FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003947635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23947808
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG245786510.263423692055657No Hit
CAACGGAATCCCAAAAGCAGCTG21401878.936880569612049No Hit
TAGCAGCACGTAAATATTGGCG8741543.650246402509992No Hit
AAGCTGCCAGTTGAAGAACTGT7864973.284212901656803No Hit
AACATTCAACGCTGTCGGTGAGT7706073.2178602734747166No Hit
TTCAAGTAATCCAGGATAGGCT7685343.2092039488541078No Hit
CCCCCACTGCTAAATTTGACTG7272883.036971066412425No Hit
TATTGCACTTGTCCCGGCCTGTAT6140042.563925683720197No Hit
TATTGCACTTGTCCCGGCCTGTAA5989162.5009220050536567No Hit
TAGCTTATCAGACTGATGTTGACT5212662.176675209689338No Hit
AACATTCAACGCTGTCGGTGAG5027232.0992443233217837No Hit
TAGCTTATCAGACTGATGTTGAC4647341.9406118505710417No Hit
TGTAAACATCCTTGACTGGAAGCT3056301.276233716254949No Hit
TGAGGTAGTAGGTTGTATAGTT2909701.2150172575293738No Hit
TATTGCACTTGTCCCGGCCTGT2518001.0514532269508758No Hit
TAGCTTATCAGACTGATGTTGA2406091.0047224363916731No Hit
TGAGGTAGTAGATTGTATAGTT2069710.8642586411248996No Hit
TATTGCACTTGTCCCGGCCTGTA2047470.854971778627923No Hit
TGTAAACATCCCCGACTGGAAGCT1937980.8092515189699199No Hit
CATAAAGTAGAAAGCACTACT1850870.7728765822742524No Hit
AGCAGCATTGTACAGGGCTATGA1748610.7301753880772721No Hit
CCCATAAAGTAGAAAGCACTAC1486710.6208125603813093No Hit
TTCAAGTAATTCAGGATAGGTT1475220.616014626474373No Hit
CAACGGAATCCCAAAAGCAGCTGT1472340.6148120111869947No Hit
ACTGGACTTGGAGTCAGAAGGC1334740.5573537252344766No Hit
CACCCGGCTGTGTGCACATGTGC1216280.5078878200459933No Hit
TGTAAACATCCTACACTCTCAGCT950220.39678788137937304No Hit
CATTGCACTTGTCTCGGTCTGA934500.39022360626909985No Hit
TGAGAACTGAATTCCATAGGCTGT881720.3681840108288825No Hit
TGAGGTAGTAGTTTGTACAGTT876530.3660167978630863No Hit
CCCATAAAGTAGAAAGCACT873990.36495615799157904No Hit
TTAATGCTAATCGTGATAGGGGTT823310.3437934695317417No Hit
CACTAGATTGTGAGCTCCTGGAA822240.3433466645465004No Hit
TGTCAGTTTGTCAAATACCCCAA822060.3432715010910393No Hit
GCCTGTCTGAGCGTCGCT786230.32830979770674623No Hit
GGAATACCGGGTGCTGTAGGCTTT771430.32212969136883013No Hit
CACTAGATTGTGAGCTCCTGGA763980.3190187594622439No Hit
TATTGCACTTGTCCCGGCCTGTAG760960.31775768370950697No Hit
CAAAGAATTCTCCTTTTGGGCT757070.31613331792204113No Hit
CCCCCACTGCTAAATTTGACTGGCTT732940.30605723914272237No Hit
TAGCTTATCAGACTGATGTTGACA666030.27811731244880533No Hit
TATTGCACTTGTCCCGGCCTGTAAT609310.2544324724834941No Hit
CAAAGAATTCTCCTTTTGGGCTT601580.25120461964619056No Hit
AGCTCGGTCTGAGGCCCCTCAGT597560.24952596914089173No Hit
TCGTACGACTCTTAGCGG592370.24735875617509545No Hit
TTCTCACTACTGCACTTGACTAG590620.2466280003581121No Hit
CTTCTCACTACTGCACTTGACTAG588340.24567592992227097No Hit
