FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8443942
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7007698.29907405806435No Hit
CCCCCCACTGCTAAATTTGACTG4555815.395359181766052No Hit
TTCAAGTAATCCAGGATAGGCT3171163.755544507529777No Hit
TAGCAGCACGTAAATATTGGCG2830873.35254552909056No Hit
AAGCTGCCAGTTGAAGAACTGT2803793.3204751998533384No Hit
AACATTCAACGCTGTCGGTGAGT2289892.7118731985605775No Hit
TAGCTTATCAGACTGATGTTGAC2010032.380440320409591No Hit
AACATTCAACGCTGTCGGTGAG1727162.0454427564755893No Hit
CCCCCACTGCTAAATTTGACTG1513331.7922079521626273No Hit
TAGCTTATCAGACTGATGTTGACT1496651.7724541452321676No Hit
TATTGCACTTGTCCCGGCCTGTAA1416261.6772497963628836No Hit
TGAGGTAGTAGGTTGTATAGTT1367081.6190068572237943No Hit
TATTGCACTTGTCCCGGCCTGTAT1345241.5931421603795952No Hit
TAGCTTATCAGACTGATGTTGA1171801.387740465294527No Hit
TGAGGTAGTAGATTGTATAGTT978551.1588781637770604No Hit
TGTAAACATCCTTGACTGGAAGCT910031.077731230271359No Hit
TATTGCACTTGTCCCGGCCTGT899251.0649646811880045No Hit
CATAAAGTAGAAAGCACTACT805910.9544238934848203No Hit
TTCAAGTAATTCAGGATAGGTT689890.8170236129049678No Hit
TATTGCACTTGTCCCGGCCTGTA620290.7345976559289489No Hit
CAACGGAATCCCAAAAGCAGCT582100.6893699648813315No Hit
AGCAGCATTGTACAGGGCTATGA514150.6088980715405199No Hit
TGAGAACTGAATTCCATAGGCTGT512230.6066242520377331No Hit
TGTAAACATCCCCGACTGGAAGCT497300.5889429368415842No Hit
CAACGGAATCCCAAAAGCAGCTGT421300.4989375815229427No Hit
TGAGGTAGTAGTTTGTACAGTT418140.4951952535912729No Hit
ACTGGACTTGGAGTCAGAAGGC366620.43418109693316226No Hit
CCCATAAAGTAGAAAGCACTAC365990.43343499990881035No Hit
CACCCGGCTGTGTGCACATGTGC356690.42242118669218714No Hit
GCCTGTCTGAGCGTCGCT348830.4131127381026539No Hit
CACCCGTAGAACCGACCTTGCG342360.40545044009065906No Hit
CATTGCACTTGTCTCGGTCTGA339970.40262000852208607No Hit
TTAATGCTAATCGTGATAGGGGTT325540.3855308338214545No Hit
TACGCCTGTCTGAGCGTCGCT291200.344862624589321No Hit
TGAGAACTGAATTCCATGGGTT290430.3439507282262242No Hit
TGTAAACATCCTACACTCTCAGCT269290.31891502807574945No Hit
TTCACAGTGGCTAAGTTCCGC263750.3123541113854169No Hit
TAGCTTATCAGACTGATGTTGACA249560.2955491641226337No Hit
ACCGGGTGCTGTAGGCTT241010.28542356164928656No Hit
TGAGGTAGTAGTTTGTGCTGTT239900.284109009749238No Hit
CAAAGAATTCTCCTTTTGGGCT239090.28314974214649985No Hit
CCCATAAAGTAGAAAGCACT232180.27496636049844964No Hit
CAACACCAGTCGATGGGCTGT214430.25394537290758273No Hit
CTACGCCTGTCTGAGCGTCGCT206990.24513432233428414No Hit
ACCGGGTGCTGTAGGCTTA195960.2320717030031708No Hit
AACGGAATCCCAAAAGCAGCTG189920.22491864581732088No Hit
TTCTCACTACTGCACTTGACTAG187980.22262114069471345No Hit
TAGCAGCACGTAAATATTGGC181940.21546808350886354No Hit
