FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10649441
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG10066259.452374072967773No Hit
TTCAAGTAATCCAGGATAGGCT4985744.681691743256758No Hit
CCCCCCACTGCTAAATTTGACTG4422544.152837693546544No Hit
AAGCTGCCAGTTGAAGAACTGT3831603.5979353282486843No Hit
TAGCAGCACGTAAATATTGGCG3475643.2636830421427754No Hit
AACATTCAACGCTGTCGGTGAGT3359693.154804087838977No Hit
AACATTCAACGCTGTCGGTGAG2361192.217196188983065No Hit
CCCCCACTGCTAAATTTGACTG1955561.8363029571223501No Hit
TGAGGTAGTAGGTTGTATAGTT1928181.810592687447163No Hit
CATAAAGTAGAAAGCACTACT1893391.7779243060739056No Hit
TAGCTTATCAGACTGATGTTGA1765851.6581621514218448No Hit
TAGCTTATCAGACTGATGTTGAC1654541.5536402333230448No Hit
TAGCTTATCAGACTGATGTTGACT1647941.5474427249280034No Hit
TATTGCACTTGTCCCGGCCTGTAA1211721.1378249806726943No Hit
TATTGCACTTGTCCCGGCCTGTAT1067411.002315520598687No Hit
TATTGCACTTGTCCCGGCCTGT994710.9340490266108803No Hit
TGAGGTAGTAGATTGTATAGTT942570.8850887102900518No Hit
CCCATAAAGTAGAAAGCACTAC842930.7915251138533937No Hit
CAACACCAGTCGATGGGCTGT715630.6719883231429706No Hit
TGTAAACATCCTTGACTGGAAGCT710220.6669082442918836No Hit
CCCATAAAGTAGAAAGCACT676210.6349722957289495No Hit
CAACGGAATCCCAAAAGCAGCT636770.5979374879864586No Hit
CGCCTGTCTGAGCGTCGCT636360.5975524912528273No Hit
CAACGGAATCCCAAAAGCAGCTGT631530.593017041927365No Hit
TATTGCACTTGTCCCGGCCTGTA572620.5376995844195015No Hit
TTCAAGTAATTCAGGATAGGTT566690.532131217028199No Hit
AGCAGCATTGTACAGGGCTATGA515230.4838094318753444No Hit
GCCTGTCTGAGCGTCGCT502590.4719402642824163No Hit
ACTGGACTTGGAGTCAGAAGGC452390.42480163982316066No Hit
TCGTACGACTCTTAGCGG442560.4155711083802427No Hit
TGAGGTAGTAGTTTGTACAGTT440330.4134771017558574No Hit
TGTAAACATCCCCGACTGGAAGCT411840.3867245238505946No Hit
CATTGCACTTGTCTCGGTCTGA403010.37843300883116776No Hit
TTCACAGTGGCTAAGTTCCG384920.36144620173021286No Hit
ATCCCGGACGAGCCCCCA378990.3558778343389104No Hit
TCGTTTCCCGGCCAATGCACCA367690.3452669487534604No Hit
CACCCGGCTGTGTGCACATGTGC343440.3224958004838No Hit
TGTAAACATCCTACACTCTCAGCT311590.29258812739560697No Hit
TTCTCACTACTGCACTTGACTAG302680.2842214910623008No Hit
AACGGAATCCCAAAAGCAGCTG284970.26759151020227256No Hit
TTCACAGTGGCTAAGTTCCGC282820.26557262489176664No Hit
CCCCCACTGCTAAATTTGACTGGCTT262930.24689558822852767No Hit
TTCAAGTAATCCAGGATAGGC262440.246435470181017No Hit
TGTCAGTTTGTCAAATACCCCAA249180.2339841124055244No Hit
TGAGGTAGTAGTTTGTGCTGTT243030.22820916140105382No Hit
TCCACCACGTTCCCGTGG238050.22353285961206787No Hit
TTCACAGTGGCTAAGTTCTGC230490.21643389545047484No Hit
ACCGGGTGCTGTAGGCTT227930.21403001340633748No Hit
