FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8609386
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG89577910.404679265164786No Hit
CAACGGAATCCCAAAAGCAGCTG5030645.843204149517748No Hit
CCCCCACTGCTAAATTTGACTG3851744.473884664945909No Hit
GCCTGTCTGAGCGTCGCT3221153.7414398657465235No Hit
TTCAAGTAATCCAGGATAGGCT2632713.0579532617076293No Hit
AACATTCAACGCTGTCGGTGAGT2502342.9065255060000794No Hit
AAGCTGCCAGTTGAAGAACTGT2457192.8540827417890196No Hit
TAGCAGCACGTAAATATTGGCG2422972.8143354241521985No Hit
TAGCTTATCAGACTGATGTTGAC1356951.5761286577230944No Hit
AACATTCAACGCTGTCGGTGAG1296231.5056009801395824No Hit
TCCTGTCTGAGCGTCGCT1289031.4972380144182176No Hit
CATAAAGTAGAAAGCACTACT1182451.3734428912816778No Hit
TAGCTTATCAGACTGATGTTGA1166071.3544171442655726No Hit
TGAGGTAGTAGGTTGTATAGTT1111311.290812143862524No Hit
TAGCTTATCAGACTGATGTTGACT1060111.2313421653994838No Hit
CGCCTGTCTGAGCGTCGCT829580.9635762643236114No Hit
TGTAAACATCCTTGACTGGAAGCT671060.779451635691558No Hit
TGAGGTAGTAGATTGTATAGTT630780.7326654885725881No Hit
TATTGCACTTGTCCCGGCCTGT627470.7288208473867939No Hit
TATTGCACTTGTCCCGGCCTGTAA606860.7048818580093865No Hit
TATTGCACTTGTCCCGGCCTGTAT523440.6079876079432378No Hit
AGCAGCATTGTACAGGGCTATGA481200.5589248757112295No Hit
TCGTACGACTCTTAGCGG441490.5128007967118677No Hit
ACTGGACTTGGAGTCAGAAGGC426110.49493657271261854No Hit
TTCAAGTAATTCAGGATAGGTT405190.4706375111999857No Hit
TATTGCACTTGTCCCGGCCTGTA378620.439775844642115No Hit
CCCATAAAGTAGAAAGCACTAC378120.4391950831336869No Hit
CCCATAAAGTAGAAAGCACT375000.4355711313210954No Hit
AACCGGGCGGAAACACCA333640.3875305393439207No Hit
TGTAAACATCCCCGACTGGAAGCT324800.3772626758749114No Hit
CAACGGAATCCCAAAAGCAGCTGT310880.36109427548027234No Hit
CATTGCACTTGTCTCGGTCTGA308930.3588293055974026No Hit
CTACGCCTGTCTGAGCGTCGCT294960.3426028290519208No Hit
TTCTCACTACTGCACTTGACTAG284080.3299654586285247No Hit
TGTCAGTTTGTCAAATACCCCAA284010.3298841520173448No Hit
TACGCCTGTCTGAGCGTCGCT283460.3292453143580738No Hit
TGAGGTAGTAGTTTGTACAGTT270580.3142848979009653No Hit
GACCCGCGGGCGCTCTCCAGTCCT269530.3130652987332662No Hit
ACCGGGTGCTGTAGGCTT245650.2853281290907389No Hit
TGTAAACATCCTACACTCTCAGCT237420.27576879466201193No Hit
ACGCCTGTCTGAGCGTCGCT233470.27118077874542973No Hit
CAACACCAGTCGATGGGCTGT227790.26458332800968615No Hit
AACATTCAACGCTGTCGGTGAGTT195900.22754235900214023No Hit
ACCGGGTGCTGTAGGCTTT190610.22139790224297065No Hit
CTTCTCACTACTGCACTTGACTAG189690.2203293010674629No Hit
