FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8470705
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7654139.03600113567879No Hit
CCCCCCACTGCTAAATTTGACTG3944024.656070539583187No Hit
AAGCTGCCAGTTGAAGAACTGT3424924.043252598219393No Hit
AACATTCAACGCTGTCGGTGAGT3176713.7502309429970704No Hit
TAGCAGCACGTAAATATTGGCG2840903.3537940466584537No Hit
TTCAAGTAATCCAGGATAGGCT2553263.014223727540978No Hit
AACATTCAACGCTGTCGGTGAG2085832.462404250885847No Hit
TAGCTTATCAGACTGATGTTGACT1602031.8912593461819294No Hit
TATTGCACTTGTCCCGGCCTGTAT1592401.8798907528948299No Hit
TAGCTTATCAGACTGATGTTGAC1559111.8405906001920738No Hit
TATTGCACTTGTCCCGGCCTGTAA1524281.7994724169948073No Hit
TATTGCACTTGTCCCGGCCTGT1382111.6316351472516162No Hit
TAGCTTATCAGACTGATGTTGA1379661.6287428260103498No Hit
CCCCCACTGCTAAATTTGACTG1296441.53049834694987No Hit
TGAGGTAGTAGGTTGTATAGTT1182861.3964126952833322No Hit
ATCCCGGACGAGCCCCCA1160191.3696498697570036No Hit
CATAAAGTAGAAAGCACTACT1002561.1835614627117814No Hit
TCGTACGACTCTTAGCGG856321.0109193980902416No Hit
TATTGCACTTGTCCCGGCCTGTA791140.9339718476797386No Hit
TGAGGTAGTAGATTGTATAGTT725250.8561861143789093No Hit
CGCCTGTCTGAGCGTCGCT656100.774551823018273No Hit
TGTAAACATCCTTGACTGGAAGCT633900.7483438509545546No Hit
AGCAGCATTGTACAGGGCTATGA566760.6690824435510385No Hit
GCCTGTCTGAGCGTCGCT533850.6302308957754992No Hit
CCCATAAAGTAGAAAGCACTAC515730.6088395239829507No Hit
ACTGGACTTGGAGTCAGAAGGC502380.5930793245662551No Hit
CAACACCAGTCGATGGGCTGT500400.5907418567875992No Hit
CCCATAAAGTAGAAAGCACT468030.5525278002244205No Hit
TTCAAGTAATTCAGGATAGGTT465770.5498597814467627No Hit
CAACGGAATCCCAAAAGCAGCTGT436620.5154470613721054No Hit
TGTAAACATCCCCGACTGGAAGCT420320.49620427107306886No Hit
CATTGCACTTGTCTCGGTCTGA398930.47095253582789154No Hit
CACCCGGCTGTGTGCACATGTGC357190.4216768261909723No Hit
TGAGGTAGTAGTTTGTACAGTT351920.41545538417404454No Hit
AGCTCGGTCTGAGGCCCCTCAGT240210.28357734096512627No Hit
CACTAGATTGTGAGCTCCTGGA234150.27642327291530044No Hit
TATTGCACTTGTCCCGGCCTGTT223160.2634491462044777No Hit
ACCGGGTGCTGTAGGCTT221620.26163111570996744No Hit
TGTAAACATCCTACACTCTCAGCT213890.252505547058952No Hit
TGTCAGTTTGTCAAATACCCCAA210960.24904656696225405No Hit
ACCGGGTGCTGTAGGCTTA209820.2477007521806036No Hit
AACGGAATCCCAAAAGCAGCTG206310.24355705930025895No Hit
ATCCCACCGCTGCCACCA200940.23721756335511623No Hit
TATTGCACTTGTCCCGGCCTGTAG199690.2357418892524294No Hit
AACATTCAACGCTGTCGGTGAGTT196070.2314683370510483No Hit
TGAGAACTGAATTCCATAGGCTGT187040.2208080673332385No Hit
TGAGGTAGTAGTTTGTGCTGTT186560.22024140847780677No Hit
