGGAATCCCAAAAGCAGCTG110680.13047904719644524No Hit
TTCACAGTGGCTAAGTTCTGC110360.13010180383628203No Hit
CCCCCCACTGCTAAATTTGACTGG110000.12967740505609845No Hit
TATTGCACTTGTCCCGGCCTGTAG108680.12812127619542527No Hit
AGCAGCATTGTACAGGGCTATG108100.12743752260512947No Hit
CTCCCACTGCTTCACTTGACTAG106610.1256809832093696No Hit
CCCCCACTGCTAAATTTGACTGGCTT105920.12486755221401771No Hit
GCGTTCGCGCTTTCCCCTG105400.12425453175375252No Hit
CGCCCCACTGCTAAATTTGACTG105120.1239244438136097No Hit
ATCCCACCGCTGCCACCA104750.12348825617842102No Hit
CAAAGTGCTGTTCGTGCAGGT103030.12146057311754385No Hit
ACTGGACTTGGAGTCAGAAGGCA101020.11909101326151877No Hit
CTCCCCACTGCTAAATTTGACTG100010.11790033890600368No Hit
ACTGGACTTGGAGTCAGAAGGCT99250.1170043859256161No Hit
ACTGGACTTGGAGTCAGAAGG97020.11437547125947883No Hit
CCCGCCACTGCTAAATTTGACTG96600.11388033934926463No Hit
CAAAGTGCTGTTCGTGCAGGTAG96270.11349130713409634No Hit
GGAATACCGGGTGCTGTAGGCTT93410.11011969460263778No Hit
TTTAAACATCCTTGACTGGAAGCT93310.11000180605258679No Hit
TGATGTAGTAGTTTGTACAGTT91690.1080920115417606No Hit
TGAGGTAGTAGGTTGTGTGGTT91640.1080330672667351No Hit
ATCCCGGACGAGCCCCCA89460.10546309687562334No Hit
CACTAGATTGTGAGCTCCTGGA87810.10351793579978187No Hit
TTCACAGTGGCTAAGTTCTG86340.10178497411403217No Hit
AACATTCAACGCTGTCGGTGAGA85830.10118374250877209No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)