FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8034827
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7664419.538985718049684No Hit
CCCCCCACTGCTAAATTTGACTG4658215.797523705239702No Hit
AACATTCAACGCTGTCGGTGAGT3030603.7718298103991534No Hit
TAGCAGCACGTAAATATTGGCG2901023.6105568918907647No Hit
AAGCTGCCAGTTGAAGAACTGT2727563.3946717209966066No Hit
TTCAAGTAATCCAGGATAGGCT2285812.8448776806271No Hit
AACATTCAACGCTGTCGGTGAG2003202.493146398796141No Hit
TAGCTTATCAGACTGATGTTGACT1976942.4604636789317307No Hit
TATTGCACTTGTCCCGGCCTGTAT1872692.3307160191501324No Hit
TATTGCACTTGTCCCGGCCTGTAA1859962.3148724919652905No Hit
TAGCTTATCAGACTGATGTTGAC1686002.0983650301369274No Hit
CCCCCACTGCTAAATTTGACTG1256981.564414516952263No Hit
TAGCTTATCAGACTGATGTTGA1246801.5517446735318634No Hit
TATTGCACTTGTCCCGGCCTGT1117141.390372188474002No Hit
TGAGGTAGTAGGTTGTATAGTT886561.1033965012563431No Hit
TATTGCACTTGTCCCGGCCTGTA759520.9452848206937126No Hit
TGTAAACATCCTTGACTGGAAGCT680690.8471744320070612No Hit
CATAAAGTAGAAAGCACTACT646120.8041492368161753No Hit
ATCCCGGACGAGCCCCCA580180.7220815084133112No Hit
AGCAGCATTGTACAGGGCTATGA564930.7031016349200798No Hit
TGAGGTAGTAGATTGTATAGTT531590.6616072754273364No Hit
ACTGGACTTGGAGTCAGAAGGC510650.6355457311028601No Hit
TGTAAACATCCCCGACTGGAAGCT486580.6055886455302647No Hit
CCCATAAAGTAGAAAGCACTAC478770.5958684611379934No Hit
CAACGGAATCCCAAAAGCAGCTGT464010.5774984327602822No Hit
TTCAAGTAATTCAGGATAGGTT442440.5506528018587084No Hit
TTAATGCTAATCGTGATAGGGGTT392690.4887348539053797No Hit
CGCCTGTCTGAGCGTCGCT364640.453824332496518No Hit
TCGTACGACTCTTAGCGG357240.44461442667029416No Hit
CACCCGGCTGTGTGCACATGTGC341360.4248504665999654No Hit
CAACACCAGTCGATGGGCTGT316590.39402217371948395No Hit
TGAGAACTGAATTCCATAGGCTGT311550.3877494811027045No Hit
CCCATAAAGTAGAAAGCACT298820.3719059539178628No Hit
CATTGCACTTGTCTCGGTCTGA294390.3663924562408126No Hit
CACTAGATTGTGAGCTCCTGGA252220.3139084388500213No Hit
TATTGCACTTGTCCCGGCCTGTAG248790.30963952304137976No Hit
TGAGGTAGTAGTTTGTACAGTT237540.2956379770217828No Hit
CACTAGATTGTGAGCTCCTGGAA232580.28946485095447605No Hit
TAGCTTATCAGACTGATGTTGACA232140.28891723493237625No Hit
TGAGAACTGAATTCCATGGGTT229720.2859053468108274No Hit
GCCTGTCTGAGCGTCGCT217510.2707090021975582No Hit
TATTGCACTTGTCCCGGCCTGTT210100.26148665055265036No Hit
AGCTCGGTCTGAGGCCCCTCAGT209610.26087680543713015No Hit
AACATTCAACGCTGTCGGTGAGTT203440.25319773530904893No Hit
TGTAAACATCCTACACTCTCAGCT196330.2443487582246637No Hit
TCGTTTCCCGGGCGGCGCACCA179490.22338999956066258No Hit
TGTCAGTTTGTCAAATACCCCAA175130.21796362261440103No Hit
AACGGAATCCCAAAAGCAGCTG171330.21323421151444832No Hit
