FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8991591
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG8755289.737186666964723No Hit
CAACGGAATCCCAAAAGCAGCTG5274125.865613771800786No Hit
CCCCCACTGCTAAATTTGACTG3495383.887387671436568No Hit
TTCAAGTAATCCAGGATAGGCT2994553.3303894716741453No Hit
TAGCAGCACGTAAATATTGGCG2666042.9650369995699313No Hit
AAGCTGCCAGTTGAAGAACTGT2547292.832969159740473No Hit
AACATTCAACGCTGTCGGTGAGT2188472.4339074141606307No Hit
AACATTCAACGCTGTCGGTGAG1595721.7746803652434813No Hit
TAGCTTATCAGACTGATGTTGAC1508941.6781679682716883No Hit
TAGCTTATCAGACTGATGTTGACT1381121.5360129258548347No Hit
TAGCTTATCAGACTGATGTTGA1336621.48652224061348No Hit
TGAGGTAGTAGGTTGTATAGTT1227801.3654980525693396No Hit
CATAAAGTAGAAAGCACTACT1173521.3051305380771878No Hit
TATTGCACTTGTCCCGGCCTGTAA977691.0873381585083217No Hit
TATTGCACTTGTCCCGGCCTGTAT947231.0534620624981719No Hit
TGAGGTAGTAGATTGTATAGTT826440.9191254361992222No Hit
TGTAAACATCCTTGACTGGAAGCT813530.9047675767280785No Hit
TATTGCACTTGTCCCGGCCTGT778780.8661203562306159No Hit
ACCGGGTGCTGTAGGCTT719480.8001698475831475No Hit
ACCGGGTGCTGTAGGCTTA640760.7126213814663056No Hit
TTCAAGTAATTCAGGATAGGTT635700.706993901301783No Hit
TATTGCACTTGTCCCGGCCTGTA598220.6653105106760305No Hit
ACCGGGTGCTGTAGGCTTT538060.5984035528306392No Hit
AGCAGCATTGTACAGGGCTATGA494790.5502808123723599No Hit
TTAATGCTAATCGTGATAGGGGTT433950.48261759237047147No Hit
TGTAAACATCCCCGACTGGAAGCT403970.4492753284707901No Hit
CCCATAAAGTAGAAAGCACTAC364160.4050006278088049No Hit
TTCTCACTACTGCACTTGACTAG351110.3904870673054413No Hit
ACTGGACTTGGAGTCAGAAGGC343970.38254631466222166No Hit
CATTGCACTTGTCTCGGTCTGA343600.3821348190770688No Hit
TGAGGTAGTAGTTTGTACAGTT336790.37456107600979627No Hit
GCCTGTCTGAGCGTCGCT328940.3658306967031752No Hit
CAACGGAATCCCAAAAGCAGCTGT300090.3337451625635552No Hit
TCGTACGACTCTTAGCGG296860.3301529173201939No Hit
CAACACCAGTCGATGGGCTGT296440.32968581422353394No Hit
CCCATAAAGTAGAAAGCACT272620.3031943957415323No Hit
TTCACAGTGGCTAAGTTCCG272040.30254934860804944No Hit
CAACGGAATCCCAAAAGCAGCT249780.27779288448507056No Hit
CAAAGAATTCTCCTTTTGGGCT234040.26028763986262277No Hit
CTTCTCACTACTGCACTTGACTAG224840.2500558577453089No Hit
CGCCTGTCTGAGCGTCGCT222020.24691959409630623No Hit
TGAGGTAGTAGTTTGTGCTGTT207940.23126051885589546No Hit
ATCCCGGACGAGCCCCCA205300.22832444224831847No Hit
TGTAAACATCCTACACTCTCAGCT191290.21274321752401773No Hit
CACCCGGCTGTGTGCACATGTGC179030.1991082557024669No Hit
