FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7944970
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG117115514.740836025812559No Hit
CAACGGAATCCCAAAAGCAGCTG6517708.203555205368932No Hit
CCCCCACTGCTAAATTTGACTG3692134.6471289381835295No Hit
TAGCAGCACGTAAATATTGGCG2726583.4318317123916136No Hit
TTCAAGTAATCCAGGATAGGCT2048962.578939882718248No Hit
AACATTCAACGCTGTCGGTGAGT1876492.361859138549296No Hit
AAGCTGCCAGTTGAAGAACTGT1873282.3578188463896024No Hit
AACATTCAACGCTGTCGGTGAG1401801.764386775532192No Hit
TATTGCACTTGTCCCGGCCTGTAA1151961.4499236623926837No Hit
TATTGCACTTGTCCCGGCCTGTAT1134131.4274817903654766No Hit
TAGCTTATCAGACTGATGTTGAC1109861.396934160859009No Hit
TAGCTTATCAGACTGATGTTGACT1102421.3875697453860745No Hit
TGAGGTAGTAGGTTGTATAGTT726570.9145031384637072No Hit
TGTAAACATCCTTGACTGGAAGCT719580.9057051190879261No Hit
TAGCTTATCAGACTGATGTTGA639950.8054781830516666No Hit
TATTGCACTTGTCCCGGCCTGT569570.7168938334568916No Hit
AGCAGCATTGTACAGGGCTATGA522900.6581522648921266No Hit
TATTGCACTTGTCCCGGCCTGTA493380.6209966809188706No Hit
TGAGGTAGTAGATTGTATAGTT488230.6145145922514497No Hit
CATAAAGTAGAAAGCACTACT480800.605162763358452No Hit
TGTAAACATCCCCGACTGGAAGCT423170.5326263031830202No Hit
ACTGGACTTGGAGTCAGAAGGC409120.5149421583719007No Hit
ACCGGGTGCTGTAGGCTTA370710.466597104834883No Hit
CAACGGAATCCCAAAAGCAGCTGT370230.46599294899791943No Hit
CCCCCACTGCTAAATTTGACTGGCTT330080.41545783055190894No Hit
ACCGGGTGCTGTAGGCTT310030.3902217377787455No Hit
TTCAAGTAATTCAGGATAGGTT294530.3707125388767988No Hit
ACCCCCCACTGCTAAATTTGACTG282690.3558100282316988No Hit
CCCATAAAGTAGAAAGCACTAC258420.32526239872523116No Hit
ACCGGGTGCTGTAGGCTTT250300.3150420958165984No Hit
TTCTCACTACTGCACTTGACTAG247720.3117947581929196No Hit
CAAAGAATTCTCCTTTTGGGCT240480.30268207431871985No Hit
CATTGCACTTGTCTCGGTCTGA233950.2944630376200288No Hit
TGTAAACATCCTACACTCTCAGCT230880.29059895757944965No Hit
CTTCTCACTACTGCACTTGACTAG224770.2829085572381016No Hit
GGAATACCGGGTGCTGTAGGCTTT220900.27803755080258324No Hit
CCCATAAAGTAGAAAGCACT211200.26582856826394563No Hit
CCCCCCACTGCTAAATTTGACTGGCT204960.25797454238341994No Hit
TGAGGTAGTAGTTTGTACAGTT196240.2469990446785828No Hit
CCCCCCACTGCTAAATTTGACTGG194480.24478380660971658No Hit
CTCCCACTGCTTCACTTGACTAG190970.2403659170519209No Hit
CGCGACCTCAGATCAGACGTGGCG190420.2396736551554002No Hit
GGAATACCGGGTGCTGTAGGCTT168940.212637681451283No Hit
TAGCTTATCAGACTGATGTTGACA167530.21086297368020268No Hit
AACGGAATCCCAAAAGCAGCTG166050.20900015984956521No Hit
GACCCGCGGGCGCTCTCCAGTCCT163400.20566471616632911No Hit
CACTAGATTGTGAGCTCCTGGAA162590.2046452031914532No Hit
TATTGCACTTGTCCCGGCCTGTAG161490.20326067939841183No Hit
CGCGACCTCAGATCAGACGTGG157380.19808759504441176No Hit
CACCCGGCTGTGTGCACATGTGC153380.1930529630697158No Hit
TAGCAGCACGTAAATATTGGC151810.19107687001964765No Hit
TGTCAGTTTGTCAAATACCCCAA147110.1851611774493799No Hit
CACTAGATTGTGAGCTCCTGGA146490.18438080949330207No Hit
AGCTCGGTCTGAGGCCCCTCAGT145990.18375148049646506No Hit
TCGTACGACTCTTAGCGG140470.17680368837138466No Hit
AACATTCAACGCTGTCGGTGAGTT138800.1747017295219491No Hit
CAAAGAATTCTCCTTTTGGGCTT130090.16373881839704868No Hit
TGAGGTAGTAGTTTGTGCTGTT129830.16341156731869347No Hit
TCAGTGCACTACAGAACTTTGT127670.16069286605235764No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC123620.155595301177978No Hit
TATTGCACTTGTCCCGGCCTGTT122190.15379542024702422No Hit
CAACACCAGTCGATGGGCTGT121280.15265004147278088No Hit
TGTCAGTTTGTCAAATACCCCA119650.1505984289430923No Hit
TGTAAACATCCCCGACTGGAAGC118580.14925166488986114No Hit
CCCCCCACTGCTAAATTTGACTGT115200.14499740087124308No Hit
TTCAAGTAATCCAGGATAGGCTT115100.14487153507187567No Hit
ACTGGACTTGGAGTCAGAAGGCA112470.1415612645485131No Hit
CAACGGAATCCCAAAAGCAGCT108030.13597282305660058No Hit
CCCCCCACTGCTAAATTTGACTGA107550.13536866721963708No Hit
CTCCCACTGCTTCACTTGACTAGCCT106080.13351843996893634No Hit
ACTGGACTTGGAGTCAGAAGGCT105860.13324153521032805No Hit
TTCAAGTAATCCAGGATAGGC103630.13043472788443505No Hit
TATTGCACTTGTCCCGGCCTGTTT100510.12650771494417223No Hit
TATTGCACTTGTCCCGGCCTGTAAT100280.1262182236056272No Hit
AGCAGCATTGTACAGGGCTATGT99430.12514836431100432No Hit
GGGAATACCGGGTGCTGTAGGCTT96740.1217625743080213No Hit
TCGTTTCCCGGGCGGCGCACCA96340.1212591111105517No Hit
TATTGCACTTGTCCCGGCCTGTAAA95580.12030253103535947No Hit
TTCACAGTGGCTAAGTTCCG94480.11891800724231809No Hit
ACTGGACTTGGAGTCAGAAGG92270.11613637307629858No Hit
CGCCTGTCTGAGCGTCGCT91360.11499099430205527No Hit
GGGAATACCGGGTGCTGTAGGCTTT90660.11410993370648348No Hit
GTCAGGATGGCCGAGCGGTCTAAGG90180.11350577786951996No Hit
ATCCCGGACGAGCCCCCA83940.1056517519889943No Hit
TCAGTGCACTACAGAACTTTGTC83850.10553847276956363No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)