ACCGGGTGCTGTAGGCTTT174700.20689388913377188No Hit
CCCCCACTGCTAAATTTGACTGGCTT174580.2067517754148477No Hit
TTCACAGTGGCTAAGTTCTGC169230.20041587211281175No Hit
CTCCCACTGCTTCACTTGACTAG165850.19641300236311426No Hit
TATTGCACTTGTCCCGGCCTGTT164100.19434051062880348No Hit
TCGTACGACTCTTAGCGG160270.18980471443314034No Hit
CGTTCGCGCTTTCCCCTG156670.18554130286541523No Hit
TATTGCACTTGTCCCGGCCTGTAG155640.18432149344464943No Hit
CACTAGATTGTGAGCTCCTGGA154020.18240295823917313No Hit
CAACGGAATCCCAAAAGCAGCTGA153400.18166870402473156No Hit
CAAAGAATTCTCCTTTTGGGCTT149520.17707369377951673No Hit
AACATTCAACGCTGTCGGTGAGTT148170.1754749144416198No Hit
TGTAAACATCCCCGACTGGAAGC147570.17476434584699896No Hit
CTTCTCACTACTGCACTTGACTAG142860.16918638237922526No Hit
ACCCCCCACTGCTAAATTTGACTG136190.16128722816902344No Hit
TGTCAGTTTGTCAAATACCCCAA135960.16101484354108545No Hit
TTCACAGTGGCTAAGTTCCG135790.16081351577260952No Hit
TTCAAGTAATCCAGGATAGGCTT135490.1604582314752991No Hit
CACTAGATTGTGAGCTCCTGGAA130300.1543118131318287No Hit
ATCCCGGACGAGCCCCCA130080.15405127131380106No Hit
TGTCAGTTTGTCAAATACCCCA126910.15029710057222087No Hit
TTCAAGTAATCCAGGATAGGC125160.14822460883791008No Hit
AGCTCGGTCTGAGGCCCCTCAGT123650.1464363445414476No Hit
CGCCTGTCTGAGCGTCGCT123020.1456902475170957No Hit
TAGCAGCACATCATGGTTTACA122610.14520469231077143No Hit
TATTGCACTTGTCCCGGCCTGTTT122240.14476650834408858No Hit
TCCTGTCTGAGCGTCGCT122140.1446480802449851No Hit
TTATAAAGCAATGAGACTGATT121050.14335721396475723No Hit
TCGTTTCCCGGCCAATGCACCA117720.1394135582646115No Hit
AGCAGCATTGTACAGGGCTATGT116050.13743580900958344No Hit
TCAGTGCACTACAGAACTTTGT112200.13287632719409964No Hit
ACTGGACTTGGAGTCAGAAGGCA111240.13173941744270626No Hit
TGTAAACATCCTACACTCAGCT110000.13027090901382318No Hit
TGAGGTAGTAGGTTGTGTGGTT107350.12713256438758105No Hit
TGAGGTAGTAGGTTGTATAGT107210.1269667650488362No Hit
TATTGCACTTGTCCCGGCCTGTAAT105600.12506007265327024No Hit
TATTGCACTTGTCCCGGCCTGA105470.12490611612443571No Hit
TGATGTAGTAGGTTGTATAGTT104630.12391132009196652No Hit
AATGCACCCGGGCAAGGATTCT103950.1231060090180629No Hit
ATCACATTGCCAGGGATTTCC102890.12185067116756605No Hit
ACTGGACTTGGAGTCAGAAGGCT102330.1211874738125866No Hit
TAGCAGCACGTAAATATTGGCGT98350.11647403546826826No Hit
CGCGACCTCAGATCAGACGTGG96950.11481604208081961No Hit
TATTGCACTTGTCCCGGCCTGTAAA96730.11455550026279195No Hit
CCCATAAAGTAGAAAGCACTA96550.11434232968440569No Hit
TGAGGTAGTAAGTTGTATTGTT96430.11420021596548152No Hit
ATGCACCTGGGCAAGGATTCTGA91000.1077695701841628No Hit
CAAAGTGCTGTTCGTGCAGGTAG87430.10354168704616873No Hit
ACTGGACTTGGAGTCAGAAGG84770.10039149961001627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)