FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12107005
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8200256.773144968553329No Hit
CCCCCCACTGCTAAATTTGACTG6033444.983429014855449No Hit
AACATTCAACGCTGTCGGTGAGT5189614.286452347215517No Hit
AAGCTGCCAGTTGAAGAACTGT3908393.2282054893014416No Hit
TAGCAGCACGTAAATATTGGCG3488452.88134844249259No Hit
AACATTCAACGCTGTCGGTGAG3355932.771891148967065No Hit
TTCAAGTAATCCAGGATAGGCT3096942.5579736689627204No Hit
TATTGCACTTGTCCCGGCCTGTAA2309871.9078789510700622No Hit
TATTGCACTTGTCCCGGCCTGTAT2280641.8837359033055656No Hit
TAGCTTATCAGACTGATGTTGACT2198101.8155604957625773No Hit
TAGCTTATCAGACTGATGTTGAC2127281.7570654344323804No Hit
CCCCCACTGCTAAATTTGACTG1974241.6306592753534008No Hit
TAGCTTATCAGACTGATGTTGA1632831.3486655039788948No Hit
TATTGCACTTGTCCCGGCCTGT1546221.2771284062408497No Hit
TGAGGTAGTAGGTTGTATAGTT1463821.2090686342328265No Hit
CATAAAGTAGAAAGCACTACT1152490.9519199835136766No Hit
TGAGGTAGTAGATTGTATAGTT1138740.940562922043891No Hit
TATTGCACTTGTCCCGGCCTGTA1065470.8800442388518052No Hit
TGTAAACATCCTTGACTGGAAGCT926530.7652842300800239No Hit
TTCAAGTAATTCAGGATAGGTT825070.6814815059546107No Hit
AGCAGCATTGTACAGGGCTATGA730750.6035761941124167No Hit
ACTGGACTTGGAGTCAGAAGGC643260.5313122444403054No Hit
TGTAAACATCCCCGACTGGAAGCT636530.5257534790809123No Hit
GCCTGTCTGAGCGTCGCT612410.5058311283426413No Hit
TCGTACGACTCTTAGCGG549260.45367124239231754No Hit
CATTGCACTTGTCTCGGTCTGA527410.4356238392566948No Hit
ATCCCGGACGAGCCCCCA500970.4137852425104309No Hit
CCCATAAAGTAGAAAGCACTAC494820.408705538653036No Hit
CACCCGGCTGTGTGCACATGTGC472300.3901047368857946No Hit
CGCCTGTCTGAGCGTCGCT465590.38456249088853933No Hit
ACCGGGTGCTGTAGGCTTA462420.381944171989687No Hit
TGAGGTAGTAGTTTGTACAGTT453660.3747086913733No Hit
CAACACCAGTCGATGGGCTGT442150.3652017984629559No Hit
CAACGGAATCCCAAAAGCAGCTGT425820.3517137392773853No Hit
ACCGGGTGCTGTAGGCTT415140.3428923998957628No Hit
CCCATAAAGTAGAAAGCACT365830.30216391254484487No Hit
AACATTCAACGCTGTCGGTGAGTT362500.2994134387488896No Hit
TTAATGCTAATCGTGATAGGGGTT354890.29312782145543015No Hit
ACCGGGTGCTGTAGGCTTT331520.2738249467973293No Hit
TCCTGTCTGAGCGTCGCT313640.25905663704607373No Hit
TGTCAGTTTGTCAAATACCCCAA306320.2530105505036134No Hit
CTCCCACTGCTTCACTTGACTAG296930.24525470997988358No Hit
TATTGCACTTGTCCCGGCCTGTAG293010.2420169150008611No Hit
CACTAGATTGTGAGCTCCTGGA291300.24060450953807322No Hit
TTCTCACTACTGCACTTGACTAG287990.23787055510425575No Hit
TGAGGTAGTAGTTTGTGCTGTT275490.22754595376808717No Hit
TATTGCACTTGTCCCGGCCTGTT274780.2269595164121928No Hit
TTATAAAGCAATGAGACTGATT260330.2150242772675819No Hit
CACTAGATTGTGAGCTCCTGGAA252710.2087304002930535No Hit
