FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7063325
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG79187111.211023137120266No Hit
CCCCCCACTGCTAAATTTGACTG6816839.651021296627297No Hit
AACATTCAACGCTGTCGGTGAGT3398454.811402561824636No Hit
AAGCTGCCAGTTGAAGAACTGT2539313.595063231551712No Hit
TAGCAGCACGTAAATATTGGCG2504253.545426551942605No Hit
TTCAAGTAATCCAGGATAGGCT2273023.218059483316993No Hit
CCCCCACTGCTAAATTTGACTG2088002.9561148609189014No Hit
AACATTCAACGCTGTCGGTGAG2070642.931537200964135No Hit
TATTGCACTTGTCCCGGCCTGTAA1090341.5436639259838674No Hit
TATTGCACTTGTCCCGGCCTGTAT996381.4106387572425167No Hit
TAGCTTATCAGACTGATGTTGAC972181.376377272743361No Hit
TAGCTTATCAGACTGATGTTGACT921071.3040175837866728No Hit
TGAGGTAGTAGGTTGTATAGTT768431.0879153939539805No Hit
TATTGCACTTGTCCCGGCCTGT718151.016730788969784No Hit
TAGCTTATCAGACTGATGTTGA649700.9198217553347752No Hit
CGCCTGTCTGAGCGTCGCT581410.8231392439113308No Hit
CATAAAGTAGAAAGCACTACT552730.7825351374883642No Hit
TGTAAACATCCTTGACTGGAAGCT539030.7631391731231396No Hit
AGCAGCATTGTACAGGGCTATGA481480.6816619651509735No Hit
CAACGGAATCCCAAAAGCAGCTGT480670.6805151964549273No Hit
TATTGCACTTGTCCCGGCCTGTA428510.606668955484846No Hit
TGTAAACATCCCCGACTGGAAGCT380940.539321070459026No Hit
CACCCGGCTGTGTGCACATGTGC376900.5336013846170182No Hit
TGAGGTAGTAGATTGTATAGTT374330.5299628715937608No Hit
ACTGGACTTGGAGTCAGAAGGC344490.4877164791369504No Hit
TTCAAGTAATTCAGGATAGGTT333480.47212891945365676No Hit
CAACGGAATCCCAAAAGCAGCT290880.41181738062456424No Hit
CATTGCACTTGTCTCGGTCTGA288640.4086460696626589No Hit
CCCATAAAGTAGAAAGCACT285570.4042996747282619No Hit
CCCATAAAGTAGAAAGCACTAC271910.3849603409159284No Hit
TCGTACGACTCTTAGCGG256360.3629452134794874No Hit
AACATTCAACGCTGTCGGTGAGTT224110.3172868302109842No Hit
TGTCAGTTTGTCAAATACCCCAA211380.29926415675337037No Hit
CAACACCAGTCGATGGGCTGT196430.2780984876102969No Hit
TGAGGTAGTAGTTTGTACAGTT190080.26910838733882414No Hit
TGTAAACATCCTACACTCTCAGCT180620.25571526158006325No Hit
CTTCTCACTACTGCACTTGACTAG180540.2556020004742809No Hit
AACGGAATCCCAAAAGCAGCTG170790.241798303207059No Hit
TTCTCACTACTGCACTTGACTAG163230.23109512871062848No Hit
TGATGTAGTAGGTTGTATAGTT146350.20719703539055614No Hit
TACGCCTGTCTGAGCGTCGCT142830.2022135467361335No Hit
CTACGCCTGTCTGAGCGTCGCT139270.19717342752881964No Hit
CTCCCACTGCTTCACTTGACTAG138640.19628149632078376No Hit
CACTAGATTGTGAGCTCCTGGA136490.19323760410288354No Hit
GCCTGTCTGAGCGTCGCT135280.1915245298779258No Hit
CCCCCCACTGCTAAATTTGACTGG133580.18911773138005117No Hit
ACGCCTGTCTGAGCGTCGCT132810.18802759323689622No Hit
TAGCAGCACGTAAATATTGGC130780.1851535926776695No Hit
TTCAAGTAATCCAGGATAGGCTT130240.18438908021363876No Hit
TATTGCACTTGTCCCGGCCTGTT126510.17910828115653746No Hit
ACCACAGGGTAGAACCACGGAC125710.17797567009871412No Hit
TGAGGTAGTAGTTTGTGCTGTT125420.17756509859025316No Hit
TATTGCACTTGTCCCGGCCTGTAG121610.17217103842736955No Hit
TAGCTTATCAGACTGATGTTGACA121010.17132158013400206No Hit
TCGTTTCCCGGCCAATGCACCA113990.1613829181016023No Hit
GCGTTCGCGCTTTCCCCTG113020.16000962719399148No Hit
CACTAGATTGTGAGCTCCTGGAA112340.15904690779484165No Hit
CCCCCCACTGCTAAATTTGACTGGCT111460.157801035631236No Hit
TGTCAGTTTGTCAAATACCCCA111330.1576169863343397No Hit
TGAGGTAGTAGGTTGTGTGGTT110710.15673921276452663No Hit
TGTAAACATCCCCGACTGGAAGC109060.15440320245776598No Hit
CCCCCACTGCTAAATTTGACTGGCTT103350.14631919103255195No Hit
CAAAGAATTCTCCTTTTGGGCT102560.1452007376129514No Hit
TTCAAGTAATCCAGGATAGGC100550.14235505233017026No Hit
CAACGGAATCCCAAAAGCAGCTGA98080.13885811568914075No Hit
ACCCCCCACTGCTAAATTTGACTG96070.1360124304063596No Hit
ACTGGACTTGGAGTCAGAAGGCT94350.13357731663203945No Hit
AGCAGCATTGTACAGGGCTATGT94160.1333083215058064No Hit
CAAAGAATTCTCCTTTTGGGCTT92880.13149614381328908No Hit
TTCACAGTGGCTAAGTTCCGC91580.12965565084432615No Hit
TATTGCACTTGTCCCGGCCTGTAAT91500.12954238973854382No Hit
ACTGGACTTGGAGTCAGAAGGCA89750.12706480304955528No Hit
ATCCCACCGCTGCCACCA87320.12362449696141689No Hit
TTCACAGTGGCTAAGTTCTGC86330.12222289077736051No Hit
TTATAAAGCAATGAGACTGATT84570.11973114645014919No Hit
AACATTCAACGCTGTCGGTGA84540.11968867353548082No Hit
CAAAGTGCTGTTCGTGCAGGTAG83740.11855606247765749No Hit
AACATTCAACGCTGTCGGTGAGA79290.11225591346851518No Hit
AGCAGCATTGTACAGGGCTATG78950.11177455376894026No Hit
CAAAGTGCTGTTCGTGCAGGT78230.11075520381689927No Hit
TCGTTTCCCGGGCGGCGCACCA74910.10605486792693243No Hit
AGCTCGGTCTGAGGCCCCTCAGT73690.10432763606375184No Hit
TATTGCACTTGTCCCGGCCTGTTT72730.10296850279436386No Hit
TCAGTGCACTACAGAACTTTGT72330.10240219726545217No Hit
ACTGGACTTGGAGTCAGAAGG71800.10165184243964422No Hit
ACTTGACTTGGAGTCAGAAGGC71540.10128374384585163No Hit
TGATGTAGTAGATTGTATAGTT70860.10032102444670181No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)