CACTAGATTGTGAGCTCCTGGAA181340.21407899342498646No Hit
TGAGAACTGAATTCCATGGGTT174900.20647632044794384No Hit
TAGCTTATCAGACTGATGTTGACA161560.19072792642406977No Hit
TTCTCACTACTGCACTTGACTAG156230.18443565205021306No Hit
AACCGGGCGGAAACACCA154460.1823460975208085No Hit
ACCGGGTGCTGTAGGCTTT152930.18053987241911978No Hit
ACCACAGGGTAGAACCACGGAC150850.17808435071224885No Hit
TGAGGTAGTAGGTTGTGTGGTT149380.17634895796748912No Hit
TTCAAGTAATCCAGGATAGGCTT147600.17424759804526305No Hit
TAGCAGCACGTAAATATTGGC147370.1739760740103687No Hit
TACGCCTGTCTGAGCGTCGCT144120.17013932134338286No Hit
TTCAAGTAATCCAGGATAGGC136590.16124986054879728No Hit
TTATAAAGCAATGAGACTGATT135270.15969154869636No Hit
CTACGCCTGTCTGAGCGTCGCT134880.1592311383763217No Hit
ACGCCTGTCTGAGCGTCGCT130330.15385968464254157No Hit
AGCAGCATTGTACAGGGCTATGT130330.15385968464254157No Hit
ACTGGACTTGGAGTCAGAAGGCT129110.1524194267183192No Hit
TCGTTTCCCGGGCGGCGCACCA128090.15121527665052673No Hit
TGTCAGTTTGTCAAATACCCCA127560.1505895908309875No Hit
TATTGCACTTGTCCCGGCCTGTTT127560.1505895908309875No Hit
TTCACAGTGGCTAAGTTCCGC125790.14850003630158293No Hit
TTCACAGTGGCTAAGTTCCG125400.14803962598154463No Hit
ACTGGACTTGGAGTCAGAAGGCA122510.14462786745613262No Hit
TTCACCACCTTCTCCACCCAGC122100.14414384635045135No Hit
CCCATAAAGTAGAAAGCACTA120610.14238484282004862No Hit
CTCCCACTGCTTCACTTGACTAG120400.14213692957079724No Hit
TGAGGTAGTAGGTTGTATAGT118060.13937446765056746No Hit
ACTGGACTTGGAGTCAGAAGG117460.13866614408127778No Hit
CAACGGAATCCCAAAAGCAGCT116360.13736755087091335No Hit
CTTCTCACTACTGCACTTGACTAG115760.13665922730162366No Hit
TGTAAACATCCCCGACTGGAAGC115050.13582104441129753No Hit
TTAATGCTAATCGTGATAGGGGTT113570.1340738462737163No Hit
TCCTGTCTGAGCGTCGCT113240.13368426831060698No Hit
TATTGCACTTGTCCCGGCCTGA111420.1315356868170949No Hit
TCAGTGCACTACAGAACTTTGT111350.13145304906734445No Hit
CAAAGAATTCTCCTTTTGGGCT110720.1307093093195903No Hit
TATTGCACTTGTCCCGGCCTGTAAT109760.12957599160872676No Hit
AGCAGCATTGTACAGGGCTATG109560.12933988375229688No Hit
CAAAGTGCTGTTCGTGCAGGT106610.12585729286995592No Hit
CAAAGTGCTGTTCGTGCAGGTAG106040.12518438547913072No Hit
TGATGTAGTAGGTTGTATAGTT104670.12356704666258594No Hit
AGAGGTAGTAGGTTGCATAGTT101350.11964765624584967No Hit
TTCACAGTGGCTAAGTTCTG96610.11405190004846115No Hit
TTCACAGTGGCTAAGTTCTGC96420.11382759758485274No Hit
CAAAGAATTCTCCTTTTGGGCTT94410.11145471362773228No Hit
AACATTCAACGCTGTCGGTGA94010.1109824979148725No Hit
GGAATACCGGGTGCTGTAGGCTTT93270.11010889884608187No Hit
AATGACACGATCACTCCCGTTG89590.1057645142877718No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)