FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9204553
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG7142317.759540305759552No Hit
CAACGGAATCCCAAAAGCAGCTG6025876.546618830919872No Hit
TAGCAGCACGTAAATATTGGCG3207453.4846341804974124No Hit
TTCAAGTAATCCAGGATAGGCT3138513.4097364641172687No Hit
AACATTCAACGCTGTCGGTGAGT2864713.1122749795671774No Hit
AAGCTGCCAGTTGAAGAACTGT2852473.0989772126902846No Hit
CCCCCACTGCTAAATTTGACTG2141492.3265551298362883No Hit
AACATTCAACGCTGTCGGTGAG1938732.1062728412775718No Hit
TAGCTTATCAGACTGATGTTGAC1823441.9810196106209612No Hit
TAGCTTATCAGACTGATGTTGACT1806211.9623006136202377No Hit
TATTGCACTTGTCCCGGCCTGTAA1450191.5755137701961193No Hit
TGAGGTAGTAGGTTGTATAGTT1367031.4851671775913506No Hit
TAGCTTATCAGACTGATGTTGA1328561.443372643951314No Hit
TATTGCACTTGTCCCGGCCTGTAT1319511.4335405532457686No Hit
TGTAAACATCCTTGACTGGAAGCT1050031.1407723981816391No Hit
TGAGGTAGTAGATTGTATAGTT937901.0189522511305003No Hit
CATAAAGTAGAAAGCACTACT854100.9279103504537374No Hit
TATTGCACTTGTCCCGGCCTGT792320.8607913931290309No Hit
TTCAAGTAATTCAGGATAGGTT726120.7888704644321131No Hit
TATTGCACTTGTCCCGGCCTGTA671520.7295519945400935No Hit
TGAGAACTGAATTCCATAGGCTGT665560.7230769381196458No Hit
TTAATGCTAATCGTGATAGGGGTT617880.6712764867560652No Hit
TGTAAACATCCCCGACTGGAAGCT598260.6499609486739878No Hit
AGCAGCATTGTACAGGGCTATGA557210.6053634543687239No Hit
CCCATAAAGTAGAAAGCACTAC408680.44399766072290525No Hit
ACTGGACTTGGAGTCAGAAGGC403760.4386524799194486No Hit
TGAGGTAGTAGTTTGTACAGTT378150.41082929285104886No Hit
CAACGGAATCCCAAAAGCAGCTGT363530.3949458490814274No Hit
CAAAGAATTCTCCTTTTGGGCT342590.3721962381008616No Hit
CAACGGAATCCCAAAAGCAGCT327200.35547625180712195No Hit
TCGTACGACTCTTAGCGG316650.3440145328078398No Hit
CCCCCACTGCTAAATTTGACTGGCTT298670.32448072166024794No Hit
TGTAAACATCCTACACTCTCAGCT295520.3210585022434006No Hit
CATTGCACTTGTCTCGGTCTGA276360.30024271683806913No Hit
ACCGGGTGCTGTAGGCTT271710.2951908691274851No Hit
CACCCGGCTGTGTGCACATGTGC269000.2922466740101339No Hit
ACCGGGTGCTGTAGGCTTA264200.2870318634701761No Hit
CCCATAAAGTAGAAAGCACT260210.2826970522088362No Hit
TTCTCACTACTGCACTTGACTAG259430.2818496454960931No Hit
TTCACAGTGGCTAAGTTCCG242730.26370645049249No Hit
TGTCAGTTTGTCAAATACCCCAA239860.26058842835714024No Hit
TAGCTTATCAGACTGATGTTGACA238680.2593064540994006No Hit
AACATTCAACGCTGTCGGTGAGTT237460.2579810230871613No Hit
TGAGAACTGAATTCCATGGGTT234480.25474349487693754No Hit
ACCGGGTGCTGTAGGCTTT229920.24978942486397765No Hit
CAACACCAGTCGATGGGCTGT226520.24609560073150755No Hit
TGAGGTAGTAGTTTGTGCTGTT216720.23544869587909376No Hit
CTTCTCACTACTGCACTTGACTAG201370.21877216633985375No Hit
ACCCCCCACTGCTAAATTTGACTG199060.21626253876749907No Hit
TAGCAGCACGTAAATATTGGC193340.2100482228740494No Hit
