FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8142564
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTGCCAGTTGAAGAACTGT6999058.59563400422766No Hit
CAACGGAATCCCAAAAGCAGCTG6730968.266388818067625No Hit
CCCCCCACTGCTAAATTTGACTG3177803.9027019007772No Hit
TTCAAGTAATCCAGGATAGGCT2275212.794218135712535No Hit
TGTAAACATCCTTGACTGGAAGCT1655932.0336714577865154No Hit
TAGCTTATCAGACTGATGTTGACT1463771.797676997073649No Hit
ACTGGACTTGGAGTCAGAAGGC1280141.57215835208664No Hit
TAGCTTATCAGACTGATGTTGAC1239841.5226653422681111No Hit
CCCCCACTGCTAAATTTGACTG1169681.4365008368371437No Hit
TGTAAACATCCCCGACTGGAAGCT1108701.3616104214839453No Hit
TATTGCACTTGTCCCGGCCTGTAA1016261.248083527498218No Hit
CACCCGGCTGTGTGCACATGTGC987561.2128366445753451No Hit
TAGCTTATCAGACTGATGTTGA949191.1657138955248003No Hit
AACATTCAACGCTGTCGGTGAGT907081.113997998664794No Hit
ACCGGGTGCTGTAGGCTT882161.083393388126885No Hit
TATTGCACTTGTCCCGGCCTGTAT856571.0519659409493127No Hit
CAACACCAGTCGATGGGCTGT797930.9799493132629967No Hit
AACATTCAACGCTGTCGGTGAG752130.9237016743129067No Hit
CGCCTGTCTGAGCGTCGCT636920.7822106157225169No Hit
TCGTACGACTCTTAGCGG590810.7255822613122844No Hit
ACCGGGTGCTGTAGGCTTT585690.719294315647995No Hit
TATTGCACTTGTCCCGGCCTGT579390.7115571950063886No Hit
TAGCAGCACGTAAATATTGGCG568470.6981461858942711No Hit
AGCAGCATTGTACAGGGCTATGA534620.6565745138754819No Hit
CAACGGAATCCCAAAAGCAGCTGT527250.6475233108391901No Hit
TATTGCACTTGTCCCGGCCTGTA436090.5355684032695353No Hit
CATAAAGTAGAAAGCACTACT403740.4958389028320809No Hit
ACCGGGTGCTGTAGGCTTA388000.4765083823719408No Hit
TGAGGTAGTAGGTTGTATAGTT374340.4597323398379184No Hit
CACTAGATTGTGAGCTCCTGGA357700.4392965164289774No Hit
TGTAAACATCCCCGACTGGAAGC334270.4105217963285275No Hit
TGAGGTAGTAGTTTGTGCTGTT317160.38950875915743494No Hit
CCCATAAAGTAGAAAGCACTAC313440.3849401736357246No Hit
ACCACAGGGTAGAACCACGGAC313170.38460858275108434No Hit
ACTGGACTTGGAGTCAGAAGGCT294140.3612375659558832No Hit
ACTGGACTTGGAGTCAGAAGG280570.3445720537167408No Hit
CCCATAAAGTAGAAAGCACT273510.33590156614059163No Hit
CACTAGATTGTGAGCTCCTGGAA260730.3202062642676189No Hit
TGTAAACATCCCCGACTGGA233370.2866050546240717No Hit
TGTAAACATCCTACACTCTCAGCT229750.28215928054111705No Hit
TGAGGTAGTAGGTTGTGTGGTT225450.2768783886746239No Hit
CAAAGAATTCTCCTTTTGGGCT218290.268085089659719No Hit
TGAGAACTGAATTCCATGGGTT209540.2573390887685992No Hit
TCCACCACGTTCCCGTGG190550.23401719654890032No Hit
GCCTGTCTGAGCGTCGCT189100.23223643068694336No Hit
CTACGCCTGTCTGAGCGTCGCT184030.22600989074203162No Hit
TCAGTGCACTACAGAACTTTGT182730.22441334203820812No Hit
CAAAGAATTCTCCTTTTGGGCTT175380.2153867012896675No Hit
CTGGACTTGGAGTCAGAAGGC174480.2142813983408666No Hit
TGAGGTAGTAGTTTGTACAGTT172880.2123164153207761No Hit
