FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12100526
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG245369920.277622642189275No Hit
CAACGGAATCCCAAAAGCAGCTG7825666.4672064668924305No Hit
CCCCCACTGCTAAATTTGACTG6807805.626036421887775No Hit
TTCAAGTAATCCAGGATAGGCT3411622.8193980988925604No Hit
TAGCAGCACGTAAATATTGGCG3160392.6117790251432043No Hit
AACATTCAACGCTGTCGGTGAGT2923682.4161594297636317No Hit
AAGCTGCCAGTTGAAGAACTGT2882982.3825245282725724No Hit
TAGCTTATCAGACTGATGTTGAC2096581.7326354242782505No Hit
TAGCTTATCAGACTGATGTTGACT1989101.643812839210461No Hit
AACATTCAACGCTGTCGGTGAG1681761.3898238803833818No Hit
TATTGCACTTGTCCCGGCCTGTAT1492801.2336653795049901No Hit
TATTGCACTTGTCCCGGCCTGTAA1482761.2253682195302915No Hit
TAGCTTATCAGACTGATGTTGA1463361.2093358586230052No Hit
CATAAAGTAGAAAGCACTACT1200700.9922709145040471No Hit
TGTAAACATCCTTGACTGGAAGCT1036870.8568801058730835No Hit
TATTGCACTTGTCCCGGCCTGT887390.7333482858513753No Hit
TGAGGTAGTAGGTTGTATAGTT886460.7325797242202529No Hit
TGAGGTAGTAGATTGTATAGTT781320.6456909393856102No Hit
TCGTACGACTCTTAGCGG750640.6203366696621287No Hit
TATTGCACTTGTCCCGGCCTGTA717720.5931312407411049No Hit
TTCAAGTAATTCAGGATAGGTT683700.565016760428431No Hit
AGCAGCATTGTACAGGGCTATGA668420.552389210188053No Hit
CCCATAAAGTAGAAAGCACTAC585780.48409465836443805No Hit
ACTGGACTTGGAGTCAGAAGGC564070.4661532895346864No Hit
GACCCGCGGGCGCTCTCCAGTCCT557570.4607816222203894No Hit
TGTAAACATCCCCGACTGGAAGCT506800.4188247684439502No Hit
CAACGGAATCCCAAAAGCAGCTGT496710.4104862879514494No Hit
TTCTCACTACTGCACTTGACTAG490910.4056931078863844No Hit
CTTCTCACTACTGCACTTGACTAG464160.3835866308621625No Hit
CATTGCACTTGTCTCGGTCTGA404690.334440006988126No Hit
TGTAAACATCCTACACTCTCAGCT402300.3324648862371768No Hit
CCCATAAAGTAGAAAGCACT362560.2996233386879215No Hit
TGTCAGTTTGTCAAATACCCCAA351230.29026010935392393No Hit
CAAAGAATTCTCCTTTTGGGCT316590.26163325462050163No Hit
CAACGGAATCCCAAAAGCAGCT307630.25422861783033235No Hit
CCCCCCACTGCTAAATTTGACTGG290470.24004741612058847No Hit
TGAGGTAGTAGTTTGTACAGTT286920.23711365935662632No Hit
CTCCCACTGCTTCACTTGACTAG264370.21847810582779625No Hit
TAGCTTATCAGACTGATGTTGACA259190.21419730018347963No Hit
CACTAGATTGTGAGCTCCTGGA258040.2132469282740271No Hit
TGTCAGTTTGTCAAATACCCCA256100.21164369218329848No Hit
CACTAGATTGTGAGCTCCTGGAA255840.2114288254907266No Hit
CCCCCACTGCTAAATTTGACTGGCTT253880.20980906119287707No Hit
ACCCCCCACTGCTAAATTTGACTG252440.20861903028017129No Hit
CCCCCCACTGCTAAATTTGACTGGCT248170.20509025805985626No Hit
CAAAGAATTCTCCTTTTGGGCTT237520.19628898776796974No Hit
CACCCGGCTGTGTGCACATGTGC224800.18577704803906872No Hit
CGCCTGTCTGAGCGTCGCT220190.18196729629769814No Hit
AGCTCGGTCTGAGGCCCCTCAGT220180.18195903219413767No Hit
AACATTCAACGCTGTCGGTGAGTT218950.18094254745620147No Hit
TTCAAGTAATCCAGGATAGGCTT218900.18090122693839922No Hit
AACCGGGCGGAAACACCA214720.17744683165012826No Hit
CCCCCCACTGCTAAATTTGACTGA214500.1772650213717982No Hit
CAACACCAGTCGATGGGCTGT206660.1707859641804001No Hit
TATTGCACTTGTCCCGGCCTGTT186490.15411726729895875No Hit
TAGCAGCACGTAAATATTGGC178020.14711757158325184No Hit
AACGGAATCCCAAAAGCAGCTG176400.14577878680645784No Hit
TATTGCACTTGTCCCGGCCTGTAG173690.14353921474157405No Hit
TTATAAAGCAATGAGACTGATT173450.1433408762561231No Hit
CTTCTCACTACTGCACTTGACTA171350.14160541450842715No Hit
TCAGTGCACTACAGAACTTTGT164050.13557261890929367No Hit
CCCCCCACTGCTAAATTTGACTGT157490.13015136697363405No Hit
TGAGGTAGTAGTTTGTGCTGTT156920.129680313070688No Hit
ACTGGACTTGGAGTCAGAAGGCA153820.1271184409669464No Hit
TGTAAACATCCCCGACTGGAAGC153470.12682919734233042No Hit
TGTAAACATCCTACACTCAGCT151680.12534992280500865No Hit
TTCAAGTAATCCAGGATAGGC151320.12505241507683218No Hit
TATTGCACTTGTCCCGGCCTGTTT146410.12099474022864791No Hit
CTCCCACTGCTTCACTTGACTAGCCT146070.12071376070759239No Hit
AGCAGCATTGTACAGGGCTATGT145250.12003610421563492No Hit
CTACGCCTGTCTGAGCGTCGCT145190.11998651959427217No Hit
CCCCCCACTGCTAAATTTGAC144440.11936671182723793No Hit
ACTGGACTTGGAGTCAGAAGGCT142330.11762298597598154No Hit
TTCACAGTGGCTAAGTTCCG135890.11230090328304737No Hit
CCCATAAAGTAGAAAGCACTA132710.1096729183508221No Hit
ACCGGGTGCTGTAGGCTTA131120.10835892588470948No Hit
TGTCAGTTTGTCAAATACCCC130650.107970513017368No Hit
TTCACAGTGGCTAAGTTCTGC129920.10736723345745466No Hit
TATTGCACTTGTCCCGGCCTGTAAT128360.10607803330202341No Hit
TTCACAGTGGCTAAGTTCCGC127440.10531773577446137No Hit
TCGTTTCCCGGGCGGCGCACCA121410.10033448132750593No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)