FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9071268
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG138371115.253777090479522No Hit
CAACGGAATCCCAAAAGCAGCTG8931269.845657740461423No Hit
CCCCCACTGCTAAATTTGACTG4846255.342417399640271No Hit
TAGCAGCACGTAAATATTGGCG2633392.9030009917025934No Hit
AAGCTGCCAGTTGAAGAACTGT2227782.45586394316649No Hit
AACATTCAACGCTGTCGGTGAGT2202732.428249281136882No Hit
TTCAAGTAATCCAGGATAGGCT2097042.311738557388008No Hit
AACATTCAACGCTGTCGGTGAG1714501.8900334550803704No Hit
TAGCTTATCAGACTGATGTTGAC1694671.8681732256174108No Hit
TAGCTTATCAGACTGATGTTGACT1347821.485812126816229No Hit
TATTGCACTTGTCCCGGCCTGTAA877710.9675714574853262No Hit
TAGCTTATCAGACTGATGTTGA870540.9596673805690671No Hit
TGAGGTAGTAGGTTGTATAGTT792710.8738690114766755No Hit
TGTAAACATCCTTGACTGGAAGCT762070.8400920356448514No Hit
TATTGCACTTGTCCCGGCCTGTAT733440.8085308470657023No Hit
TATTGCACTTGTCCCGGCCTGT636960.7021730589372952No Hit
AGCAGCATTGTACAGGGCTATGA599140.6604809823720345No Hit
CAACGGAATCCCAAAAGCAGCTGT478780.5277983188237851No Hit
TGTAAACATCCCCGACTGGAAGCT477740.5266518418373264No Hit
CATAAAGTAGAAAGCACTACT457230.5040419928062979No Hit
ACTGGACTTGGAGTCAGAAGGC443590.4890055061762038No Hit
TATTGCACTTGTCCCGGCCTGTA441010.48616136134441185No Hit
TGAGGTAGTAGATTGTATAGTT389040.4288705834730051No Hit
CAACGGAATCCCAAAAGCAGCT381190.42021688698867676No Hit
TTCAAGTAATTCAGGATAGGTT358360.39504951237247093No Hit
CCCCCACTGCTAAATTTGACTGGCTT319410.352111744466154No Hit
ACCGGGTGCTGTAGGCTT306660.3380563775648564No Hit
CATTGCACTTGTCTCGGTCTGA292420.32235846190411305No Hit
CCCATAAAGTAGAAAGCACTAC278960.30752040398321384No Hit
TAGCTTATCAGACTGATGTTGACA244040.26902523439942466No Hit
CGCCTGTCTGAGCGTCGCT238280.2626755157051914No Hit
TTAATGCTAATCGTGATAGGGGTT227780.25110050766882863No Hit
CACCCGGCTGTGTGCACATGTGC225530.24862014880389377No Hit
ACCCCCCACTGCTAAATTTGACTG224200.24715398111928785No Hit
ACCGGGTGCTGTAGGCTTT221560.24424369338443092No Hit
CAAAGAATTCTCCTTTTGGGCT220180.24272240661393757No Hit
TGAGAACTGAATTCCATAGGCTGT209460.23090487459967007No Hit
CCCCCCACTGCTAAATTTGACTGG204540.22548115654834586No Hit
TGTCAGTTTGTCAAATACCCCAA202110.22280236897421618No Hit
CAACACCAGTCGATGGGCTGT198510.21883379479032036No Hit
CGCGACCTCAGATCAGACGTGG190920.21046671755260674No Hit
CCCATAAAGTAGAAAGCACT190370.2098604076078449No Hit
TAGCAGCACGTAAATATTGGC189980.20943047873792286No Hit
ACCGGGTGCTGTAGGCTTA179060.19739247038010563No Hit
AACGGAATCCCAAAAGCAGCTG174090.19191363324289393No Hit
CGCGACCTCAGATCAGACGTGGCG169670.1870411060504441No Hit
TGAGGTAGTAGTTTGTACAGTT168220.1854426525597083No Hit
TTCTCACTACTGCACTTGACTAG166260.1832819843929206No Hit
AACATTCAACGCTGTCGGTGAGTT163450.1801842917660464No Hit
TGTAAACATCCTACACTCTCAGCT161820.1783874095661158No Hit
CAAAGAATTCTCCTTTTGGGCTT160630.1770755753219947No Hit
TGAGGTAGTAGTTTGTGCTGTT150500.16590844852119901No Hit
TCGTTTCCCGGCCAATGCACCA149630.16494937642675753No Hit
CCCCCCACTGCTAAATTTGACTGGCT147800.16293201788327719No Hit
CTTCTCACTACTGCACTTGACTAG140760.1551712505903254No Hit
ACTGGACTTGGAGTCAGAAGGCA136950.15097117624570236No Hit
TGTAAACATCCCCGACTGGAAGC133920.1476309596409234No Hit
CACTAGATTGTGAGCTCCTGGA133390.14704669733051653No Hit
TGTCAGTTTGTCAAATACCCCA130640.14401514760670725No Hit
TCGTACGACTCTTAGCGG129590.14285764680307098No Hit
ACTGGACTTGGAGTCAGAAGGCT123830.13650792810883772No Hit
CACTAGATTGTGAGCTCCTGGAA120940.13332204494454358No Hit
TTCAAGTAATCCAGGATAGGCTT119590.13183382962558265No Hit
TATTGCACTTGTCCCGGCCTGTT114080.1257597063607866No Hit
CTACGCCTGTCTGAGCGTCGCT113740.125384896576752No Hit
TGAGAACTGAATTCCATGGGTT112070.12354391910811145No Hit
AGCAGCATTGTACAGGGCTATGT110890.12224310868116785No Hit
TATTGCACTTGTCCCGGCCTGTAG108120.11918951132300357No Hit
CCCCCCACTGCTAAATTTGACTGT105140.11590441380411205No Hit
CTCCCACTGCTTCACTTGACTAG102890.1134240549391772No Hit
CTCCCACTGCTTCACTTGACTAGCCT99790.11000667161415582No Hit
TGATGTAGTAGGTTGTATAGTT99690.10989643344238094No Hit
TTCACAGTGGCTAAGTTCCGC98730.10883814699334206No Hit
TAGCAGCACGTAAATATTGGCGT97780.10779088436148067No Hit
ACCACAGGGTAGAACCACGGAC96450.10632471667687472No Hit
CAAAGTGCTGTTCGTGCAGGTAG95430.10520028732477092No Hit
AGCAGCATTGTACAGGGCTATG92550.10202542797765428No Hit
TTCACAGTGGCTAAGTTCTGC92190.1016285705592647No Hit
TTATAAAGCAATGAGACTGATT91360.10071359373353317No Hit
TACGCCTGTCTGAGCGTCGCT91320.10066949846482322No Hit
ACTGGACTTGGAGTCAGAAGG91200.10053721265869336No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)