FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8522961
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7784359.133386859332102No Hit
CCCCCCACTGCTAAATTTGACTG6794747.972276301628037No Hit
AACATTCAACGCTGTCGGTGAGT3064493.5955696617642627No Hit
TTCAAGTAATCCAGGATAGGCT2795693.280186310837278No Hit
TAGCAGCACGTAAATATTGGCG2700043.1679600551967795No Hit
AAGCTGCCAGTTGAAGAACTGT2409722.827327263377129No Hit
CCCCCACTGCTAAATTTGACTG2175122.5520708120100517No Hit
AACATTCAACGCTGTCGGTGAG1937232.272954199837357No Hit
TATTGCACTTGTCCCGGCCTGTAA1844522.1641774495976223No Hit
TATTGCACTTGTCCCGGCCTGTAT1708132.0041509048322528No Hit
TAGCTTATCAGACTGATGTTGACT1584181.8587202264565097No Hit
TAGCTTATCAGACTGATGTTGAC1536731.8030470865700314No Hit
TGTAAACATCCTTGACTGGAAGCT1019371.1960280001281245No Hit
TGAGGTAGTAGGTTGTATAGTT923411.0834380211290418No Hit
TAGCTTATCAGACTGATGTTGA848370.995393502328592No Hit
TATTGCACTTGTCCCGGCCTGT731440.8581993980730406No Hit
TATTGCACTTGTCCCGGCCTGTA681710.799851131549235No Hit
CATAAAGTAGAAAGCACTACT677110.794453946228312No Hit
AGCAGCATTGTACAGGGCTATGA646110.7580816103699173No Hit
TGTAAACATCCCCGACTGGAAGCT627400.7361291457276409No Hit
TGAGGTAGTAGATTGTATAGTT613860.7202426480656194No Hit
CCCATAAAGTAGAAAGCACTAC595100.698231518365507No Hit
CAACGGAATCCCAAAAGCAGCTGT522120.6126039999479054No Hit
CCCCCACTGCTAAATTTGACTGGCTT471180.5528360390244658No Hit
ACTGGACTTGGAGTCAGAAGGC464530.5450335863322618No Hit
TTCAAGTAATTCAGGATAGGTT462000.5420651344057541No Hit
CACCCGGCTGTGTGCACATGTGC414690.48655625668121677No Hit
CAACGGAATCCCAAAAGCAGCT398760.4678655692546288No Hit
GCCTGTCTGAGCGTCGCT334670.39266869812028943No Hit
TGTAAACATCCTACACTCTCAGCT294740.3458187829323635No Hit
TTCTCACTACTGCACTTGACTAG294390.3454081275275107No Hit
CAAAGAATTCTCCTTTTGGGCT294050.34500920513422506No Hit
CTTCTCACTACTGCACTTGACTAG291010.34144236961778895No Hit
TGTCAGTTTGTCAAATACCCCAA287200.3369720922106766No Hit
CATTGCACTTGTCTCGGTCTGA282620.3315983729128879No Hit
CCCATAAAGTAGAAAGCACT280480.32908750843750195No Hit
TGAGGTAGTAGTTTGTACAGTT269200.3158526713896731No Hit
CCCCCCACTGCTAAATTTGACTGGCT268160.31463243818668185No Hit
TAGCTTATCAGACTGATGTTGACA257050.3015970623354959No Hit
AACATTCAACGCTGTCGGTGAGTT255020.29921526098734935No Hit
TCGTACGACTCTTAGCGG238050.27930434035777No Hit
TCGTTTCCCGGCCAATGCACCA229650.26944861064130177No Hit
CACTAGATTGTGAGCTCCTGGAA213650.2506757921337432No Hit
CTCCCACTGCTTCACTTGACTAG204060.23942383404077527No Hit
TATTGCACTTGTCCCGGCCTGTAG198590.23300587671350367No Hit
TGTCAGTTTGTCAAATACCCCA196430.23047154621498328No Hit
CAAAGAATTCTCCTTTTGGGCTT191590.2247927686164468No Hit
