FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12852175
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG141677811.023643857946224No Hit
CAACGGAATCCCAAAAGCAGCTG9730697.571239887412053No Hit
CCCCCACTGCTAAATTTGACTG5795154.509081147743475No Hit
AAGCTGCCAGTTGAAGAACTGT5164314.0182381581327675No Hit
TAGCAGCACGTAAATATTGGCG4985613.879195544722975No Hit
TTCAAGTAATCCAGGATAGGCT4281933.331677323099008No Hit
AACATTCAACGCTGTCGGTGAGT4264883.3184110860613085No Hit
TAGCTTATCAGACTGATGTTGAC2602612.0250346731195306No Hit
AACATTCAACGCTGTCGGTGAG2250231.7508553999614853No Hit
TAGCTTATCAGACTGATGTTGACT1921541.4951088045408658No Hit
TAGCTTATCAGACTGATGTTGA1582511.2313168782715767No Hit
GCCTGTCTGAGCGTCGCT1423021.1072211512837322No Hit
TATTGCACTTGTCCCGGCCTGTAA1272250.9899102681063711No Hit
TGTAAACATCCTTGACTGGAAGCT1217300.9471548590024645No Hit
CATAAAGTAGAAAGCACTACT1151970.8963229959131432No Hit
TATTGCACTTGTCCCGGCCTGTAT1044180.8124539231686465No Hit
TGAGGTAGTAGGTTGTATAGTT1026680.7988375508425617No Hit
TATTGCACTTGTCCCGGCCTGT983980.7656136023669146No Hit
AGCAGCATTGTACAGGGCTATGA925390.7200259878191824No Hit
TCCTGTCTGAGCGTCGCT825730.6424826926181755No Hit
TTCAAGTAATTCAGGATAGGTT662450.5154380484237103No Hit
TATTGCACTTGTCCCGGCCTGTA646140.5027475894157992No Hit
TGTAAACATCCCCGACTGGAAGCT619490.4820117995592186No Hit
ACTGGACTTGGAGTCAGAAGGC611700.47595056867806423No Hit
CAACGGAATCCCAAAAGCAGCTGT582120.45293500905488765No Hit
TGAGGTAGTAGATTGTATAGTT557740.4339654572086048No Hit
CGCCTGTCTGAGCGTCGCT511580.398049357404486No Hit
CCCATAAAGTAGAAAGCACTAC479880.37338427153380654No Hit
TTCTCACTACTGCACTTGACTAG465770.36240558504688897No Hit
CATTGCACTTGTCTCGGTCTGA457550.35600978044572223No Hit
TTAATGCTAATCGTGATAGGGGTT451610.3513879946390397No Hit
CCCATAAAGTAGAAAGCACT436650.33974794149628373No Hit
TGTAAACATCCTACACTCTCAGCT418280.32545464094598775No Hit
CAACGGAATCCCAAAAGCAGCT390030.3034739256195936No Hit
CTTCTCACTACTGCACTTGACTAG381840.29710146337098586No Hit
AACATTCAACGCTGTCGGTGAGTT377380.2936312336238808No Hit
TCGTACGACTCTTAGCGG374660.29151486032519786No Hit
TGATGTAGTAGGTTGTATAGTT372320.2896941568255957No Hit
CAACACCAGTCGATGGGCTGT289630.22535485238879802No Hit
AACCGGGCGGAAACACCA284940.22170566460540725No Hit
TAGCTTATCAGACTGATGTTGACA276720.21530986000424052No Hit
TAGCAGCACGTAAATATTGGC260060.20234707354980772No Hit
CCCCCCACTGCTAAATTTGACTGG259510.20191913041955933No Hit
CAAAGAATTCTCCTTTTGGGCT258930.20146784493675196No Hit
CCCCCACTGCTAAATTTGACTGGCTT253470.19721953677101348No Hit
TTCAAGTAATCCAGGATAGGCTT251120.1953910524872249No Hit
ACTTGACTTGGAGTCAGAAGGC247810.1928156129215483No Hit
CTACGCCTGTCTGAGCGTCGCT247650.19269112037456695No Hit
TGAGGTAGTAGTTTGTACAGTT244360.19013124237726298No Hit
CTCCCCACTGCTAAATTTGACTG235300.18308185190444418No Hit
CAAAGAATTCTCCTTTTGGGCTT228850.1780632461042586No Hit
CTCCCACTGCTTCACTTGACTAG227340.17688834769212214No Hit
TGCAAGTAATCCAGGATAGGCT223430.17384606107526546No Hit
CGCCCCACTGCTAAATTTGACTG208750.16242386988972682No Hit
TGATGTAGTAGATTGTATAGTT204850.1593893640570565No Hit
TGTAAACATCCCCGACTGGAAGC190540.14825506188641224No Hit
AACGGAATCCCAAAAGCAGCTG188490.1466600011282137No Hit
TGAGGTAGTAGTTTGTGCTGTT188170.14641101603425102No Hit
CCCCCCACTGCTAAATTTGACTGGCT188080.146340988976574No Hit
TTATAAAGCAATGAGACTGATT187650.14600641525656163No Hit
ACCACAGGGTAGAACCACGGAC187550.1459286074146983No Hit
TGTAAACATCCTACACTCAGCT185120.1440378768574191No Hit
TTCACAGTGGCTAAGTTCCG184010.14317420981273596No Hit
TACGCCTGTCTGAGCGTCGCT183830.14303415569738195No Hit
TTCAAGTAATCCAGGATAGGC183060.14243503531503424No Hit
AGCTCGGTCTGAGGCCCCTCAGT175700.13670837815389225No Hit
CACCCGGCTGTGTGCACATGTGC171870.13372833781052623No Hit
CCCGCCACTGCTAAATTTGACTG169430.13182982646906066No Hit
CACTAGATTGTGAGCTCCTGGA167180.1300791500271355No Hit
TAGCAGCACGTAAATATTGGCGT164920.12832069280102396No Hit
TATTGCACTTGTCCCGGCCTGTT164680.12813395398055194No Hit
ACCCCCCACTGCTAAATTTGACTG164410.1279238728075209No Hit
AGCAGCATTGTACAGGGCTATGT163960.12757373751913587No Hit
CAAAGTGCTGTTCGTGCAGGT157560.12259403563988197No Hit
TGTCAGTTTGTCAAATACCCCA156660.12189376506311189No Hit
TATTGCACTTGTCCCGGCCTGTAG151570.11793334591226777No Hit
TTTAAACATCCTTGACTGGAAGCT151410.11780885336528642No Hit
TTCACAGTGGCTAAGTTCTGC151160.11761433376062805No Hit
GACCCGCGGGCGCTCTCCAGTCCT149810.11656392789547296No Hit
AGCAGCATTGTACAGGGCTATG149700.11647833926942328No Hit
ACTGGACTTGGAGTCAGAAGGCA149360.1162137926070879No Hit
ACTGGACTTGGAGTCAGAAGG148580.11560689144055383No Hit
ACGCCTGTCTGAGCGTCGCT147170.11450980087028072No Hit
AACATTCAACGCTGTCGGTGA142590.11094620171293963No Hit
GCGTTCGCGCTTTCCCCTG137700.1071413982458222No Hit
CACTAGATTGTGAGCTCCTGGAA134170.10439478142804622No Hit
CAAAGTGCTGTTCGTGCAGGTAG132860.10337549869963644No Hit
TGTCAGTTTGTCAAATACCCCAA128900.10029430816184809No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)