FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6848329
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG74396810.86349677417659No Hit
CCCCCCACTGCTAAATTTGACTG6086278.887233659481021No Hit
AACATTCAACGCTGTCGGTGAGT3016454.404651120003143No Hit
AAGCTGCCAGTTGAAGAACTGT2308383.3707200690854657No Hit
TAGCAGCACGTAAATATTGGCG2265593.308237673744938No Hit
TTCAAGTAATCCAGGATAGGCT2127423.1064804275612343No Hit
CCCCCACTGCTAAATTTGACTG1989142.9045625582532613No Hit
AACATTCAACGCTGTCGGTGAG1851562.703666836099726No Hit
TATTGCACTTGTCCCGGCCTGTAA1136701.6598209577840084No Hit
TATTGCACTTGTCCCGGCCTGTAT1066761.5576938549535224No Hit
TAGCTTATCAGACTGATGTTGAC1027331.5001177659542932No Hit
TAGCTTATCAGACTGATGTTGACT958001.3988813913583884No Hit
TATTGCACTTGTCCCGGCCTGT798701.1662699032128858No Hit
TGAGGTAGTAGGTTGTATAGTT717091.0471021471077104No Hit
TAGCTTATCAGACTGATGTTGA634700.9267954270304478No Hit
TGTAAACATCCTTGACTGGAAGCT564260.8239382190896495No Hit
AGCAGCATTGTACAGGGCTATGA473610.6915701625900275No Hit
TATTGCACTTGTCCCGGCCTGTA462750.675712279594044No Hit
TCGTACGACTCTTAGCGG457420.6679293591181148No Hit
CAACGGAATCCCAAAAGCAGCTGT436630.6375715886313289No Hit
ACTGGACTTGGAGTCAGAAGGC434340.6342277072260984No Hit
CATAAAGTAGAAAGCACTACT429170.6266784203854692No Hit
TGTAAACATCCCCGACTGGAAGCT417560.6097253797240174No Hit
CGCCTGTCTGAGCGTCGCT403000.5884647189117229No Hit
CACCCGGCTGTGTGCACATGTGC401760.5866540582381483No Hit
TGAGGTAGTAGATTGTATAGTT355360.5189003040011658No Hit
CAACGGAATCCCAAAAGCAGCT322230.4705235393918721No Hit
TTCAAGTAATTCAGGATAGGTT309860.4524607389627455No Hit
CATTGCACTTGTCTCGGTCTGA290820.4246583363620527No Hit
CCCATAAAGTAGAAAGCACTAC274680.40109054340117134No Hit
CCCATAAAGTAGAAAGCACT255670.3733319471070972No Hit
AACATTCAACGCTGTCGGTGAGTT195170.2849892287593076No Hit
CACTAGATTGTGAGCTCCTGGA194640.2842153173423765No Hit
AACGGAATCCCAAAAGCAGCTG189270.2763739884576223No Hit
CAACACCAGTCGATGGGCTGT186930.2729570965413607No Hit
TTAATGCTAATCGTGATAGGGGTT178480.2606183201770826No Hit
TTCTCACTACTGCACTTGACTAG178400.2605015033594327No Hit
TGTAAACATCCTACACTCTCAGCT175770.25666115047918986No Hit
CTTCTCACTACTGCACTTGACTAG168490.2460308200730426No Hit
TGAGGTAGTAGTTTGTACAGTT166180.24265773446339978No Hit
TGTCAGTTTGTCAAATACCCCAA158340.23120968633370273No Hit
TGAGGTAGTAGTTTGTGCTGTT149210.21787796701939993No Hit
TCGTTTCCCGGCCAATGCACCA148220.21643235890098153No Hit
CTCCCACTGCTTCACTTGACTAG145290.21215394295455142No Hit
CACTAGATTGTGAGCTCCTGGAA143360.20933573722874588No Hit
CCCCCACTGCTAAATTTGACTGGCTT136920.1999319834079233No Hit
TATTGCACTTGTCCCGGCCTGTT135970.1985447836983299No Hit