GGAATACCGGGTGCTGTAGGCTTT172500.21184973185350459No Hit
TATTGCACTTGTCCCGGCCTGTAG169940.20870575902135988No Hit
AACGGAATCCCAAAAGCAGCTG168430.20685130629614945No Hit
TGTTTGTGATGACTTACA160810.1974930746629686No Hit
GGAATACCGGGTGCTGTAGGCTT157840.1938455749319256No Hit
CCGGGTGCTGTAGGCTTT155340.19077528896303425No Hit
CAAAGTGCTGTTCGTGCAGGTAG151840.18647688860658632No Hit
AAAAGCTGGGTTGAGAGGGCGA144370.17730287413153892No Hit
TCCTGTACTGAGCTGCCCCGAG144280.17719234383665883No Hit
TCCTGTACTGAGCTGCCCCGAGT140260.1722553239986815No Hit
TCGACCGGACCTCGACCGGCT139950.17187460853853898No Hit
TTCACAGTGGCTAAGTTCTGC136860.16807973508098922No Hit
TGTAAACATCCTTGACTGGAAGC135540.1664586240894146No Hit
TCAGGCTCAGTCCCCTCCCGAT128760.1581320085417812No Hit
CCCCCCACTGCTAAATTTGACTGGCT128340.15761620049900743No Hit
TTCACAGTGGCTAAGTTCCG128310.15757935706738074No Hit
GTAAACATCCTTGACTGGAAGCT125720.1543985408036093No Hit
CCCCCACTGCTAAATTTGACTGGCTT125390.15399326305571562No Hit
TACGCCTGTCTGAGCGTCGCT123180.15127913025921566No Hit
TGAGACCTCTGGGTTCTGAGCT117310.1440700988042587No Hit
TGTAAACATCCCCGACTGGAA115930.14237530094943066No Hit
TCAGTGCACTACAGAACTTTGTC115740.14214195921579492No Hit
ACTGGACTTGGAGTCAGAAGGCA115310.1416138700291456No Hit
CATTGCACTTGTCTCGGTCTGA115290.14158930774139447No Hit
TTCAAGTAATTCAGGATAGGTT111700.13718037709006647No Hit
GAATACCGGGTGCTGTAGGCTTT110760.1360259495657633No Hit
AGCTCGGTCTGAGGCCCCTCAGT110370.13554698495461626No Hit
ATCCCACCGCTGCCACCA106680.1310152428645326No Hit
ATCCCGGACGAGCCCCCA104500.12833795349965932No Hit
GCGTTCGCGCTTTCCCCTG104270.12805548719052132No Hit
CAACGGAATCCCAAAAGCAGCT101750.1249606389338788No Hit
TCAGGCTCAGTCCCCTCCCGA101600.12477642177574533No Hit
TTCAAGTAATCCAGGATAGGCTT101020.12406411543096255No Hit
TAGCTTATCAGACTGATGTTGACA100680.1236465565391933No Hit
CCCCCCACTGCTAAATTTGACTGG100040.12286056333115712No Hit
GGGAATACCGGGTGCTGTAGGCTT96700.11875866127671825No Hit
AAAAGCTGGGTTGAGAGGGCGAA96550.11857444411858477No Hit
ACGCCTGTCTGAGCGTCGCT95840.11770248290341961No Hit
TCCTGTACTGAGCTGCCCCGAGA95440.117211237148397No Hit
TATTGCACTTGTCCCGGCCTGTT95130.11683052168825446No Hit
TTCACAGTGGCTAAGTTCTG94670.11626558906997844No Hit
CAACACCAGTCGATGGGCTGTC94090.11555328272519565No Hit
TGAGATGAAGCACTGTAGCT93430.11474272722940833No Hit
GAATACCGGGTGCTGTAGGCTT89200.10954780337004413No Hit
TGTAAACATCCCCGACTGGAAG87700.1077056317887093No Hit
TTCTCACTACTGCACTTGACTAG84690.10400900748216409No Hit
TGAGGTAGTAGATTGTATAGTT83460.10249842678546955No Hit
CAAAGTGCTGTTCGTGCAGGT83260.10225280390795823No Hit
GGGAATACCGGGTGCTGTAGGCTTT82400.1011966255346596No Hit
TCCCCGGCACCTCCACCA82140.10087731579389489No Hit
ACTGGACTTGGAGTCAGAAGGA81970.10066853634801028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)