TGAGAACTGAATTCCATGGGTT585430.24446078739231583No Hit
AACATTCAACGCTGTCGGTGAGTT579880.24214324751559727No Hit
CCCCCCACTGCTAAATTTGACTGGCT574140.23974636843589192No Hit
TATTGCACTTGTCCCGGCCTGTTT571910.23881517673767888No Hit
TATTGCACTTGTCCCGGCCTGTT561230.23435547838031773No Hit
TGAGGTAGTAGTTTGTGCTGTT533190.22264668231848192No Hit
AACGGAATCCCAAAAGCAGCTG532750.22246294942735467No Hit
TGTCAGTTTGTCAAATACCCCA528910.220859462377517No Hit
TAGCAGCACGTAAATATTGGC524860.21916828462964127No Hit
TTCAAGTAATCCAGGATAGGCTT521080.21758985206495723No Hit
TGTAAACATCCCCGACTGGAAGC515970.21545604507936592No Hit
CGCCTGTCTGAGCGTCGCT514970.215038470326804No Hit
TCGTTTCCCGGGCGGCGCACCA492870.20581006829518594No Hit
GGAATACCGGGTGCTGTAGGCTT491860.20538831779509842No Hit
TATTGCACTTGTCCCGGCCTGTAAA469200.19592607390204564No Hit
ATCCCGGACGAGCCCCCA462910.19329952870843128No Hit
TCAGTGCACTACAGAACTTTGT454420.1897543190591807No Hit
CTCCCACTGCTTCACTTGACTAG435110.1816909505872103No Hit
CCCCCCACTGCTAAATTTGACTGG416240.17381131500636718No Hit
CAACACCAGTCGATGGGCTGT412330.17217859772385014No Hit
ACTGGACTTGGAGTCAGAAGGCA400820.16737231232186262No Hit
ACCCCCCACTGCTAAATTTGACTG392650.16396072659343186No Hit
ACTGGACTTGGAGTCAGAAGGCT380020.158686757468575No Hit
AGCAGCATTGTACAGGGCTATGT356060.14868166639719177No Hit
CCCCCCACTGCTAAATTTGACTGT355580.14848123051596204No Hit
TTCAAGTAATCCAGGATAGGC335590.14013391121224958No Hit
CAACGGAATCCCAAAAGCAGCT329520.1375992324641988No Hit
CCCATAAAGTAGAAAGCACTA315590.1317824161610115No Hit
TGTCAGTTTGTCAAATACCCC304500.12715151215509995No Hit
TCAGTGCACTACAGAACTTTGTC302600.12635812012523234No Hit
TTCACAGTGGCTAAGTTCCGC287730.12014878355463682No Hit
CAGTGCAATAGTATTGTCAAAGC281450.11752641410854806No Hit
TAGCTTATCAGACTGATGTTGACC281030.11735103271247205No Hit
AGCTACATCTGGCTACTGGGTCTC276780.11557634001408397No Hit
TTATAAAGCAATGAGACTGATT272400.11374736259786283No Hit
ATCACATTGCCAGGGATTTCCA269430.11250716558275396No Hit
CAAAGTGCTGTTCGTGCAGGTAG267620.11175135528061692No Hit
ACCACAGGGTAGAACCACGGAC264740.11054873999323864No Hit
ATGCACCTGGGCAAGGATTCTGA260970.10897448317608024No Hit
TGTAAACATCCTACACTCAGCT260470.1087656957997993No Hit
TATTGCACTTGTCCCGGCCTGTATT257920.10770088018076646No Hit
TGAGGGGCAGAGAGCGAGACTTT256590.10714550575985911No Hit
TAGCAGCACATCATGGTTTACA255970.10688660941327072No Hit
ATCACATTGCCAGGGATTTCC255960.10688243366574511No Hit
AACCGGGCGGAAACACCA255310.10661101007657986No Hit
TAGCAGCACGTAAATATTGGCGT250760.1047110449524232No Hit
ACTGGACTTGGAGTCAGAAGG250290.1045147848187191No Hit
GACCCGCGGGCGCTCTCCAGTCCT246600.10297393398176569No Hit
TTCACAGTGGCTAAGTTCTGC242480.10125352600121064No Hit
GAATACCGGGTGCTGTAGGCTTT241120.10068562433772645No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)