FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13310885
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG138433210.399999699494062No Hit
CAACGGAATCCCAAAAGCAGCTG12247809.201341608766059No Hit
CCCCCACTGCTAAATTTGACTG4747353.5665171774829396No Hit
TAGCAGCACGTAAATATTGGCG4718073.544520142725296No Hit
TTCAAGTAATCCAGGATAGGCT4187653.1460342419005203No Hit
AAGCTGCCAGTTGAAGAACTGT3683932.7676071125248245No Hit
AACATTCAACGCTGTCGGTGAGT3132982.3536977443648563No Hit
AACATTCAACGCTGTCGGTGAG2344841.7615958668413108No Hit
TAGCTTATCAGACTGATGTTGAC1769661.3294833514075135No Hit
TATTGCACTTGTCCCGGCCTGTAT1423001.069049879102704No Hit
TATTGCACTTGTCCCGGCCTGTAA1416321.0640314299161926No Hit
TATTGCACTTGTCCCGGCCTGT1263850.9494860784989126No Hit
TGAGGTAGTAGGTTGTATAGTT1235920.9285032512864472No Hit
TAGCTTATCAGACTGATGTTGACT1198790.9006087874698039No Hit
TAGCTTATCAGACTGATGTTGA1147980.8624370205286875No Hit
CATAAAGTAGAAAGCACTACT962630.7231900808999552No Hit
TGTAAACATCCTTGACTGGAAGCT874500.6569811098210224No Hit
ACCGGGTGCTGTAGGCTT861960.6475602486235889No Hit
TATTGCACTTGTCCCGGCCTGTA851410.6396344044742329No Hit
CAACGGAATCCCAAAAGCAGCT835000.627306148313955No Hit
TGAGGTAGTAGATTGTATAGTT806480.6058800748410041No Hit
AGCAGCATTGTACAGGGCTATGA700170.5260131088203377No Hit
ACCGGGTGCTGTAGGCTTA668270.5020477601601997No Hit
TTCAAGTAATTCAGGATAGGTT664870.49949345967604714No Hit
CAACGGAATCCCAAAAGCAGCTGT638230.4794797641178629No Hit
ACCGGGTGCTGTAGGCTTT613440.4608559085289971No Hit
CATTGCACTTGTCTCGGTCTGA513730.38594729050697985No Hit
ACTGGACTTGGAGTCAGAAGGC502340.3773903838850685No Hit
TTCTCACTACTGCACTTGACTAG500350.37589536683699093No Hit
GGAATACCGGGTGCTGTAGGCTTT478640.3595854069808281No Hit
TGTAAACATCCCCGACTGGAAGCT473560.3557689815515647No Hit
GGAATACCGGGTGCTGTAGGCTT406170.30514124342596305No Hit
CCCATAAAGTAGAAAGCACTAC387460.29108507811464074No Hit
CCCATAAAGTAGAAAGCACT346030.2599601754503927No Hit
TGAGGTAGTAGTTTGTACAGTT331260.24886399364129433No Hit
CTCCCACTGCTTCACTTGACTAG324170.24353752586698782No Hit
CTTCTCACTACTGCACTTGACTAG322320.24214768589766947No Hit
TTAATGCTAATCGTGATAGGGGTT304330.22863243127710892No Hit
CACCCGGCTGTGTGCACATGTGC296950.22308809669680116No Hit
CCCCCACTGCTAAATTTGACTGGCTT296410.22268241367872985No Hit
TGTAAACATCCTACACTCTCAGCT283020.21262297736025815No Hit
TAGCAGCACGTAAATATTGGC280530.2107523278880405No Hit
CAACACCAGTCGATGGGCTGT270360.20311196438103102No Hit
AACGGAATCCCAAAAGCAGCTG266250.20002426585459943No Hit
TGAGGTAGTAGTTTGTGCTGTT259330.19482551310450055No Hit