TGAGAACTGAATTCCATAGGCTGT250800.20715280120888693No Hit
TAGCTTATCAGACTGATGTTGACA245140.20247782172386977No Hit
TGATGTAGTAGGTTGTATAGTT244150.20166011329804523No Hit
TGAGAACTGAATTCCATGGGTT237650.19629132060323753No Hit
CAAAGAATTCTCCTTTTGGGCT225140.18595845958600002No Hit
TGTAAACATCCTACACTCTCAGCT223700.1847690655120734No Hit
CTTCTCACTACTGCACTTGACTAG208620.17231346646011958No Hit
CGCGACCTCAGATCAGACGTGGCG207250.1711818901536755No Hit
TTCACAGTGGCTAAGTTCCGC205730.1699264186311974No Hit
CCCCCACTGCTAAATTTGACTGGCTT203320.1679358354935841No Hit
TTCAAGTAATCCAGGATAGGCTT199420.16471455987669947No Hit
AACGGAATCCCAAAAGCAGCTG192050.15862717492889447No Hit
CAAAGAATTCTCCTTTTGGGCTT190470.15732214532000274No Hit
TGATGTAGTAGATTGTATAGTT190280.157165211379693No Hit
TATTGCACTTGTCCCGGCCTGTTT187930.1552241863284933No Hit
TGTCAGTTTGTCAAATACCCCA185980.153613548520051No Hit
CGCGACCTCAGATCAGACGTGG185310.15306014988843236No Hit
TAGCAGCACGTAAATATTGGC184090.1520524687980223No Hit
ACTGGACTTGGAGTCAGAAGGCA182740.1509374118537161No Hit
AGCTCGGTCTGAGGCCCCTCAGT175820.14522171255401314No Hit
TATTGCACTTGTCCCGGCCTGTAAT174460.144098395928638No Hit
TGTAAACATCCCCGACTGGAAGC173470.14328068750281345No Hit
TTCACAGTGGCTAAGTTCCG173160.14302463738967647No Hit
ACCCCCCACTGCTAAATTTGACTG173060.14294204057898713No Hit
ACTGGACTTGGAGTCAGAAGG160050.13219619550830286No Hit
TTCAAGTAATCCAGGATAGGC156010.12885928435645314No Hit
AACATTCAACGCTGTCGGTGA153330.1266456898299786No Hit
AGCAGCATTGTACAGGGCTATGT152910.12629878322508334No Hit
CCCCCCACTGCTAAATTTGACTGGCT150940.12467162605450316No Hit
ACTGGACTTGGAGTCAGAAGGCT148510.12266452355475198No Hit
CAAAGTGCTGTTCGTGCAGGTAG145880.1204922274336221No Hit
CTACGCCTGTCTGAGCGTCGCT145730.1203683322175881No Hit
CCCCCCACTGCTAAATTTGACTGG144330.1192119768679372No Hit
TCGTTTCCCGGGCGGCGCACCA140700.11621371263991383No Hit
TATTGCACTTGTCCCGGCCTGTAAA140410.11597418188891472No Hit
TGAGGTAGTAGGTTGTATAGT139800.1154703413437097No Hit
TACGCCTGTCTGAGCGTCGCT138190.11414053269161119No Hit
ACCACAGGGTAGAACCACGGAC135080.11157177187917243No Hit
TTCACAGTGGCTAAGTTCTGC134050.11072102472907212No Hit
TTCACAGTGGCTAAGTTCTG133310.11010980832997096No Hit
CAAAGTGCTGTTCGTGCAGGT131880.10892867393711328No Hit
TTAGGTAGTAGGTTGTATAGTT130750.10799532997632362No Hit
ACTTGACTTGGAGTCAGAAGGC129640.10707850537767184No Hit
AACCGGGCGGAAACACCA128010.10573217736343547No Hit
TATTGCACTTGTCCCGGCCTGA126850.10477405435943901No Hit
TCAGTGCACTACAGAACTTTGT124170.10256045983296448No Hit
CAACGGAATCCCAAAAGCAGCT123630.10211443705524198No Hit
TTTAAACATCCTTGACTGGAAGCT123360.10189142566638075No Hit
AGCAGCATTGTACAGGGCTATG122840.10146192225079613No Hit
AACATTCAACGCTGTCGGTGAGA121260.10015689264190442No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)