FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10674094
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8337087.8105738997614225No Hit
CCCCCCACTGCTAAATTTGACTG6906606.470432057278116No Hit
TTCAAGTAATCCAGGATAGGCT3814513.5736147723638187No Hit
TAGCAGCACGTAAATATTGGCG3378463.165102349670145No Hit
AAGCTGCCAGTTGAAGAACTGT3236643.0322386143498457No Hit
AACATTCAACGCTGTCGGTGAGT3196772.994886498095295No Hit
TAGCTTATCAGACTGATGTTGAC2483582.326736114559231No Hit
TAGCTTATCAGACTGATGTTGACT2451032.2962417231851244No Hit
AACATTCAACGCTGTCGGTGAG2227522.0868469024162613No Hit
CCCCCACTGCTAAATTTGACTG2108321.9751746611937275No Hit
TATTGCACTTGTCCCGGCCTGTAA2043211.9141765099689023No Hit
TATTGCACTTGTCCCGGCCTGTAT1921741.8003776245553018No Hit
TAGCTTATCAGACTGATGTTGA1698021.5907860657775732No Hit
TGTAAACATCCTTGACTGGAAGCT1264061.1842316546959395No Hit
TGAGGTAGTAGGTTGTATAGTT1192981.117640522933375No Hit
TTCAAGTAATTCAGGATAGGTT1161181.0878487673052157No Hit
CATAAAGTAGAAAGCACTACT1152031.0792766112046606No Hit
TATTGCACTTGTCCCGGCCTGT943790.8841874542232812No Hit
TGAGGTAGTAGATTGTATAGTT903990.846900917305019No Hit
TATTGCACTTGTCCCGGCCTGTA752880.7053338672115873No Hit
TGTAAACATCCCCGACTGGAAGCT724780.6790084479301007No Hit
AGCAGCATTGTACAGGGCTATGA662750.6208957875019651No Hit
TCGTACGACTCTTAGCGG591300.5539580221047332No Hit
CAACGGAATCCCAAAAGCAGCTGT536040.5021878203433472No Hit
CCCATAAAGTAGAAAGCACTAC483960.453396794144777No Hit
CACCCGGCTGTGTGCACATGTGC410750.3848101768637226No Hit
CATTGCACTTGTCTCGGTCTGA402800.3773622379566828No Hit
ACTGGACTTGGAGTCAGAAGGC399390.3741675874317764No Hit
CAAAGAATTCTCCTTTTGGGCT393100.368274815642433No Hit
TGAGGTAGTAGTTTGTACAGTT378180.354297048536391No Hit
CAACGGAATCCCAAAAGCAGCT357890.33528840948936744No Hit
TAGCTTATCAGACTGATGTTGACA345430.3236152876300321No Hit
TGATGTAGTAGGTTGTATAGTT328140.30741719156679714No Hit
TTCTCACTACTGCACTTGACTAG316350.29637175764050794No Hit
TGTAAACATCCTACACTCTCAGCT313220.29343942446075516No Hit
CAAAGAATTCTCCTTTTGGGCTT293230.27471183971211044No Hit
CTTCTCACTACTGCACTTGACTAG291830.27340025298634246No Hit
CCCATAAAGTAGAAAGCACT291170.2727819335299089No Hit
CCCCCACTGCTAAATTTGACTGGCTT264960.24822715632820924No Hit
CAACACCAGTCGATGGGCTGT257460.24120079886873771No Hit
TGTCAGTTTGTCAAATACCCCAA256890.24066679570181787No Hit
TGATGTAGTAGATTGTATAGTT244830.2293684129069877No Hit
CTCCCACTGCTTCACTTGACTAG238980.22388785408859993No Hit
AACATTCAACGCTGTCGGTGAGTT234510.2197001450427549No Hit
ACCCCCCACTGCTAAATTTGACTG232680.2179857138226439No Hit
TAGCAGCACGTAAATATTGGC231890.2172456041702462No Hit
TTATAAAGCAATGAGACTGATT227670.21329210703971688No Hit
TATTGCACTTGTCCCGGCCTGTAG220880.20693091141974201No Hit
TTCAAGTAATCCAGGATAGGCTT219050.205216480199631No Hit
TTCACAGTGGCTAAGTTCCGC212990.19953918337237803No Hit
TGTCAGTTTGTCAAATACCCCA208640.19546389604588454No Hit
CACTAGATTGTGAGCTCCTGGAA204040.19115439680407537No Hit
CACTAGATTGTGAGCTCCTGGA197800.18530846739779508No Hit
TATTGCACTTGTCCCGGCCTGTT194180.18191707886402347No Hit
TGTAAACATCCCCGACTGGAAGC191650.17954685428102846No Hit
TATTGCACTTGTCCCGGCCTGTAAT191070.179003482637496No Hit
TGAGGTAGTAGTTTGTGCTGTT175460.1643792906451826No Hit
TATTGCACTTGTCCCGGCCTGTTT167250.1566877713462145No Hit
CCCCCCACTGCTAAATTTGACTGGCT158860.148827619468219No Hit
AGCAGCATTGTACAGGGCTATGT156880.14697266109891857No Hit
TTCAAGTAATCCAGGATAGGC154840.14506149186994233No Hit
TATTGCACTTGTCCCGGCCTGTAAA149800.14033977965717748No Hit
AACGGAATCCCAAAAGCAGCTG149640.14018988403137542No Hit
CCCCCCACTGCTAAATTTGACTGG147370.1380632398403087No Hit
CGCGACCTCAGATCAGACGTGGCG143660.13458753501702347No Hit
TTTAAACATCCTTGACTGGAAGCT142590.13358510801947218No Hit
TCGTTTCCCGGCCAATGCACCA140700.13181446593968538No Hit
TTCACAGTGGCTAAGTTCTGC136320.127711073183354No Hit
TCAGTGCACTACAGAACTTTGT133140.12473189762053809No Hit
CTCCCACTGCTTCACTTGACTAGCCT132520.12415105207055513No Hit
ACTTGACTTGGAGTCAGAAGGC131040.12276451753188608No Hit
TAGCAGCACGTAAATATTGGCGT130190.12196819701981265No Hit
TTCACAGTGGCTAAGTTCCG126850.11883912583119467No Hit
ATGCACCTGGGCAAGGATTCTGA123730.11591616112805452No Hit
CAAAGTGCTGTTCGTGCAGGTAG122220.11450152115954758No Hit
ACTGGACTTGGAGTCAGAAGGCA121570.11389257017972673No Hit
CCCATAAAGTAGAAAGCACTA117840.11039812840321624No Hit
ACCGGGTGCTGTAGGCTTA116110.10877738194923148No Hit
ATCACATTGCCAGGGATTTCCA116090.10875864499600622No Hit
CTACGCCTGTCTGAGCGTCGCT115220.10794358753070751No Hit
AGCTCGGTCTGAGGCCCCTCAGT112010.10493630653805372No Hit
ATCACATTGCCAGGGATTTCC111640.10458967290338647No Hit
ACTGGACTTGGAGTCAGAAGGCT110440.10346545570987101No Hit
CGCCTGTCTGAGCGTCGCT110180.10322187531794268No Hit
ACCGGGTGCTGTAGGCTT109790.10285650473005016No Hit
TGAGAACTGAATTCCATAGGCTGT108830.1019571309752378No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)