FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7675796
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG79358210.338758351576827No Hit
CAACGGAATCCCAAAAGCAGCTG5515457.185508838431871No Hit
TAGCAGCACGTAAATATTGGCG2914263.7966876660088413No Hit
AACATTCAACGCTGTCGGTGAGT2780063.621852378567643No Hit
TTCAAGTAATCCAGGATAGGCT2487743.241018911914803No Hit
CCCCCACTGCTAAATTTGACTG2355963.069336391952053No Hit
AAGCTGCCAGTTGAAGAACTGT2143972.79315656643298No Hit
AACATTCAACGCTGTCGGTGAG1962772.5570898444930013No Hit
TAGCTTATCAGACTGATGTTGACT1613042.1014628319981408No Hit
TAGCTTATCAGACTGATGTTGAC1571902.0478657848645274No Hit
TAGCTTATCAGACTGATGTTGA1368091.7823428345411994No Hit
TATTGCACTTGTCCCGGCCTGTAA1350171.758996721642941No Hit
TATTGCACTTGTCCCGGCCTGTAT1317311.7161868293529428No Hit
CATAAAGTAGAAAGCACTACT980151.2769359685953092No Hit
TGAGGTAGTAGGTTGTATAGTT952011.240275275684763No Hit
TGTAAACATCCTTGACTGGAAGCT791121.0306683502271297No Hit
TATTGCACTTGTCCCGGCCTGT770781.0041694698504233No Hit
TGAGGTAGTAGATTGTATAGTT734440.9568258458145579No Hit
TATTGCACTTGTCCCGGCCTGTA628360.818625195354332No Hit
TTCAAGTAATTCAGGATAGGTT579160.7545276086024172No Hit
AGCAGCATTGTACAGGGCTATGA433090.5642281269590802No Hit
TGTAAACATCCCCGACTGGAAGCT423110.5512262181016797No Hit
ACTGGACTTGGAGTCAGAAGGC343370.44734122689034467No Hit
TGAGGTAGTAGTTTGTACAGTT330930.4311344386953483No Hit
CCCATAAAGTAGAAAGCACTAC319770.41659522999308474No Hit
CATTGCACTTGTCTCGGTCTGA312450.4070587597690194No Hit
TTCTCACTACTGCACTTGACTAG264880.3450847312773815No Hit
CAACGGAATCCCAAAAGCAGCTGT259120.3375806235600842No Hit
CACCCGGCTGTGTGCACATGTGC258730.3370725329333922No Hit
TGTCAGTTTGTCAAATACCCCAA240340.313114105690146No Hit
CCCATAAAGTAGAAAGCACT239500.31201975664804016No Hit
CAACACCAGTCGATGGGCTGT212230.2764924966739606No Hit
CTCCCACTGCTTCACTTGACTAG209900.27345698087859555No Hit
AACATTCAACGCTGTCGGTGAGTT208330.27141159040704055No Hit
CCCCCACTGCTAAATTTGACTGGCTT204880.2669169425555343No Hit
TTCACAGTGGCTAAGTTCCG201500.2625134904575369No Hit
TGTAAACATCCTACACTCTCAGCT194830.25382383794462493No Hit
ACCGGGTGCTGTAGGCTTA193030.2514788042829695No Hit
ACCGGGTGCTGTAGGCTT178220.23218438843346018No Hit
CTTCTCACTACTGCACTTGACTAG177840.23168932577155515No Hit
TAGCTTATCAGACTGATGTTGACA177210.23086856398997577No Hit
TATTGCACTTGTCCCGGCCTGTAG169370.22065463959698772No Hit
TGTCAGTTTGTCAAATACCCCA165030.21500050287944078No Hit
CAAAGAATTCTCCTTTTGGGCT163680.2132417276331992No Hit
TATTGCACTTGTCCCGGCCTGTT161680.2106361346758043No Hit
TGAGGTAGTAGTTTGTGCTGTT160750.2094245339506157No Hit
TTATAAAGCAATGAGACTGATT157690.20543797672580147No Hit
CACTAGATTGTGAGCTCCTGGA156630.20405701245838218No Hit
TCGTACGACTCTTAGCGG153440.19990109169133727No Hit
CGCCTGTCTGAGCGTCGCT149590.19488532524835211No Hit
CACTAGATTGTGAGCTCCTGGAA149540.19482018542441723No Hit
TAGCAGCACGTAAATATTGGC149500.19476807356526932No Hit
ACCCCCCACTGCTAAATTTGACTG139480.18171405284872083No Hit
TTCACAGTGGCTAAGTTCCGC139420.18163588505999897No Hit
CCCCCCACTGCTAAATTTGACTGGCT136480.1778056634126285No Hit
AGAATTACTCTGAGACCT136030.1772194049972146No Hit
ACCGGGTGCTGTAGGCTTT133600.1740536095539798No Hit
AACGGAATCCCAAAAGCAGCTG128610.16755265512527953No Hit
TTCAAGTAATCCAGGATAGGCTT125950.1640872164919443No Hit
TGTAAACATCCCCGACTGGAAGC121270.1579901289716402No Hit
TTCAAGTAATCCAGGATAGGC116500.15177578976825334No Hit
CAAAGAATTCTCCTTTTGGGCTT116210.1513979787894311No Hit
TTCACAGTGGCTAAGTTCTG114390.14902688919820173No Hit
TCAGTGCACTACAGAACTTTGT111610.1454051149874228No Hit
CCCCCCACTGCTAAATTTGACTGG111570.14535300312827493No Hit
TATTGCACTTGTCCCGGCCTGTTT110640.1441414024030863No Hit
AGCTCGGTCTGAGGCCCCTCAGT106640.13893021648829645No Hit
AGCAGCATTGTACAGGGCTATGT100260.13061837495420672No Hit
CTACGCCTGTCTGAGCGTCGCT98360.12814306164468153No Hit
ACTGGACTTGGAGTCAGAAGGCA98070.12776525066585928No Hit
CTCCCACTGCTTCACTTGACTAGCCT97100.12650153808152273No Hit
TTCACAGTGGCTAAGTTCTGC96070.12515965770846438No Hit
ACTGGACTTGGAGTCAGAAGG95700.12467762301134633No Hit
ACTGGACTTGGAGTCAGAAGGCT95480.12439100778603289No Hit
ATCCCGGACGAGCCCCCA89010.11596191456886035No Hit
CCCATAAAGTAGAAAGCACTA87390.11385138427337047No Hit
CAAAGTGCTGTTCGTGCAGGT86740.11300456656221713No Hit
TAGCAGCACGTAAATATTGGCGT84470.11004721855557391No Hit
TGTAAACATCCTACACTCAGCT84110.10957821182324282No Hit
TATTGCACTTGTCCCGGCCTGTAAT83640.10896589747825504No Hit
CAACGGAATCCCAAAAGCAGCT83040.10818421959103655No Hit
CAAAGTGCTGTTCGTGCAGGTAG81850.10663389178138657No Hit
TATTGCACTTGTCCCGGCCTGTAAA81450.10611277318990761No Hit
TGAGGTAGTAGGTTGTATAGT78780.10263430659178539No Hit
TGTCAGTTTGTCAAATACC78100.10174840498627113No Hit
CGCGACCTCAGATCAGACGTGGCG77720.1012533423243661No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)