FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7841198
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG79413410.127712627585733No Hit
CCCCCCACTGCTAAATTTGACTG7323679.33998860888349No Hit
AACATTCAACGCTGTCGGTGAGT2813693.588341985497624No Hit
TAGCAGCACGTAAATATTGGCG2498313.1861330373241437No Hit
AAGCTGCCAGTTGAAGAACTGT2316392.9541276728377475No Hit
TTCAAGTAATCCAGGATAGGCT2260192.882454951399008No Hit
AACATTCAACGCTGTCGGTGAG1970932.513557239595276No Hit
CCCCCACTGCTAAATTTGACTG1946342.4821972356775075No Hit
TATTGCACTTGTCCCGGCCTGTAA1898032.4205867521774094No Hit
TATTGCACTTGTCCCGGCCTGTAT1886202.4054997718460878No Hit
TAGCTTATCAGACTGATGTTGACT1358961.7331025182631532No Hit
TAGCTTATCAGACTGATGTTGAC1213831.5480160046972413No Hit
TATTGCACTTGTCCCGGCCTGT844181.076595693668238No Hit
TATTGCACTTGTCCCGGCCTGTA766770.977873534120679No Hit
TGTAAACATCCTTGACTGGAAGCT747300.9530431446827384No Hit
TGAGGTAGTAGGTTGTATAGTT734840.937152715694719No Hit
TAGCTTATCAGACTGATGTTGA618030.7881831322203572No Hit
TGTAAACATCCCCGACTGGAAGCT556490.7097002269296094No Hit
AGCAGCATTGTACAGGGCTATGA555860.7088967782729119No Hit
TTAATGCTAATCGTGATAGGGGTT542480.6918330591830484No Hit
CAACGGAATCCCAAAAGCAGCTGT515740.6577311273098829No Hit
TGAGGTAGTAGATTGTATAGTT496940.6337551991417638No Hit
ACTGGACTTGGAGTCAGAAGGC431370.5501327730788076No Hit
CATAAAGTAGAAAGCACTACT426980.5445341387884861No Hit
CCCATAAAGTAGAAAGCACTAC415670.5301103224277719No Hit
TGAGAACTGAATTCCATAGGCTGT405590.5172551439206101No Hit
TTCAAGTAATTCAGGATAGGTT393610.5019768662900745No Hit
CACCCGGCTGTGTGCACATGTGC381340.48632874721439245No Hit
TCGTACGACTCTTAGCGG360130.4592793091055729No Hit
CATTGCACTTGTCTCGGTCTGA279840.3568842414131106No Hit
CACTAGATTGTGAGCTCCTGGAA271000.3456104539127822No Hit
GCCTGTCTGAGCGTCGCT270150.34452643588390447No Hit
CCCCCACTGCTAAATTTGACTGGCTT252370.32185132935043853No Hit
TATTGCACTTGTCCCGGCCTGTAG252160.3215835131315393No Hit
CAAAGAATTCTCCTTTTGGGCT251540.3207928176281226No Hit
AACATTCAACGCTGTCGGTGAGTT248100.31640573289948803No Hit
CACTAGATTGTGAGCTCCTGGA243820.3109473832952567No Hit
TGTAAACATCCTACACTCTCAGCT217850.2778274442247218No Hit
TGAGGTAGTAGTTTGTACAGTT217640.27755962800582257No Hit
AGCTCGGTCTGAGGCCCCTCAGT216520.2761312748383602No Hit
TCGTTTCCCGGGCGGCGCACCA216280.27582519915961823No Hit
ATCCCGGACGAGCCCCCA209340.26697451078266354No Hit
CCCATAAAGTAGAAAGCACT208350.265711948607853No Hit
TATTGCACTTGTCCCGGCCTGTT203970.2601260674708125No Hit
GGAATACCGGGTGCTGTAGGCTTT195790.24969398808702445No Hit
TGTCAGTTTGTCAAATACCCCAA192450.24543443489119904No Hit