CCCTGTCTGAGCGTCGCT176320.20479973833209478No Hit
CCCCCACTGCTAAATTTGACTGGCTT165970.19277797510763253No Hit
TTATAAAGCAATGAGACTGATT165810.19259213142493553No Hit
TGAGGTAGTAGTTTGTGCTGTT164530.19110538196335952No Hit
TGTCAGTTTGTCAAATACCCCA163410.18980447618448051No Hit
AGAATTACTCTGAGACCT158540.18414785909239056No Hit
AACGGAATCCCAAAAGCAGCTG152620.17727164283260155No Hit
ACCGGGTGCTGTAGGCTTA149780.17397291746472976No Hit
TTCACAGTGGCTAAGTTCCG146930.17066257686668945No Hit
TAGCTTATCAGACTGATGTTGACA142050.16499434454443093No Hit
CAACGGAATCCCAAAAGCAGCT135920.1578742084511021No Hit
TTCAAGTAATCCAGGATAGGCTT134920.15671268543424585No Hit
TGTAAACATCCTACACTCAGCT129090.14994100624597387No Hit
TTCAAGTAATCCAGGATAGGC127980.14865171569726343No Hit
CCCCCCACTGCTAAATTTGACTGG123230.14313448136719623No Hit
TAGCAGCACGTAAATATTGGC120180.13959183616578463No Hit
ACTGGACTTGGAGTCAGAAGG119290.13855808068078257No Hit
TGAGGTAGTAGGTTGTGTGGTT118360.13747786427510625No Hit
GCGTTCGCGCTTTCCCCTG116990.13588657774201318No Hit
CTCCCACTGCTTCACTTGACTAG115790.1344927501217857No Hit
CCCCCCACTGCTAAATTTGACTGGCT115030.13360999262897494No Hit
CAAAGAATTCTCCTTTTGGGCT111980.13006734742756335No Hit
TGTAAACATCCCCGACTGGAAGC110310.12812760398941342No Hit
ACCACAGGGTAGAACCACGGAC107310.12464303493884465No Hit
TATTGCACTTGTCCCGGCCTGTT106370.12355120330299978No Hit
TGAGGTAGTAGGTTGTATAGT103760.12051962822900496No Hit
AGCAGCATTGTACAGGGCTATG103360.12005501902226244No Hit
CACCCGGCTGTGTGCACATGTGC103090.11974140780771127No Hit
TAGTGGGTTATCAGAACT100750.11702344394826762No Hit
AGAGGTAGTAGGTTGCATAGTT100140.11631491490798532No Hit
CTCGACACAAGGGTTTGT99650.11574576862972574No Hit
CACTAGATTGTGAGCTCCTGGA98330.1142125582474755No Hit
AGCAGCATTGTACAGGGCTATGT97830.11363179673904737No Hit
ACTGGACTTGGAGTCAGAAGGCT94580.10985684693426453No Hit
TTCACAGTGGCTAAGTTCTGC94370.10961292710072472No Hit
TAGCAGCACGTAAATATTGGCGT94200.10941546818785915No Hit
TTCACAGTGGCTAAGTTCTG94010.10919477881465647No Hit
TCAGTGCACTACAGAACTTTGT93360.1084397888536999No Hit
ACTGGACTTGGAGTCAGAAGGCA92770.10775449027375472No Hit
CAAAGAATTCTCCTTTTGGGCTT92240.1071388830748209No Hit
AGCTCGGTCTGAGGCCCCTCAGT91700.10651166064571853No Hit
CATAAAGTAGAAAGCACTAC87560.10170295535593361No Hit
TACCCGCGGGCGCTCTCCAGTCCT87360.10147065075256237No Hit
AATGACACGATCACTCCCGTTG87300.10140095937155101No Hit
TTCACAGTGGCTAAGTTCCGC87240.10133126799053963No Hit
CCCATAAAGTAGAAAGCACTA87240.10133126799053963No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)