TGAGAACTGAATTCCATAGGCTGT255300.19179791576593141No Hit
CAAAGAATTCTCCTTTTGGGCT245820.18467592500423527No Hit
CAACGGAATCCCAAAAGCAGCTGA245470.1844129823073372No Hit
TGAGAACTGAATTCCATGGGTT237220.17821504730902565No Hit
CTACGCCTGTCTGAGCGTCGCT235280.1767575935033621No Hit
TACGCCTGTCTGAGCGTCGCT234420.17611150573384113No Hit
CGCCTGTCTGAGCGTCGCT226550.17019905137787608No Hit
GCCTGTCTGAGCGTCGCT226190.16992859603249522No Hit
TTCACAGTGGCTAAGTTCCGC222810.1673893208453082No Hit
CCCCCCACTGCTAAATTTGACTGG222390.16707378960903052No Hit
TTCACAGTGGCTAAGTTCTGC220340.16553369667005613No Hit
TAGCTTATCAGACTGATGTTGACA217580.16346020568880282No Hit
TATTGCACTTGTCCCGGCCTGTT216030.16229574517396853No Hit
TGAATACCGGGTGCTGTAGGCTTT214280.1609810316894782No Hit
ACCCCCCACTGCTAAATTTGACTG213850.1606579878047177No Hit
ATCCCGGACGAGCCCCCA207130.15560948802427488No Hit
CGCGACCTCAGATCAGACGTGG202840.15238656182515287No Hit
TTCACAGTGGCTAAGTTCCG194720.14628629125711776No Hit
TCGTACGACTCTTAGCGG189560.14240976463999203No Hit
TGAATACCGGGTGCTGTAGGCTT184950.13894643368942033No Hit
CACTAGATTGTGAGCTCCTGGA181740.13653487352644098No Hit
TATTGCACTTGTCCCGGCCTGTAG180390.13552066598126272No Hit
AACATTCAACGCTGTCGGTGAGTT179470.13482950232084492No Hit
TTCAAGTAATCCAGGATAGGC175370.13174931644289617No Hit
CCCCCCACTGCTAAATTTGACTGGCT173890.1306374444674415No Hit
AATGCACCCGGGCAAGGATTCT171220.12863156732253342No Hit
TCGTTTCCCGGCCAATGCACCA167230.12563402057789547No Hit
AGCTCGGTCTGAGGCCCCTCAGT165390.12425169325705993No Hit
TTCAAGTAATCCAGGATAGGCTT163750.12301961890588042No Hit
TGTCAGTTTGTCAAATACCCCAA163270.12265901177870592No Hit
TGTAAACATCCCCGACTGGAAGC163250.12264398648174032No Hit
TATTGCACTTGTCCCGGCCTGA157850.11858715630102731No Hit
CAAAGAATTCTCCTTTTGGGCTT157190.1180913215011624No Hit
TAGCAGCACATCATGGTTTACA153560.11536423010190533No Hit
TGTCAGTTTGTCAAATACCCCA153250.11513133799893845No Hit
CGCGACCTCAGATCAGACGTGGCG152740.11474819292631556No Hit
AGCAGCATTGTACAGGGCTATGT148370.11146516553933115No Hit
CTCCCACTGCTTCACTTGACTAGCCT144190.10832487847351997No Hit
TCAGTGCACTACAGAACTTTGT142890.10734823417075574No Hit
TGAGGTAGTAGGTTGTGTGGTT140770.10575555269240174No Hit
AATGACACGATCACTCCCGTTG140140.10528225583798523No Hit
TAGCAGCACGTAAATATTGGCGT138590.10411779532315095No Hit
ACTGGACTTGGAGTCAGAAGGCT135330.10166867191775752No Hit
ACTGGACTTGGAGTCAGAAGGCA134830.10129303949361745No Hit
TGTAAACATCCTACACTCAGCT134820.10128552684513464No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)