TAGCTTATCAGACTGATGTTGACA184160.2348620708213209No Hit
AACGGAATCCCAAAAGCAGCTG183950.23459425460242173No Hit
TATTGCACTTGTCCCGGCCTGTAAT179910.22944198067693225No Hit
TGAGAACTGAATTCCATGGGTT177850.2268148311010639No Hit
TATTGCACTTGTCCCGGCCTGTTT175780.2241749283719146No Hit
CCCCCCACTGCTAAATTTGACTGGCT175770.22416217521863369No Hit
TTCTCACTACTGCACTTGACTAG173370.22110141843121423No Hit
TAGCAGCACGTAAATATTGGC165410.21094990841960629No Hit
ACTGGACTTGGAGTCAGAAGGCA157430.20077289210143653No Hit
AGCTACATCTGGCTACTGGGTCTC157280.20058159480222282No Hit
TATTGCACTTGTCCCGGCCTGTAAA156970.2001862470505145No Hit
CGCCTGTCTGAGCGTCGCT155040.197724888467298No Hit
TGAGGTAGTAGTTTGTGCTGTT153830.19618175692030734No Hit
CTTCTCACTACTGCACTTGACTAG153650.19595220016125087No Hit
CAACACCAGTCGATGGGCTGT149610.19079992623576142No Hit
CAAAGAATTCTCCTTTTGGGCTT148480.18935881991501807No Hit
TGTAAACATCCCCGACTGGAAGC139410.17779170988922866No Hit
ACTGGACTTGGAGTCAGAAGGCT137120.17487123778789926No Hit
TTCACAGTGGCTAAGTTCCG135160.17237161974484No Hit
ACCCCCCACTGCTAAATTTGACTG133790.17062443774535474No Hit
CAACGGAATCCCAAAAGCAGCT133210.1698847548550617No Hit
TTCAAGTAATCCAGGATAGGCTT131760.1680355476293291No Hit
CCCCCCACTGCTAAATTTGACTGG126650.1615186863027818No Hit
GGAATACCGGGTGCTGTAGGCTT126140.16086827548545515No Hit
CTCCCACTGCTTCACTTGACTAG118320.15089530961978004No Hit
TGTCAGTTTGTCAAATACCCCA117710.15011736726964425No Hit
TTCAAGTAATCCAGGATAGGC107040.13650975271890853No Hit
AGCAGCATTGTACAGGGCTATGT104430.13318117971258983No Hit
AACCGGGCGGAAACACCA101260.12913843012253995No Hit
ACCGGGTGCTGTAGGCTTA100630.12833498146584235No Hit
TTAATGCTAATCGTGATAGGGGTTT99410.12677909676557078No Hit
CAAAGTGCTGTTCGTGCAGGTAG95700.1220476768983515No Hit
CCCATAAAGTAGAAAGCACTA94390.12037701381855172No Hit
TGAGGTAGTAGGTTGTGTGGTT93530.11928024263639307No Hit
ACTGGACTTGGAGTCAGAAGG91930.11723973811144674No Hit
TGAGGGGCAGAGAGCGAGACTTT91680.11692090927942389No Hit
ACCACAGGGTAGAACCACGGAC90420.11531401196602867No Hit
AGCTACATCTGGCTACTGGGTCTCT89670.11435752546996009No Hit
CCCCCCACTGCTAAATTTGACTGT88030.1122660083318901No Hit
TCAGTGCACTACAGAACTTTGT84880.10824876504840204No Hit
ATCACATTGCCAGGGATTTCCA82920.10574914700534281No Hit
TTCACCACCTTCTCCACCCAGC82290.1049456983486452No Hit
ATGCACCTGGGCAAGGATTCTGA81770.10428253437803764No Hit
AACATTCAACGCTGTCGGTGAGA81490.10392544608617205No Hit
AGCTACATTGTCTGCTGGGTTT81480.10391269293289111No Hit
ATCACATTGCCAGGGATTTCC79520.10141307488983188No Hit
CTACGCCTGTCTGAGCGTCGCT79400.10126003705046092No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)