FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9854369
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG197052419.9964503054432No Hit
CAACGGAATCCCAAAAGCAGCTG7768747.883548911147939No Hit
CCCCCACTGCTAAATTTGACTG5632485.715718581270907No Hit
AACATTCAACGCTGTCGGTGAGT3086263.13186973209548No Hit
AAGCTGCCAGTTGAAGAACTGT2706802.746801951499888No Hit
TTCAAGTAATCCAGGATAGGCT2526072.5634010660652145No Hit
TAGCAGCACGTAAATATTGGCG2439892.475947470609229No Hit
AACATTCAACGCTGTCGGTGAG1695541.7205972295131224No Hit
TAGCTTATCAGACTGATGTTGAC1298661.317852010615799No Hit
TAGCTTATCAGACTGATGTTGACT1255041.2735873803792004No Hit
TATTGCACTTGTCCCGGCCTGTAA1217011.2349953609409186No Hit
TATTGCACTTGTCCCGGCCTGTAT1028221.04341536226216No Hit
TGAGGTAGTAGGTTGTATAGTT919520.9331089590820072No Hit
TGTAAACATCCTTGACTGGAAGCT861760.8744953634271255No Hit
TAGCTTATCAGACTGATGTTGA746300.7573290588164499No Hit
TATTGCACTTGTCCCGGCCTGT672180.6821136898770485No Hit
AGCAGCATTGTACAGGGCTATGA576700.5852226560625039No Hit
CATAAAGTAGAAAGCACTACT550570.558706498609906No Hit
CAACGGAATCCCAAAAGCAGCTGT534730.542632410050811No Hit
ACTGGACTTGGAGTCAGAAGGC532210.5400751686891369No Hit
TGAGGTAGTAGATTGTATAGTT520560.5282530012829842No Hit
TATTGCACTTGTCCCGGCCTGTA511920.519485316614387No Hit
TGTAAACATCCCCGACTGGAAGCT493080.500366893100918No Hit
GACCCGCGGGCGCTCTCCAGTCCT482540.4896711296278838No Hit
CCCATAAAGTAGAAAGCACTAC457780.4645452184711167No Hit
TTCAAGTAATTCAGGATAGGTT353920.3591503423506873No Hit
TCGTACGACTCTTAGCGG331990.336896253834213No Hit
TGTAAACATCCTACACTCTCAGCT324040.3288287662051218No Hit
CGCCTGTCTGAGCGTCGCT300940.30538738705644164No Hit
CCCCCACTGCTAAATTTGACTGGCTT289640.2939203920616328No Hit
TTCTCACTACTGCACTTGACTAG282930.2871112295470162No Hit
TTAATGCTAATCGTGATAGGGGTT272550.27657783060488195No Hit
CCCATAAAGTAGAAAGCACT264160.2680638405158159No Hit
TGTCAGTTTGTCAAATACCCCAA243180.24677379140156003No Hit
CATTGCACTTGTCTCGGTCTGA238220.24174049094366165No Hit
AACATTCAACGCTGTCGGTGAGTT234230.23769152545434416No Hit
TGAGGTAGTAGTTTGTACAGTT232990.23643320033986956No Hit
CTTCTCACTACTGCACTTGACTAG225010.2283352693612346No Hit
CCCCCCACTGCTAAATTTGACTGG219210.22244955511611145No Hit
CCCCCCACTGCTAAATTTGACTGGCT210260.21336728916889555No Hit
ACCCCCCACTGCTAAATTTGACTG209940.21304256010709566No Hit
CAAAGAATTCTCCTTTTGGGCT201580.20455901336757332No Hit
AACGGAATCCCAAAAGCAGCTG196190.1990893582328813No Hit
TAGCTTATCAGACTGATGTTGACA188750.19153940754603366No Hit
CAACGGAATCCCAAAAGCAGCT187520.1902912302147403No Hit
CCCCCCACTGCTAAATTTTACTG175740.17833714162723155No Hit
CACCCGGCTGTGTGCACATGTGC164740.16717458012786005No Hit
CCCACCACTGCTAAATTTGACTG162630.16503339787661697No Hit
AGCTCGGTCTGAGGCCCCTCAGT159450.16180640282498046No Hit
CAAAGAATTCTCCTTTTGGGCTT158430.16077132894049329No Hit
CTACGCCTGTCTGAGCGTCGCT158350.16069014667504333No Hit
CACCCCACTGCTAAATTTGACTG154380.15666147675208836No Hit
TGTCAGTTTGTCAAATACCCCA152010.15425645213813285No Hit
CCACCCACTGCTAAATTTGACTG147710.1498929053701967No Hit
TACCCGCGGGCGCTCTCCAGTCCT145040.14718344726080382No Hit
TTCAAGTAATCCAGGATAGGCTT144830.14697034381399762No Hit
TAGCAGCACGTAAATATTGGC141250.14333743743511126No Hit
CACTAGATTGTGAGCTCCTGGA140690.14276916157696143No Hit
TGAGGTAGTAGTTTGTGCTGTT140470.142545910346974No Hit
ACTGGACTTGGAGTCAGAAGGCA140470.142545910346974No Hit
TACGCCTGTCTGAGCGTCGCT139770.14183556552428675No Hit
CACTAGATTGTGAGCTCCTGGAA139710.14177467882519926No Hit
TGTAAACATCCCCGACTGGAAGC137460.13949142760941874No Hit
TATTGCACTTGTCCCGGCCTGTAG136980.13900433401671888No Hit
ACTGGACTTGGAGTCAGAAGGCT131580.1335245310988456No Hit
TATTGCACTTGTCCCGGCCTGTT128640.13054108284355903No Hit
AACCGGGCGGAAACACCA128390.13028738826402786No Hit
CAACACCAGTCGATGGGCTGT126160.12802443261460983No Hit
TGAGGTAGTAGGTTGTGTGGTT120440.12221990063493665No Hit
CCCCACACTGCTAAATTTGACTG119580.12134719128134941No Hit
CCCCCAACTGCTAAATTTGACTG115970.11768384155291932No Hit
CTCCCACTGCTTCACTTGACTAG112160.11381753616086428No Hit
ACCCCCACTGCTAAATTTGACTG107790.10938295491065943No Hit
AGCAGCATTGTACAGGGCTATGT105830.10739398940713504No Hit
TATTGCACTTGTCCCGGCCTGTAAT104240.1057804918813168No Hit
ACCACAGGGTAGAACCACGGAC101820.10332472835145508No Hit
CCCCCCACTGCTAAATTTGACTGA100910.1024012800819616No Hit
CTCCCACTGCTTCACTTGACTAGCCT100790.10227950668378666No Hit
TGTAAACATCCTACACTCAGCT100520.102005516537893No Hit
TTCAAGTAATCCAGGATAGGC98980.10044275792798099No Hit
CCCCCCACTGCTAAATTTGACTGTA98740.10019921113163105No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)