CCCCCACTGCTAAATTTGACTGGCTT175790.1955048889568042No Hit
TTATAAAGCAATGAGACTGATT173600.19306927995279144No Hit
TGATGTAGTAGGTTGTATAGTT171430.19065591395338155No Hit
ACCCCCCACTGCTAAATTTGACTG168310.187186005235336No Hit
TTCACAGTGGCTAAGTTCTG168030.186874603170896No Hit
TAGCTTATCAGACTGATGTTGACA164540.1829931988676976No Hit
AACATTCAACGCTGTCGGTGAGTT164160.18257058178024332No Hit
CTCCCACTGCTTCACTTGACTAG162500.18072441239820627No Hit
TAGCAGCACGTAAATATTGGC156150.17366225843679944No Hit
TGAGAACTGAATTCCATAGGCTGT152960.1701144992026439No Hit
TATTGCACTTGTCCCGGCCTGTT145130.1614063629006257No Hit
CAAAGAATTCTCCTTTTGGGCTT144610.16082804478095145No Hit
TTCAAGTAATCCAGGATAGGCTT142650.15864823032987155No Hit
TGTCAGTTTGTCAAATACCCCAA141970.15789196817337445No Hit
CCCCCCACTGCTAAATTTGACTGG141610.15749159409052305No Hit
TGAGAACTGAATTCCATGGGTT140140.1558567332522131No Hit
TTCAAGTAATCCAGGATAGGC138760.154321965934616No Hit
CGCGACCTCAGATCAGACGTGG135130.15028486059919763No Hit
TGTCAGTTTGTCAAATACCCCA127210.1414766307764666No Hit
CACTAGATTGTGAGCTCCTGGA124900.1389075637448367No Hit
TATTGCACTTGTCCCGGCCTGTAG124030.13793999304461246No Hit
AACGGAATCCCAAAAGCAGCTG122560.13630513220630253No Hit
TTCACAGTGGCTAAGTTCTGC122370.1360938236625754No Hit
TCCTGTCTGAGCGTCGCT120800.1343477478012512No Hit
TCAGTGCACTACAGAACTTTGT119310.1326906439583384No Hit
TGATGTAGTAGATTGTATAGTT117400.13056643701876564No Hit
TGAGGTAGTAGGTTGTATAGT117010.13013269842900996No Hit
CGCGACCTCAGATCAGACGTGGCG114610.12746353787666723No Hit
TGTAAACATCCCCGACTGGAAGC113050.12572858351764443No Hit
AGCAGCATTGTACAGGGCTATGT111120.12358213357346881No Hit
ACTGGACTTGGAGTCAGAAGG108800.1210019450395375No Hit
TTCACAGTGGCTAAGTTCCGC107740.11982306579558612No Hit
CCCATAAAGTAGAAAGCACTA107720.11980082279098327No Hit
TCGTTTCCCGGCCAATGCACCA104820.11657558712356914No Hit
CAAAGTGCTGTTCGTGCAGGT103190.11476278224843633No Hit
CACTAGATTGTGAGCTCCTGGAA102560.11406212760344638No Hit
CCCCCCACTGCTAAATTTGACTGGCT100510.11178221963165362No Hit
AGCAGCATTGTACAGGGCTATG100330.11158203259022792No Hit
ACCTGGTGCTGTAGGCTT100130.11135960254419934No Hit
CTACGCCTGTCTGAGCGTCGCT99920.11112605099586935No Hit
ACTGGACTTGGAGTCAGAAGGCA97520.10845689044352662No Hit
ACTGGACTTGGAGTCAGAAGGCT94280.10485352369786394No Hit
TAGCAGCACGTAAATATTGGCGT92250.10259585873067403No Hit
GGAATACCGGGTGCTGTAGGCTT91390.1016394095327512No Hit
GGAATACCGGGTGCTGTAGGCTTT90600.10076081085093838No Hit
ACCACAGGGTAGAACCACGGAC90170.100282586251977No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)