FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9462175
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG143101915.123573596979552No Hit
CCCCCCACTGCTAAATTTGACTG139243814.715834361550067No Hit
CCCCCACTGCTAAATTTGACTG5745396.071954915228265No Hit
TTCAAGTAATCCAGGATAGGCT3147733.3266453008954073No Hit
TAGCAGCACGTAAATATTGGCG2495172.636994137183047No Hit
AACATTCAACGCTGTCGGTGAG1845081.9499533669584426No Hit
TCGTACGACTCTTAGCGG1796891.8990242729604982No Hit
AAGCTGCCAGTTGAAGAACTGT1632891.725702600089303No Hit
TGAGGTAGTAGATTGTATAGTT1291771.3651935205172172No Hit
TAGCTTATCAGACTGATGTTGAC1208381.2770636772200896No Hit
TAGCTTATCAGACTGATGTTGA945360.9990937601555668No Hit
TATTGCACTTGTCCCGGCCTGT943410.9970329231915496No Hit
TGAGGTAGTAGGTTGTATAGTT847030.8951747351956606No Hit
TAGCTTATCAGACTGATGTTGACT750850.7935279150935171No Hit
AACATTCAACGCTGTCGGTGAGT730560.7720846422730503No Hit
CAACGGAATCCCAAAAGCAGCTGT633180.6691696148084346No Hit
TATTGCACTTGTCCCGGCCTGTAA626210.6618034437114088No Hit
TTCTCACTACTGCACTTGACTAG579760.612713250389049No Hit
TATTGCACTTGTCCCGGCCTGTAT564460.596543606517529No Hit
TTCAAGTAATTCAGGATAGGTT517130.5465233944626896No Hit
TGAGAACTGAATTCCATGGGTT497630.5259150248225171No Hit
AGCAGCATTGTACAGGGCTATGA488200.5159490286324233No Hit
TGTAAACATCCTTGACTGGAAGCT476660.5037531011633161No Hit
TATTGCACTTGTCCCGGCCTGTA474750.5017345377780478No Hit
CATAAAGTAGAAAGCACTACT451640.4773109776557715No Hit
GCCTGTCTGAGCGTCGCT442440.46758805454348495No Hit
TGAGGTAGTAGTTTGTACAGTT403350.4262761997109544No Hit
CAACGGAATCCCAAAAGCAGCT374460.3957441074594371No Hit
CATTGCACTTGTCTCGGTCTGA355540.37574870471112615No Hit
TGATGTAGTAGATTGTATAGTT332980.35190640629664954No Hit
CCCATAAAGTAGAAAGCACTAC312920.33070620655398997No Hit
AACGGAATCCCAAAAGCAGCTG295540.3123383365875182No Hit
TGAGAACTGAATTCCATAGGCTGT283210.2993075059381167No Hit
CTTCTCACTACTGCACTTGACTAG276970.29271282765326156No Hit
ACTGGACTTGGAGTCAGAAGGC271200.2866148639187079No Hit
CCCCCACTGCTAAATTTGACTGGCTT270040.28538893013498484No Hit
CCCATAAAGTAGAAAGCACT268250.28349718748596386No Hit
CTCCCACTGCTTCACTTGACTAG247930.2620222094814353No Hit
TGTAAACATCCCCGACTGGAAGCT247650.2617262944301918No Hit
CACCCGGCTGTGTGCACATGTGC245680.2596443206768No Hit
GACCCGCGGGCGCTCTCCAGTCCT242380.2561567504300016No Hit
TAGCAGCACGTAAATATTGGC229780.24284057312404392No Hit
TGATGTAGTAGGTTGTATAGTT216900.2292284807668427No Hit
CCCCCCACTGCTAAATTTGACTGG209720.22164037338138431No Hit
TTAATGCTAATCGTGATAGGGGTT196240.20739417734294704No Hit
ACCCCCCACTGCTAAATTTGACTG187840.19851672580564195No Hit
TTCACAGTGGCTAAGTTCCGC179590.18979780018864584No Hit
TAGCTTATCAGACTGATGTTGACA156800.1657124286963621No Hit
CAACACCAGTCGATGGGCTGT156380.16526855611949684No Hit
CCCACCACTGCTAAATTTGACTG139700.14764047378113382No Hit
CAAAGAATTCTCCTTTTGGGCT139280.14719660120426858No Hit
TTCACAGTGGCTAAGTTCTGC137930.14576986792148738No Hit
TGTCAGTTTGTCAAATACCCCA137870.14570645755336378No Hit
TATTGCACTTGTCCCGGCCTGTT137790.14562191039586564No Hit
TGAGGTAGTAGATTGTATAGT137390.14519917460837492No Hit
CTCCCACTGCTTCACTTGACTAGCCT136330.14407892477152454No Hit
TCGTTTCCCGGCCAATGCACCA134190.14181728830844917No Hit
CCCATAAAGTAGAAAGCACTA132950.14050680736722795No Hit
TGTAAACATCCTACACTCTCAGCT132040.13954508345068653No Hit
CCACCCACTGCTAAATTTGACTG130990.1384354020085234No Hit
CACCCCACTGCTAAATTTGACTG129930.137315152171673No Hit
TTCAAGTAATCCAGGATAGGC127790.13505351570859767No Hit
CACTAGATTGTGAGCTCCTGGA125650.1327918792455223No Hit
CCCCCCACTGCTAAATTTGACTGGCT124680.1317667449608573No Hit
CCCCCCACTGCTAAATTTTACTG123110.1301075069949562No Hit
CAAAGTGCTGTTCGTGCAGGTAG122510.12947340331372015No Hit
AGCTCGGTCTGAGGCCCCTCAGT121160.12804667003093897No Hit
TGATAACTGAATTCCATGGGTT120090.1269158517994013No Hit
TGGCTCAGTTCAGCAGGAAC119580.12637686367035064No Hit
TCCTGTCTGAGCGTCGCT117110.12376647018259544No Hit
TTCAAGTAATCCAGGATAGGCTT109200.11540686998496646No Hit
TGAGGTAGTAAGTTGTATTGTT107560.1136736532562545No Hit
AACCGGGCGGAAACACCA106640.11270136094502585No Hit
TGTAAACATCCCCGACTGGA103740.10963652648571813No Hit
TGATGTAGTAGTTTGTACAGTT102290.10810410925606428No Hit
ACCCCCACTGCTAAATTTGACTG101670.10744886878545365No Hit
CAACGGAATCCAAAAAGCAGCTG101120.10686760707765393No Hit
TGAGGTAGTAGTTTGTGCTGTT99040.10466938098270219No Hit
CCCCCAACTGCTAAATTTGACTG97350.10288332228055388No Hit
AATGCACCCGGGCAAGGATTCT96240.10171023047026714No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)