CACTAGATTGTGAGCTCCTGGA186270.21855080646268357No Hit
TGTAAACATCCCCGACTGGAAGC175940.20643060551374104No Hit
TTCAAGTAATCCAGGATAGGCTT175030.20536290146112365No Hit
TATTGCACTTGTCCCGGCCTGTT174760.2050461101488086No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC174040.2042013333159685No Hit
AACGGAATCCCAAAAGCAGCTG171250.20092782308871296No Hit
TAGCAGCACGTAAATATTGGC164070.19250352078344604No Hit
TATTGCACTTGTCCCGGCCTGTTT162470.19062623893269018No Hit
TGAGGTAGTAGTTTGTGCTGTT160870.18874895708193432No Hit
CTCCCACTGCTTCACTTGACTAGCCT159340.18695380631214903No Hit
TATTGCACTTGTCCCGGCCTGTAAT153020.1795385430016634No Hit
TATTGCACTTGTCCCGGCCTGTAAA150510.17659355709829014No Hit
ACTGGACTTGGAGTCAGAAGGCA148310.17401229455350084No Hit
ACCCCCCACTGCTAAATTTGACTG143660.16855644417474164No Hit
TTCACAGTGGCTAAGTTCCG142050.16666742931241854No Hit
CCCCCCACTGCTAAATTTGACTGG141250.1657287883870406No Hit
CAACACCAGTCGATGGGCTGT138740.16278380248366733No Hit
GTCAGGATGGCCGAGCGGTCTAAGG138590.16260780731015897No Hit
ACTGGACTTGGAGTCAGAAGGCT136790.16049586522805867No Hit
AGCAGCATTGTACAGGGCTATGT135550.15904097179372287No Hit
TCGTTTCCCGGGCGGCGCACCA128140.15034681022240978No Hit
CAACGGAATCCCAAAAGCAGCTGA128090.15028814516457367No Hit
CCCATAAAGTAGAAAGCACTA127530.14963109651680914No Hit
TTCAAGTAATCCAGGATAGGC121130.1421219691137857No Hit
TTCACAGTGGCTAAGTTCTGC115810.13588000696002245No Hit
TTCACAGTGGCTAAGTTCCGC114680.13455417665292613No Hit
TCAGTGCACTACAGAACTTTGT113120.13272382684843917No Hit
ATGCACCTGGGCAAGGATTCTGA112610.13212544325851075No Hit
AGCTCGGTCTGAGGCCCCTCAGT112100.13152705966858233No Hit
CCCCCACTGCTAAATTTGACTGGCTA111850.13123373437940172No Hit
CAAAGTGCTGTTCGTGCAGGTAG108960.12784289403647395No Hit
GGAATACCGGGTGCTGTAGGCTTT108870.1277372969323689No Hit
TTATAAAGCAATGAGACTGATT108220.12697465118049936No Hit
ATCACATTGCCAGGGATTTCCA106840.12535549558422243No Hit
CGCCTGTCTGAGCGTCGCT104450.12255130581965586No Hit
ACCACAGGGTAGAACCACGGAC101700.11932472763866923No Hit
ATCACATTGCCAGGGATTTCC100190.1175530428920184No Hit
TAGCAGCACATCATGGTTTACA99350.11656746992037158No Hit
TAGCAGCACGTAAATATTGGCGT98810.11593388729574147No Hit
TGATGTAGTAGGTTGTATAGTT97820.11477231915058628No Hit
ACTGGACTTGGAGTCAGAAGG97650.11457285795394347No Hit
CCCCCACTGCTAAATTTGACTGGCT97030.11384541123677558No Hit
CTACGGGGATGATTTTACGAACTGAA95530.11208545950169195No Hit
TTCACAGTGGCTAAGTTCTG89180.10463499715650465No Hit
CTACGCCTGTCTGAGCGTCGCT89130.10457633209866853No Hit
TGTCAGTTTGTCAAATACCCC88250.1035438270807528No Hit
CAAAGTGCTGTTCGTGCAGGT86460.10144361801021969No Hit
TGTAAACATCCTACACTCAGCT85230.10000045758745112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)