CAAAGAATTCTCCTTTTGGGCTT189280.20563736229233512No Hit
CTCCCACTGCTTCACTTGACTAG176760.19203539813394524No Hit
TATTGCACTTGTCCCGGCCTGTAG172640.18755935242048147No Hit
TATTGCACTTGTCCCGGCCTGTT172160.1870378713664857No Hit
CACTAGATTGTGAGCTCCTGGA171140.1859297241267447No Hit
CACTAGATTGTGAGCTCCTGGAA169570.18422404651263347No Hit
TGTCAGTTTGTCAAATACCCCA168360.18290947968901913No Hit
AACGGAATCCCAAAAGCAGCTG166600.18099738249103461No Hit
TGTAAACATCCCCGACTGGAAGC160550.1744245483729628No Hit
TTATAAAGCAATGAGACTGATT150710.16373418676604937No Hit
CACCCGTAGAACCGACCTTGCG150650.1636690016342999No Hit
CCCCCCACTGCTAAATTTGACTGGCT145300.15785666071997195No Hit
ACTGGACTTGGAGTCAGAAGGCA145030.15756332762709932No Hit
TCGTTTCCCGGCCAATGCACCA140730.15289172651838714No Hit
CCCCCCACTGCTAAATTTGACTGG138030.1499583955896609No Hit
TTCAAGTAATCCAGGATAGGCTT136890.14871987808642093No Hit
TTCAAGTAATCCAGGATAGGC132200.14362457362133718No Hit
CCCATAAAGTAGAAAGCACTA132020.14342901822608875No Hit
TTAATGCTAATCGTGATAGGGGTTT131460.1428206236630937No Hit
CGCCTGTCTGAGCGTCGCT123790.13448779098778615No Hit
ACTGGACTTGGAGTCAGAAGGCT119580.12991396757669818No Hit
TATTGCACTTGTCCCGGCCTGTTT118620.12887100546870664No Hit
CGCGACCTCAGATCAGACGTGGCG117680.12784977173796488No Hit
TTCACAGTGGCTAAGTTCTG116020.12604631642622952No Hit
CTCCCACTGCTTCACTTGACTAGCCT115790.12579644008785654No Hit
TATTGCACTTGTCCCGGCCTGTAAT115650.12564434144710776No Hit
TGAGGTAGTAGGTTGTATAGT114510.12440582394386777No Hit
ACTGGACTTGGAGTCAGAAGG112610.12234162810513449No Hit
AGCAGCATTGTACAGGGCTATGT112390.12210261595538643No Hit
GGAATACCGGGTGCTGTAGGCTTT110120.1196364451375314No Hit
AGCTACATTGTCTGCTGGGTTT108350.11771348375092196No Hit
AGCTCGGTCTGAGGCCCCTCAGT108120.11746360741254898No Hit
TATTGCACTTGTCCCGGCCTGTAAA107890.117213731074176No Hit
TTCACAGTGGCTAAGTTCCGC107590.11688780541542865No Hit
TGAGGTAGTAGGTTGTGTGGTT104830.11388928935495292No Hit
ATCCCGGACGAGCCCCCA104270.11328089479195784No Hit
AGCTACATCTGGCTACTGGGTCTC103830.11280287049246172No Hit
TGAGGTAGTAAGTTGTATTGTT102410.11126015570772421No Hit
ATCACATTGCCAGGGATTTCCA100880.10959793484811267No Hit
GGGAATACCGGGTGCTGTAGGCTT100340.10901126866236742No Hit
TCAGTGCACTACAGAACTTTGT100180.10883744164436882No Hit
TTCACAGTGGCTAAGTTCTGC98540.10705571470988325No Hit
TAGCAGCACGTAAATATTGGCGT98120.10659941878763694No Hit
AACATTCAACGCTGTCGGTGA97110.10550213573652084No Hit
TGTAAACATCCTACACTCAGCT96780.10514361751189873No Hit
ATCACATTGCCAGGGATTTCC96020.10431793917640542No Hit
AGCAGCATTGTACAGGGCTATG95800.10407892702665736No Hit
CGCGACCTCAGATCAGACGTGG94900.10310115005041527No Hit
GGAATACCGGGTGCTGTAGGCTT94430.1025905331850444No Hit
GGGAATACCGGGTGCTGTAGGCTTT92180.1001460907444392No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)