FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12008785
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT10684938.897594552654578No Hit
CAACGGAATCCCAAAAGCAGCTG9930048.268979751073902No Hit
CCCCCCACTGCTAAATTTGACTG9462877.879956215387319No Hit
AAGCTGCCAGTTGAAGAACTGT3868473.221366691134865No Hit
TAGCAGCACGTAAATATTGGCG3662973.0502419686920867No Hit
TCCTGTCTGAGCGTCGCT3567622.970841762926058No Hit
TTCAAGTAATCCAGGATAGGCT3466412.8865617962183516No Hit
CCCCCACTGCTAAATTTGACTG3441232.8655938131959227No Hit
AACATTCAACGCTGTCGGTGAGT2963052.467401989460216No Hit
TAGCTTATCAGACTGATGTTGAC1928291.6057328031103897No Hit
AACATTCAACGCTGTCGGTGAG1634561.3611368677180915No Hit
TAGCTTATCAGACTGATGTTGACT1212651.009802407154429No Hit
TGTAAACATCCTTGACTGGAAGCT1116470.9297110407089476No Hit
TATTGCACTTGTCCCGGCCTGTAA1090900.908418295439547No Hit
TAGCTTATCAGACTGATGTTGA1010530.841492290852072No Hit
AGCAGCATTGTACAGGGCTATGA937880.7809949133072164No Hit
TGAGGTAGTAGGTTGTATAGTT932880.7768312947562972No Hit
CATAAAGTAGAAAGCACTACT879690.732538720611619No Hit
TATTGCACTTGTCCCGGCCTGTAT879330.7322389400759527No Hit
TATTGCACTTGTCCCGGCCTGT867580.7224544364812927No Hit
CGCCTGTCTGAGCGTCGCT664280.5531617062009188No Hit
TGTAAACATCCCCGACTGGAAGCT636870.5303367493047798No Hit
CCCATAAAGTAGAAAGCACTAC607850.5061711072352448No Hit
CAACGGAATCCCAAAAGCAGCTGT596740.4969195468151024No Hit
TATTGCACTTGTCCCGGCCTGTA564230.46984769899702594No Hit
TTCAAGTAATTCAGGATAGGTT536440.4467063070910171No Hit
ACTGGACTTGGAGTCAGAAGGC535580.445990164700259No Hit
GGAATACCGGGTGCTGTAGGCTTT515920.4296188165580448No Hit
TGTCAGTTTGTCAAATACCCCAA510840.4253885801103109No Hit
TGAGGTAGTAGATTGTATAGTT495360.4124980170766651No Hit
AACCGGGCGGAAACACCA465200.3873830699775206No Hit
TTCTCACTACTGCACTTGACTAG388430.32345487074670753No Hit
TGTAAACATCCTACACTCTCAGCT381330.3175425324044023No Hit
GGAATACCGGGTGCTGTAGGCTT355630.29614153305267765No Hit
CATTGCACTTGTCTCGGTCTGA353780.29460099418883756No Hit
CCCCCACTGCTAAATTTGACTGGCTT349390.2909453371011306No Hit
TGTCAGTTTGTCAAATACCCCA304900.25389745923505164No Hit
CTTCTCACTACTGCACTTGACTAG303180.25246517445353545No Hit
CCCTGTCTGAGCGTCGCT297400.24765203140867292No Hit
AACATTCAACGCTGTCGGTGAGTT296830.24717737889386812No Hit
CCCATAAAGTAGAAAGCACT284400.23682662317628303No Hit
CTACGCCTGTCTGAGCGTCGCT277330.2309392665452833No Hit
TAGCTTATCAGACTGATGTTGACA260350.21679961794636177No Hit
TAGCAGCACGTAAATATTGGC247490.20609079103339764No Hit
CAAAGAATTCTCCTTTTGGGCT237070.1974138099732821No Hit
TGATGTAGTAGGTTGTATAGTT230590.1920177603312908No Hit
TGAGGTAGTAGTTTGTACAGTT226200.18836210324358377No Hit
CCCCCCACTGCTAAATTTGACTGG208610.17371449318145007No Hit
CAACGGAATCCCAAAAGCAGCT206090.1716160294317868No Hit
TGAATACCGGGTGCTGTAGGCTTT204550.17033363491810372No Hit
CCCCCCACTGCTAAATTTGACTGGCT204520.1703086532067982No Hit
TAGCAGCACGTAAATATTGGCGT202850.16891800461079118No Hit
TGTAAACATCCCCGACTGGAAGC202020.1682268439313386No Hit
CTCCCACTGCTTCACTTGACTAG201970.1681852077458294No Hit
TACGCCTGTCTGAGCGTCGCT196150.16333875575255946No Hit
TGTAAACATCCTACACTCAGCT193230.16090720251882268No Hit
TGAGGTAGTAGTTTGTGCTGTT191460.1594332815517973No Hit
AACGGAATCCCAAAAGCAGCTG187270.15594416920612703No Hit
GACCCGCGGGCGCTCTCCAGTCCT185670.15461181126983287No Hit
CAAAGAATTCTCCTTTTGGGCTT184700.15380406927095455No Hit
CAACACCAGTCGATGGGCTGT183120.1524883658088641No Hit
TTCAAGTAATCCAGGATAGGCTT182670.15211364013928136No Hit
ACCCCCCACTGCTAAATTTGACTG177900.14814154804170448No Hit
AGCAGCATTGTACAGGGCTATGT170870.14228750035911208No Hit
CACCCGGCTGTGTGCACATGTGC167510.13948954869289443No Hit
GTCAGGATGGCCGAGCGGTCTAAGG159430.13276114111460902No Hit
TTCACAGTGGCTAAGTTCCGC156920.13067100460204759No Hit
AGCTCGGTCTGAGGCCCCTCAGT155310.1293303194286516No Hit
GCCTGTCTGAGCGTCGCA155130.1291804291608185No Hit
TTCAAGTAATCCAGGATAGGC154710.1288306852025413No Hit
GGGCTACGCCTGTCTGAGCGTCGCT154200.12840599611034753No Hit
ACGCCTGTCTGAGCGTCGCT153610.1279146891213391No Hit
TTCACAGTGGCTAAGTTCCG152190.12673222145287805No Hit
TATTGCACTTGTCCCGGCCTGTT151890.12648240433982288No Hit
TTCACAGTGGCTAAGTTCTGC149120.12417575966261366No Hit
ATCACATTGCCAGGGATTTCC148340.12352623516867027No Hit
ACTTGACTTGGAGTCAGAAGGC145040.12077824692506361No Hit
ACCTGTCTGAGCGTCGCT144000.11991221426647242No Hit
AGCAGCATTGTACAGGGCTATG142310.11850491119626175No Hit
ACCACAGGGTAGAACCACGGAC141890.11815516723798453No Hit
ATCACATTGCCAGGGATTTCCA140430.11693939062111611No Hit
TGAATACCGGGTGCTGTAGGCTT139910.11650637429182054No Hit
ACTGGACTTGGAGTCAGAAGGCA136060.11330038800761277No Hit
CAAAGTGCTGTTCGTGCAGGTAG135180.11256759114265098No Hit
TAGCAGCACATCATGGTTTACA133150.11087716201097779No Hit
CTCCCACTGCTTCACTTGACTAGCCT132610.11042749120747852No Hit
ATCCCACCGCTGCCACCA127390.10608067344031889No Hit
TGATGTAGTAGATTGTATAGTT125680.10465671589590453No Hit
TGAGGTAGTAGGTTGTGTGGTT120750.10055138800469823No Hit
TTATAAAGCAATGAGACTGATT120270.10015168062380997No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)