CAAAGAATTCTCCTTTTGGGCT171020.21284839113524162No Hit
TATTGCACTTGTCCCGGCCTGTTT160860.20020343935221005No Hit
TAGCAGCACGTAAATATTGGC158650.197452913423027No Hit
TTCTCACTACTGCACTTGACTAG157650.19620833155461842No Hit
TATTGCACTTGTCCCGGCCTGTAAT157020.19542424497752098No Hit
ACTGGACTTGGAGTCAGAAGGCT144710.18010344217741092No Hit
TTCAAGTAATCCAGGATAGGCTT144700.18009099635872683No Hit
ACTGGACTTGGAGTCAGAAGGCA141680.1763323591161328No Hit
CAAAGAATTCTCCTTTTGGGCTT139320.17339514590668847No Hit
TGAGGTAGTAGTTTGTGCTGTT137430.17104288617539617No Hit
ACCGGGTGCTGTAGGCTTA131160.16323935786047417No Hit
TATTGCACTTGTCCCGGCCTGTAAA130050.16185787198654059No Hit
CTTCTCACTACTGCACTTGACTAG125540.15624480776001773No Hit
CCCCCACTGCTAAATTTGACTGGCTT124830.15536115463344763No Hit
TGTAAACATCCCCGACTGGAAGC123880.15417880185845942No Hit
AGCAGCATTGTACAGGGCTATGT123710.15396722294082996No Hit
CTACGCCTGTCTGAGCGTCGCT118270.14719669757668707No Hit
ACCACAGGGTAGAACCACGGAC117800.14661174409853503No Hit
ACCGGGTGCTGTAGGCTT117410.14612635716985567No Hit
GGAATACCGGGTGCTGTAGGCTTT114720.1427784319438365No Hit
TTCAAGTAATCCAGGATAGGC112390.13987855619044443No Hit
TACGCCTGTCTGAGCGTCGCT110550.13758852555257256No Hit
TGAGGTAGTAGGTTGTGTGGTT109980.13687911388757965No Hit
CAACGGAATCCCAAAAGCAGCT109000.1356594236565392No Hit
TGTCAGTTTGTCAAATACCCCA107090.13328227228787876No Hit
TTCACCACCTTCTCCACCCAGC106770.132884006089988No Hit
AGCTACATCTGGCTACTGGGTCTC106560.13262264389762218No Hit
CAAAGTGCTGTTCGTGCAGGTAG106040.1319754613260497No Hit
TTATAAAGCAATGAGACTGATT104480.13003391361133226No Hit
ACTGGACTTGGAGTCAGAAGG102460.12751985823714687No Hit
ACCCCCCACTGCTAAATTTGACTG102040.12699713385241523No Hit
TGATGTAGTAGGTTGTATAGTT99980.1244332952034935No Hit
CCCCCCACTGCTAAATTTGACTGGCT99850.12427149956060037No Hit
TTCACAGTGGCTAAGTTCCGC98490.12257886821956465No Hit
CCCCCCACTGCTAAATTTGACTGG97500.12134673216984011No Hit
CCCCCCACTGCTAAATTTGACTGT95260.11855886878460481No Hit
CCCATAAAGTAGAAAGCACTA94400.1174885283777734No Hit
GGAATACCGGGTGCTGTAGGCTT92890.1156092097564764No Hit
TATTGCACTTGTCCCGGCCTGA92790.11548475156963553No Hit
ACCGGGTGCTGTAGGCTTT91710.11414060315175423No Hit
AACCGGGCGGAAACACCA91110.11339385403070906No Hit
CTCCCACTGCTTCACTTGACTAG91020.11328184166255227No Hit
CAAAGTGCTGTTCGTGCAGGT90910.11314493765702734No Hit
ATCCCACCGCTGCCACCA90720.1129084671020297No Hit
TTCACAGTGGCTAAGTTCCG89480.111365185585203No Hit
AGCAGCATTGTACAGGGCTATG89390.11125317321704624No Hit
ACGCCTGTCTGAGCGTCGCT83660.10412171911106487No Hit
TTCACAGTGGCTAAGTTCTGC83040.10335007835265153No Hit
TCAGTGCACTACAGAACTTTGT82070.10214283394029518No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)