TTCACAGTGGCTAAGTTCTG223500.20987017065027166No Hit
AACATTCAACGCTGTCGGTGAGTT220590.20713763285791245No Hit
CCCATAAAGTAGAAAGCACTA207580.1949210291882926No Hit
TGAGGTAGTAGGTTGTGTGGTT207130.1944984717977216No Hit
CAAAGAATTCTCCTTTTGGGCT204530.1920570290966446No Hit
CTACGCCTGTCTGAGCGTCGCT198970.18683609778203383No Hit
TAGCAGCACGTAAATATTGGC198960.1868267076177989No Hit
TGAGGTAGTAGGTTGTATAGT188650.17714544829160517No Hit
CTTCTCACTACTGCACTTGACTAG182470.17134232679442987No Hit
TAGCTTATCAGACTGATGTTGACA179150.16822479226843925No Hit
TGATGTAGTAGGTTGTATAGTT171120.16068449038780533No Hit
TAGCAGCACATCATGGTTTACA168460.15818670670131887No Hit
TATTGCACTTGTCCCGGCCTGTT166790.15661854927408866No Hit
ATCCCACCGCTGCCACCA160080.15031774907246304No Hit
TGTAAACATCCTACACTCAGCT159340.1496228769190796No Hit
ACGCCTGTCTGAGCGTCGCT157470.14786691620715114No Hit
TTCAAGTAATCCAGGATAGGCTT155620.1461297358236925No Hit
TACGCCTGTCTGAGCGTCGCT153960.14457096856069723No Hit
CTCCCACTGCTTCACTTGACTAG151450.14221403733773444No Hit
CAAAGTGCTGTTCGTGCAGGT150120.1409651454944912No Hit
CACTAGATTGTGAGCTCCTGGA149940.1407961225382628No Hit
AGAATTACTCTGAGACCT149880.14073978155285333No Hit
TTATAAAGCAATGAGACTGATT148080.1390495519905693No Hit
TGTCAGTTTGTCAAATACCCCA145810.13691798470924435No Hit
AGAGGTAGTAGGTTGCATAGTT145130.13627945354127038No Hit
CAACGGAATCCCAAAAGCAGCTGA140170.1316219320807543No Hit
ACTGGACTTGGAGTCAGAAGGCT139010.13053267302950455No Hit
ACTGGACTTGGAGTCAGAAGG138570.13011950580316844No Hit
ACCGGGTGCTGTAGGCTTA138040.12962182709871814No Hit
AACATTCAACGCTGTCGGTGA133410.12527418105795413No Hit
AGCTCGGTCTGAGGCCCCTCAGT132540.12445723676951681No Hit
ACCACAGGGTAGAACCACGGAC131700.12366846297378427No Hit
ATCACATTGCCAGGGATTTCC131530.12350883018179075No Hit
ACCGGGTGCTGTAGGCTTT129080.12120823994423745No Hit
AATGCACCCGGGCAAGGATTCT128650.12080446288213625No Hit
TATTGCACTTGTCCCGGCCTGTAG126090.11840058083799891No Hit
TCCTGTCTGAGCGTCGCT125330.11768692835614565No Hit
TGAGGTAGTAAGTTGTATTGTT123430.11590279715151246No Hit
CCCCCCACTGCTAAATTTGACTGGCT121350.11394964299065087No Hit
GTCAGGATGGCCGAGCGGTCTAAGG120710.11334867247961652No Hit
CAAAGAATTCTCCTTTTGGGCTT120390.11304818722409936No Hit
AATGACACGATCACTCCCGTTG119350.11207161014366858No Hit
TGTAAACATCCCCGACTGGAAGC118370.11115137404864725No Hit
CACTAGATTGTGAGCTCCTGGAA117370.1102123576251561No Hit
TGTCAGTTTGTCAAATACC113890.10694458047140691No Hit
CATAAAGTAGAAAGCACTAC113390.10647507225966134No Hit
TATTGCACTTGTCCCGGCCTGA113330.10641873127425186No Hit
AGCAGCATTGTACAGGGCTATG112870.10598678371944593No Hit
TCAGTGCACTACAGAACTTTGT109120.10246547213135412No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)