FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13505650
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG13140539.729653885596028No Hit
CCCCCCACTGCTAAATTTGACTG11351388.40491201830345No Hit
TTCAAGTAATCCAGGATAGGCT4168353.086374961590149No Hit
AAGCTGCCAGTTGAAGAACTGT3686352.7294872886532673No Hit
AACATTCAACGCTGTCGGTGAGT3440182.547215424655607No Hit
TAGCAGCACGTAAATATTGGCG3404252.5206117439738183No Hit
CCCCCACTGCTAAATTTGACTG2867652.123296546260269No Hit
AACATTCAACGCTGTCGGTGAG2651381.9631635648784027No Hit
TAGCTTATCAGACTGATGTTGACT2628091.9459189302254982No Hit
TATTGCACTTGTCCCGGCCTGTAA2614961.9361970730768234No Hit
TAGCTTATCAGACTGATGTTGAC2585851.9146431308378347No Hit
TATTGCACTTGTCCCGGCCTGTAT2413371.7869336166715413No Hit
TTAATGCTAATCGTGATAGGGGTT1816331.3448667779781054No Hit
TAGCTTATCAGACTGATGTTGA1794761.3288956843987516No Hit
TGAGGTAGTAGGTTGTATAGTT1760691.3036692051104537No Hit
TATTGCACTTGTCCCGGCCTGT1268020.9388811349324172No Hit
TCGTACGACTCTTAGCGG1267610.9385775582811638No Hit
TGTAAACATCCTTGACTGGAAGCT1128650.8356872864319748No Hit
TATTGCACTTGTCCCGGCCTGTA1124900.8329106707192916No Hit
TGAGGTAGTAGATTGTATAGTT1013960.7507672714752714No Hit
TTCAAGTAATTCAGGATAGGTT998330.7391943371848078No Hit
AGCAGCATTGTACAGGGCTATGA884170.6546667505821638No Hit
CAACGGAATCCCAAAAGCAGCTGT869570.6438564600741171No Hit
TGTAAACATCCCCGACTGGAAGCT695950.5153028547311681No Hit
CATAAAGTAGAAAGCACTACT632520.46833732548970247No Hit
CAACGGAATCCCAAAAGCAGCT584680.43291511330443183No Hit
CCCATAAAGTAGAAAGCACTAC583710.4321968953734178No Hit
ACTGGACTTGGAGTCAGAAGGC577640.4277024800731546No Hit
TGAGAACTGAATTCCATAGGCTGT561970.4160999285484223No Hit
CAAAGAATTCTCCTTTTGGGCT469080.3473213062681174No Hit
CACCCGGCTGTGTGCACATGTGC448680.33221651679112074No Hit
TAGCTTATCAGACTGATGTTGACA440420.32610055791465054No Hit
TGAGGTAGTAGTTTGTACAGTT418980.31022572034666973No Hit
TGTAAACATCCTACACTCTCAGCT392800.2908412405178573No Hit
CATTGCACTTGTCTCGGTCTGA387120.28663559325171317No Hit
TTCTCACTACTGCACTTGACTAG372010.2754476830067416No Hit
CCCCCACTGCTAAATTTGACTGGCTT366890.2716566770203582No Hit
CCCATAAAGTAGAAAGCACT359040.2658442947951413No Hit
TTAATGCTAATCGTGATAGGGGTTT338890.2509246130323235No Hit
TCGTTTCCCGGCCAATGCACCA327370.24239484956296067No Hit
TGAGAACTGAATTCCATGGGTT326910.24205425136887157No Hit
CACTAGATTGTGAGCTCCTGGAA311260.23046650846127362No Hit
TATTGCACTTGTCCCGGCCTGTAG309160.22891160366217103No Hit
ACCCCCCACTGCTAAATTTGACTG304880.22574255959542858No Hit
CTTCTCACTACTGCACTTGACTAG296650.21964881364465982No Hit
TATTGCACTTGTCCCGGCCTGTT291320.21570231717836608No Hit
TCGTTTCCCGGGCGGCGCACCA285100.21109683724959555No Hit
AACGGAATCCCAAAAGCAGCTG282960.20951231521622432No Hit
CACTAGATTGTGAGCTCCTGGA279930.20726880972037628No Hit
AACATTCAACGCTGTCGGTGAGTT269330.19942024263919175No Hit
AGCTCGGTCTGAGGCCCCTCAGT268120.19852432130256595No Hit
CAACACCAGTCGATGGGCTGT257020.19030553879302367No Hit
TGAGGTAGTAGTTTGTGCTGTT252370.18686253530929647No Hit
TATTGCACTTGTCCCGGCCTGTAAT232120.17186881046080715No Hit
CCCCCCACTGCTAAATTTGACTGGCT229640.17003254193615266No Hit
CTCCCACTGCTTCACTTGACTAG228710.16934394123940721No Hit
TATTGCACTTGTCCCGGCCTGTAAA226970.1680555915487222No Hit
TGAGGTAGTAGGTTGTGTGGTT223220.16527897583603898No Hit
TATTGCACTTGTCCCGGCCTGTTT218630.16188039820371475No Hit
TAGCAGCACGTAAATATTGGC218190.1615546086267599No Hit
CAAAGAATTCTCCTTTTGGGCTT213820.15831892578291307No Hit
TTAATGCTAATCGTGATAGGGGT205110.15186977302092086No Hit
TTCAAGTAATCCAGGATAGGCTT202200.1497151192278787No Hit
TTCAAGTAATCCAGGATAGGC195610.1448356798821234No Hit
CCCCCCACTGCTAAATTTGACTGT194850.14427295243101962No Hit
AGCAGCATTGTACAGGGCTATGT192750.14271804763191703No Hit
CCCCCCACTGCTAAATTTGACTGG189940.1406374369245464No Hit
TTCACAGTGGCTAAGTTCCG180550.13368479117998763No Hit
CAAAGTGCTGTTCGTGCAGGTAG175910.1302491920048276No Hit
TGATGTAGTAGGTTGTATAGTT175420.129886380885037No Hit
CTACGCCTGTCTGAGCGTCGCT170430.12619163091002655No Hit
AGCTACATCTGGCTACTGGGTCTC167870.12429612791683481No Hit
ATCCCGGACGAGCCCCCA167800.12424429775686471No Hit
TACGCCTGTCTGAGCGTCGCT167720.12418506328832747No Hit
AGCTACATTGTCTGCTGGGTTT167030.12367416599719377No Hit
CCCATAAAGTAGAAAGCACTA166930.1236001229115222No Hit
TGAGGTAGTAGGTTGTATAGT159150.11783957084627544No Hit
ACTGGACTTGGAGTCAGAAGGCA157890.11690662796681389No Hit
TGTCAGTTTGTCAAATACCCCA155700.11528508439060689No Hit
TGTAAACATCCCCGACTGGAAGC150550.11147186547852195No Hit
CAACGGAATCCCAAAAGCAGCTGA149340.11057594414189617No Hit
CGCCTGTCTGAGCGTCGCT148820.1101909200964041No Hit
CCCCCCACTGCTAAATTTGACTGTA148420.1098947477537179No Hit
CACCCGTAGAACCGACCTTGCG143930.1065702132070652No Hit
ACCGGGTGCTGTAGGCTT142560.10555582293336493No Hit
ATGCACCTGGGCAAGGATTCTGA140810.10426006893411277No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)