FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9289144
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG107880611.61362123356038No Hit
CAACGGAATCCCAAAAGCAGCTG8823139.498324065166823No Hit
CCCCCACTGCTAAATTTGACTG3601423.877020315327225No Hit
TAGCAGCACGTAAATATTGGCG3139093.379310300281705No Hit
TTCAAGTAATCCAGGATAGGCT2816283.031797117150945No Hit
AAGCTGCCAGTTGAAGAACTGT2768512.9803714960172862No Hit
TAGCTTATCAGACTGATGTTGAC2041292.1975006523744276No Hit
AACATTCAACGCTGTCGGTGAGT2000452.153535352665434No Hit
AACATTCAACGCTGTCGGTGAG1525931.6427024922856186No Hit
TAGCTTATCAGACTGATGTTGACT1324291.4256318989133983No Hit
TATTGCACTTGTCCCGGCCTGTAA1204061.296201243085477No Hit
TATTGCACTTGTCCCGGCCTGTAT1176841.2668982201158685No Hit
TGAGGTAGTAGGTTGTATAGTT926730.9976484377893162No Hit
TAGCTTATCAGACTGATGTTGA926120.9969917572598723No Hit
CAACGGAATCCCAAAAGCAGCT790300.8507780695400997No Hit
TGTAAACATCCTTGACTGGAAGCT760040.8182024091778533No Hit
TATTGCACTTGTCCCGGCCTGT754280.8120016225391704No Hit
TGAGGTAGTAGATTGTATAGTT676040.7277742706970631No Hit
TATTGCACTTGTCCCGGCCTGTA663670.7144576507803088No Hit
CATAAAGTAGAAAGCACTACT644020.6933039255285525No Hit
AGCAGCATTGTACAGGGCTATGA583460.6281095437857352No Hit
CAACGGAATCCCAAAAGCAGCTGT471200.507258795858908No Hit
TTCAAGTAATTCAGGATAGGTT415720.4475331634432623No Hit
CATTGCACTTGTCTCGGTCTGA377600.4064960129803134No Hit
TGTAAACATCCCCGACTGGAAGCT358950.38641881318666177No Hit
ACTGGACTTGGAGTCAGAAGGC344060.3703893491154836No Hit
ACCGGGTGCTGTAGGCTT288710.3108036650093916No Hit
CCCATAAAGTAGAAAGCACTAC279660.3010611096135446No Hit
TTCTCACTACTGCACTTGACTAG276790.2979714815487843No Hit
TGAGGTAGTAGTTTGTACAGTT267670.2881535693708699No Hit
TAGCTTATCAGACTGATGTTGACA262230.28229727087878065No Hit
ACCGGGTGCTGTAGGCTTA251150.2703693688029812No Hit
CCCATAAAGTAGAAAGCACT250570.2697449840372805No Hit
CTTCTCACTACTGCACTTGACTAG245080.263834859272286No Hit
TGTAAACATCCTACACTCTCAGCT238600.2568589743037679No Hit
CACCCGGCTGTGTGCACATGTGC225250.24248735943807093No Hit
CCCCCACTGCTAAATTTGACTGGCTT225110.2423366458739363No Hit
CAACACCAGTCGATGGGCTGT215590.23208812351278008No Hit
CAAAGAATTCTCCTTTTGGGCT214350.23075323194473035No Hit
ACCGGGTGCTGTAGGCTTT206870.2227008215181076No Hit
TTCACAGTGGCTAAGTTCTGC206650.22246398591732455No Hit
TTCACAGTGGCTAAGTTCCGC190810.205411822660947No Hit
TAGCAGCACGTAAATATTGGC184700.19883425211192762No Hit
ACCCCCCACTGCTAAATTTGACTG181630.1955293189555464No Hit
CAACGGAATCCCAAAAGCAGCTGA181140.19500182148107512No Hit
TCGTTTCCCGGCCAATGCACCA175620.18905940095233748No Hit
TGAGGTAGTAGTTTGTGCTGTT173270.18652956612579158No Hit
AACGGAATCCCAAAAGCAGCTG172380.18557145846807843No Hit
CTCCCACTGCTTCACTTGACTAG172110.18528079659439017No Hit
CCCCCCACTGCTAAATTTGACTGG164570.17716379464028117No Hit
GCCTGTCTGAGCGTCGCT161660.17403110555719664No Hit
TACGCCTGTCTGAGCGTCGCT153470.16521436205531964No Hit
TAGCAGCACATCATGGTTTACA151790.16340579928570384No Hit
CTACGCCTGTCTGAGCGTCGCT148240.15958413390943232No Hit
TATTGCACTTGTCCCGGCCTGTAG147370.1586475567608813No Hit
TGATGTAGTAGGTTGTATAGTT145300.15641914906260468No Hit
TATTGCACTTGTCCCGGCCTGTT133770.14400681053065814No Hit
TTCAAGTAATCCAGGATAGGCTT128720.1385703569672297No Hit
CAAAGAATTCTCCTTTTGGGCTT128080.13788138067404274No Hit
TGTCAGTTTGTCAAATACCCCA127180.13691250776174854No Hit
AACATTCAACGCTGTCGGTGAGTT122370.13173442030826524No Hit
ATCACATTGCCAGGGATTTCC122120.13146528894373907No Hit
AGCAGCATTGTACAGGGCTATGT121500.13079784315971418No Hit
CACTAGATTGTGAGCTCCTGGA120400.12961366515579908No Hit
CGCGACCTCAGATCAGACGTGG120270.12947371684624548No Hit
TGTCAGTTTGTCAAATACCCCAA120110.12930147277294873No Hit
CCCCCCACTGCTAAATTTGACTGGCT119460.1286017312251807No Hit
TTCACAGTGGCTAAGTTCCG119350.1284833134247892No Hit
TTCAAGTAATCCAGGATAGGC115820.1246831785576798No Hit
CGCCTGTCTGAGCGTCGCT114260.12300379884303655No Hit
TCAGTGCACTACAGAACTTTGT112680.12130288861923122No Hit
AATGCACCCGGGCAAGGATTCT110190.11862234022855067No Hit
TGTAAACATCCCCGACTGGAAGC109890.11829938259111927No Hit
CTCCCACTGCTTCACTTGACTAGCCT109790.1181917300453088No Hit
TGTAAACATCCTACACTCAGCT107980.1162432189661394No Hit
CGCCCCACTGCTAAATTTGACTG107090.11528511130842628No Hit
TAGCAGCACGTAAATATTGGCGT106330.11446695196026674No Hit
TGATGTAGTAGATTGTATAGTT105750.11384256719456604No Hit
CACTAGATTGTGAGCTCCTGGAA105160.11320741717428431No Hit
ACTGGACTTGGAGTCAGAAGGCA102290.11011778910952398No Hit
TCGTACGACTCTTAGCGG100780.10849223566778597No Hit
TATTGCACTTGTCCCGGCCTGA100530.1082231043032598No Hit
CGTTCGCGCTTTCCCCTG100250.10792167717499052No Hit
CAAAGTGCTGTTCGTGCAGGTAG99630.10725423139096563No Hit
TATTGCACTTGTCCCGGCCTGTTT99380.10698510002643946No Hit
CGCGACCTCAGATCAGACGTGGCG98760.10631765424241459No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)