TATTGCACTTGTCCCGGCCTGTAG135410.19772706597478013No Hit
TTCAAGTAATCCAGGATAGGCTT128150.18712593977304537No Hit
CTACGCCTGTCTGAGCGTCGCT127120.18562192324580198No Hit
TAGCTTATCAGACTGATGTTGACA126350.18449756137592105No Hit
CAAAGAATTCTCCTTTTGGGCT125060.18261389019131527No Hit
TACGCCTGTCTGAGCGTCGCT124280.18147492621922806No Hit
TGTAAACATCCCCGACTGGAAGC123910.180934648437597No Hit
TAGCAGCACGTAAATATTGGC122200.17843768896032886No Hit
ACTGGACTTGGAGTCAGAAGGCT116390.1699538675784998No Hit
CAACGGAATCCCAAAAGCAGCTGA116140.16958881502334366No Hit
CAAAGAATTCTCCTTTTGGGCTT115360.16844985105125646No Hit
ACCACAGGGTAGAACCACGGAC114370.16700424293283805No Hit
CCCCCCACTGCTAAATTTGACTGGCT110930.16198111977388938No Hit
ACTGGACTTGGAGTCAGAAGGCA110690.16163066932093945No Hit
CCCCCCACTGCTAAATTTGACTGG109180.15942575188779626No Hit
CACCCGTAGAACCGACCTTGCG104660.152825601690573No Hit
TGAGGTAGTAGGTTGTGTGGTT103430.1510295431192047No Hit
TCAGTGCACTACAGAACTTTGT96840.14140675776528844No Hit
TCGTTTCCCGGGCGGCGCACCA96790.1413337472542572No Hit
ACCGGGTGCTGTAGGCTT96520.14093949049468857No Hit
TTCAAGTAATCCAGGATAGGC94910.13858855203948292No Hit
TGAGAACTGAATTCCATAGGCTGT94330.13774163011152063No Hit
GCGTTCGCGCTTTCCCCTG90980.13284992587242814No Hit
ACTGGACTTGGAGTCAGAAGG90370.13195919763784714No Hit
TATTGCACTTGTCCCGGCCTGTAAT90260.13179857451357843No Hit
TGTCAGTTTGTCAAATACCCCA85410.12471655494354901No Hit
TTCACAGTGGCTAAGTTCTGC85330.12459973812589903No Hit
AGCAGCATTGTACAGGGCTATGT84730.12372361199352426No Hit
ACGCCTGTCTGAGCGTCGCT83380.12175232819568102No Hit
AGCTCGGTCTGAGGCCCCTCAGT82460.12040893479270637No Hit
AACATTCAACGCTGTCGGTGA81370.11881730565222552No Hit
ACCGGGTGCTGTAGGCTTT80580.11766373957793207No Hit
ATCCCGGACGAGCCCCCA80140.11702124708085723No Hit
ACCCCCCACTGCTAAATTTGACTG80000.11681681764996979No Hit
TTCACAGTGGCTAAGTTCCGC79430.11598449782421376No Hit
AGCAGCATTGTACAGGGCTATG78330.11437826658152667No Hit
TATTGCACTTGTCCCGGCCTGTTT77490.113151689996202No Hit
TGAGAACTGAATTCCATGGGTT76940.11234857437485844No Hit
AATGACACGATCACTCCCGTTG76920.11231937017044595No Hit
AATGCACCCGGGCAAGGATTCT76360.11150165244689617No Hit
TTATAAAGCAATGAGACTGATT76250.11134102932262746No Hit
AACATTCAACGCTGTCGGTGAGA75910.11084455784761509No Hit
CAAAGTGCTGTTCGTGCAGGTAG75750.11061092421231515No Hit
CAAAGTGCTGTTCGTGCAGGT71130.10386475299302939No Hit
ACCGGGTGCTGTAGGCTTA70280.10262357430549847No Hit
CTCCCACTGCTTCACTTGACTAGCCT69540.10154301874223624No Hit
TTCACAGTGGCTAAGTTCCG69470.10144080402679251No Hit
CCCATAAAGTAGAAAGCACTA69390.10132398720914255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)