FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10499656
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG107111510.201429456355521No Hit
CAACGGAATCCCAAAAGCAGCTG10184029.69938443697584No Hit
TTCAAGTAATCCAGGATAGGCT3924843.738065323282972No Hit
AACATTCAACGCTGTCGGTGAGT3774333.5947177698012203No Hit
TAGCAGCACGTAAATATTGGCG3646353.472828062176513No Hit
AAGCTGCCAGTTGAAGAACTGT3526003.358205259296114No Hit
CCCCCACTGCTAAATTTGACTG2872842.7361277359943985No Hit
TAGCTTATCAGACTGATGTTGAC2085511.9862650738271808No Hit
AACATTCAACGCTGTCGGTGAG2072831.9741884876990257No Hit
TAGCTTATCAGACTGATGTTGACT2068991.9705312250229912No Hit
TATTGCACTTGTCCCGGCCTGTAA1734361.6518255455226343No Hit
TGTAAACATCCTTGACTGGAAGCT1513341.4413234109765118No Hit
TATTGCACTTGTCCCGGCCTGTAT1482891.412322460850146No Hit
TGAGGTAGTAGGTTGTATAGTT1231941.1733146304983706No Hit
AGCAGCATTGTACAGGGCTATGA1007390.9594504810443314No Hit
TAGCTTATCAGACTGATGTTGA959150.91350611867665No Hit
TGTAAACATCCCCGACTGGAAGCT908570.8653331118657601No Hit
CAACGGAATCCCAAAAGCAGCTGT783360.746081585911005No Hit
ACTGGACTTGGAGTCAGAAGGC769140.7325382850638155No Hit
TGAGGTAGTAGATTGTATAGTT665140.6334874209212188No Hit
CCCATAAAGTAGAAAGCACTAC646150.6154011140936426No Hit
TATTGCACTTGTCCCGGCCTGT644170.6135153380263125No Hit
CATAAAGTAGAAAGCACTACT590540.56243747414201No Hit
CCCCCACTGCTAAATTTGACTGGCTT568170.5411320142297995No Hit
TATTGCACTTGTCCCGGCCTGTA547100.5210646901193715No Hit
TGTAAACATCCTACACTCTCAGCT540340.5146263839501027No Hit
TTCAAGTAATTCAGGATAGGTT507920.4837491818779587No Hit
CCCCCCACTGCTAAATTTGACTGGCT434720.4140326121160541No Hit
CAAAGAATTCTCCTTTTGGGCT410370.39084137613651343No Hit
TAGCTTATCAGACTGATGTTGACA350000.33334425432604653No Hit
CTTCTCACTACTGCACTTGACTAG337270.32122004759013056No Hit
AACATTCAACGCTGTCGGTGAGTT320680.305419529935076No Hit
TTCTCACTACTGCACTTGACTAG316000.30096224104865915No Hit
TGAGGTAGTAGTTTGTACAGTT312040.2971906889139987No Hit
CACCCGGCTGTGTGCACATGTGC290090.2762852421069795No Hit
CAAAGAATTCTCCTTTTGGGCTT287400.27372325340944503No Hit
CATTGCACTTGTCTCGGTCTGA286190.2725708347016321No Hit
CCCATAAAGTAGAAAGCACT282760.2693040610092369No Hit
AACCGGGCGGAAACACCA281540.26814211817987177No Hit
CCCCCCACTGCTAAATTTGACTGG274030.26098950289419004No Hit
CAACGGAATCCCAAAAGCAGCT257590.24533184706241806No Hit
CGCCTGTCTGAGCGTCGCT245310.23363622579634988No Hit
ACCCCCCACTGCTAAATTTGACTG243540.2319504562816153No Hit
TTCAAGTAATCCAGGATAGGCTT239310.22792175286504623No Hit
ACTGGACTTGGAGTCAGAAGGCA235340.22414067660883363No Hit
AACGGAATCCCAAAAGCAGCTG235300.22410258012262496No Hit
CACTAGATTGTGAGCTCCTGGAA228630.21774999104732573No Hit
AGCTCGGTCTGAGGCCCCTCAGT221720.21116882305477436No Hit
TGTAAACATCCCCGACTGGAAGC219710.20925447462278762No Hit
TAGCAGCACGTAAATATTGGC219550.20910208867795285No Hit
AGCAGCATTGTACAGGGCTATGT199930.19041576219258993No Hit
CTCCCACTGCTTCACTTGACTAG198420.18897761983821185No Hit
CACTAGATTGTGAGCTCCTGGA197260.1878728217381598No Hit
GCCTGTCTGAGCGTCGCT196980.18760614633469896No Hit
ACTGGACTTGGAGTCAGAAGGCT195290.18599656979238177No Hit
CTCCCACTGCTTCACTTGACTAGCCT190990.1819011975249475No Hit
TATTGCACTTGTCCCGGCCTGTAG188550.17957731186621734No Hit
TATTGCACTTGTCCCGGCCTGTAAT179280.17074845118735318No Hit
ATCACATTGCCAGGGATTTCCA176920.1685007585010404No Hit
TGTCAGTTTGTCAAATACCCCA173190.16494826116208No Hit
TGAGGTAGTAGTTTGTGCTGTT170760.162633899624902No Hit
TGTCAGTTTGTCAAATACCCCAA168370.1603576345739327No Hit
TGTAAACATCCTACACTCAGCT155060.14768102878799078No Hit
ATCACATTGCCAGGGATTTCC152960.1456809632620345No Hit
TGAGGTAGTAGGTTGTGTGGTT152870.14559524616806493No Hit
CTACGCCTGTCTGAGCGTCGCT150360.14320469165846958No Hit
TATTGCACTTGTCCCGGCCTGTT147720.14069032356869596No Hit
CCCCCACTGCTAAATTTGACTGGCTA142880.13608064873744435No Hit
ATGCACCTGGGCAAGGATTCTGA141010.13429963800718803No Hit
TTCAAGTAATCCAGGATAGGC140790.13409010733304025No Hit
TACGCCTGTCTGAGCGTCGCT140570.13388057665889244No Hit
TATTGCACTTGTCCCGGCCTGTAAA140430.13374723895716203No Hit
TAGCAGCACGTAAATATTGGCGT139480.1328424474097056No Hit
CAACACCAGTCGATGGGCTGT138800.13219480714415788No Hit
CCCCCACTGCTAAATTTGACTGGCT131250.12500409537226745No Hit
ACCACAGGGTAGAACCACGGAC130520.12430883449895881No Hit
TATTGCACTTGTCCCGGCCTGTTT129480.12331832585753286No Hit
CCCCCCACTGCTAAATTTGACTGTA125400.11943248426424637No Hit
CAAAGTGCTGTTCGTGCAGGTAG125260.11929914656251596No Hit
AACATTCAACGCTGTCGGTGAGA118800.11314656403981235No Hit
TTATAAAGCAATGAGACTGATT116820.11126078797248215No Hit
CAGTGCAATAGTATTGTCAAAGC114020.10859403393787377No Hit
ACTGGACTTGGAGTCAGAAGG112520.10716541570504785No Hit
TTCACAGTGGCTAAGTTCTGC110160.10491772301873509No Hit
TCGTACGACTCTTAGCGG110060.10482248180321338No Hit
TGTCAGTTTGTCAAATACCCC108630.10346053242125265No Hit
AGCAGCATTGTACAGGGCTATG105170.1001651863642009No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)